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Inhibitory Effect of Methanol Extracts and Solvent Fractions from Doenjang on Mutagenicity Using in vitro SOS Chromotest and in vivo Drosophila Mutagenic System

된장 메탄올 추출물 및 분획물에 의한 in vitro SOS Chromotest 실험계와 in vivo 초파리 돌연변이 검출계에서의 항돌연변이 효과

  • Lim, Sun-Young (Division of Ocean and Science, Korea Maritime University) ;
  • Lee, Sook-Hee (Dept. of Food Science and Nutrition, Busan National University) ;
  • Park, Keun-Young (Dept. of Food Science and Nutrition, Busan National University) ;
  • Yun, Hee-Sun (Dept. of Biology, Busan National University) ;
  • Lee, Won-Ho (Dept. of Biology, Busan National University)
  • 임선영 (한국해양대학교 해양과학부) ;
  • 이숙희 (부산대학교 식품영양학과) ;
  • 박건영 (부산대학교 식품영양학과) ;
  • 윤희선 (부산대학교 생물학과) ;
  • 이원호 (부산대학교 생물학과)
  • Published : 2004.11.01

Abstract

This study investigated the inhibitory effect of methanol extracts and several solvent fractions from doen-jang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. In order to determine an antimutagenic effect of doenjang methanol extracts, other soybean fermented foods and original materials were compared. The treatment of doenjang methanol extracts (100 ${\mu}$/assay) to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 87~97% and showed higher antimutagenic effect than other fermented foods. Among solvent fractions from doenjang methanol extracts, the ethylacetate and dichloromethane fractions showed the stronger antimutagenic effect (91% and 95%, respectively) in SOS chromotest. In Drosophila mutagenic system, the treatment of ethylacetate fraction (5%/bottle) significantly inhibited aflatoxin $B_1$ induced mutagenicity by 97%. These results demonstrated that doenjang had an inhibitory effect to mutagenic agents in both in vitro and in vivo mutagenic systems, suggesting that its antimutagenic effect may be due to active compounds in the ethylacetate fraction from doenjang methanol extracts.

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