DOI QR코드

DOI QR Code

Immunopotentiating Activities of Cellular Components of Lactobacillus brevis FSB - 1

Lactobacillus brevis FSB - 1의 균체성분에 의한 면역증진 활성

  • Kim, Seong-Yeong (Dept. of Fodd Science and Biotechnology, Kyonggi University) ;
  • Shin, Kwang-Soon (Dept. of Fodd Science and Biotechnology, Kyonggi University) ;
  • Lee, Ho (Dept. of Fodd Science and Biotechnology, Kyonggi University)
  • 김성영 (경기대학교 식품생물공학과) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 이호 (경기대학교 식품생물공학과)
  • Published : 2004.11.01

Abstract

In order to evaluate the potential utilization value as a novel probiotic strain, the immunopotentiating activities of the cellular components from Lactobacillus brevis FSB-1 were examined. L. brevis FSB-1 isolated from kimchi were fractionated into the whole cell, cell wall, cytosol and extracellular preparation, and each fraction was examined on intestinal immune system modulating activity in vitro. The cell wall and cytosol preparation showed the relatively high bone marrow cell proliferating activity through Peyer's patch cell in a dose-dependent manner. But these preparations did not directly stimulate the bone marrow cell proliferation. The whole cell, cell wall and cytosol preparation also induced considerable levels of macrophage activation and mitogenicity of murine splenocytes in vitro. The anti-complementary activity (ITCH_(50)) of the cytosol fraction of L. brevis FSB-1 was the most potent in the cellular components, and the activity showed dose dependency. The complement activation by the cytosol fraction of L. brevis FSB-1 occurs via both alternative and classical pathways, which confirmed by the crossed immunoelectrophoresis using anti-human C3.

References

  1. Metchnikoff E. 1907. The prolongation of life. Optimistic studies. Wiliam Heinemann, Ltd., London
  2. Fang H, Elina T, Heikki A, Salminen S. 2000. Modulation of humoral immune response through probiotic intake. FEMS Immunol Med Microbiol 29: 47-52 https://doi.org/10.1111/j.1574-695X.2000.tb01504.x
  3. Salminen S, Ouwehand A, Benno Y, Lee YK. 1999. Probiotics: How should they be defined? Trends Food Sci Technol 10: 107-110 https://doi.org/10.1016/S0924-2244(99)00027-8
  4. Arthur C, Ouwehand PV, Kirjavainen CS, Salminen S. 1999. Probiotics: mechanisms and established effects. Int Dairy J 9: 43-52 https://doi.org/10.1016/S0958-6946(99)00043-6
  5. Sherwood L, Gorbach MD. 2000. Probiotics and gastrointestinal health. Am J Gastroenterol 95: 2-4 https://doi.org/10.1111/j.1572-0241.2000.01695.x
  6. Jung CM, Kang KH. 1999. Industrial utilization and future prospect of lactic acid bacteria. Bioindustry News (Korea) 12: 16-22
  7. Fernandes CF, Shahani KM. 1990. Anticarcinogenic and immunological properties of dietary lactobacilli. J Food Prot 53: 704-710 https://doi.org/10.4315/0362-028X-53.8.704
  8. Meydani SN, Ha WK. 2000. Immunologic effects of yogurt. Am Soc Clin Nutr 71: 861-872
  9. Bhakdi S, Klonisch T, Nuber P, Riva l, Di Fronzo G. 1997. Antiproliferative effect of fermented milk on the growth of a human breast cancer cell line. Nutr Cancer 28: 93-99 https://doi.org/10.1080/01635589709514558
  10. Kitazawa H, Harata T, Uemura J, Saito T, Kaneko T, Itoh T. 1998. Phosphate group requirement for mitogenic activation of lymphocytes by an extracellular phosphopolysaccharide from Lactobacillus delbrueckii ssp. bulgaricus. Int J Food Microbiol 40: 169-175 https://doi.org/10.1016/S0168-1605(98)00030-0
  11. Keller R, Fischer W, Keist R, Basseti S. 1992. Macrophage response to bacteria. Induction of marked secretory and cellular activities by lipoteichoic acids. Infect Immunol 60: 3664-3672
  12. Kitazawa H, Ishii Y, Uemura J, Kawai Y, Saito T, Kaneko T, Noda K, Itoh T. 2000. Augmentation of macrophage function by an extracellular phosphopolysaccharide from Lactobacillus delbrueckii ssp. bulgaricus. Food Microbiol 17: 109-118 https://doi.org/10.1006/fmic.1999.0294
  13. Uemura JN, Kitazawa H, Kawai Y, Itoh T, Oda M, Saito T. 2003. Functional alteration of murine macrophages stimulated with extracellular polysaccharides from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1. Food Microbiol 20: 267-273 https://doi.org/10.1016/S0740-0020(02)00177-6
  14. Haza AI, Zabala A, Morales P. 2003. Protective effect and cytokine production of a Lactobacillus plantarum strain isolated from ewes' milk cheese. Int Dairy J 29: 1-10
  15. Kim JH, Shin KS, Lee H. 2002. Chracterization and action mode of anti-complementary substance prepared from Lactobacillus plantarum. Kor J Food Sci Technol 34: 290- 295
  16. Elina R, Erja M, Maria S, Merja RK, Johannes A, Airi P. 2003. Probiotic and milk technological properties of Lactobacillus brevis. Int J Food Microbiol 83: 63-74 https://doi.org/10.1016/S0168-1605(02)00315-X
  17. Ouwehand AC, Tuomola EM, Tolkko S, Salminen S. 2001. Assessment of adhesion properties of novel probiotic strains to human intestinal mucus. Int J Food Microbiol 64: 119- 126 https://doi.org/10.1016/S0168-1605(00)00440-2
  18. Kandler O, Weiss N. 1986. Regular, nonsporing gram-positive rods. In Bergey's Manual of Systematic Bacteriology. Wiliams & Wilkins, Baltimore. Vol 2, p 1208-1260
  19. Kishi A, Kazuko U, Matsubara Y, Okuda C, Kishida T. 1996. Effect of oral administration of Lactobacillus brevis subsp. Coagulans on interferon-$\alpha$ producing capacity of humans. J Am Coll Nutr 15: 408-412 https://doi.org/10.1080/07315724.1996.10718617
  20. Playfair JHL. 1987. Immunology at a glance. 4th ed. Blackwell scientific publications, London
  21. Hong T, Matsumoto T, Kiyohara H, Yamada H. 1998. Enhanced production of hematopoetic growth factors through T cell activation in Peyer's patches by oral administration of Kampo (Japanese herbal) medicine, 'Juzen-Taiho-To'. Phytomed 5: 353-360 https://doi.org/10.1016/S0944-7113(98)80017-2
  22. Page B, Page M, Noel C. 1993. A new fluorometric assay for cytotoxicity measurements in vitro. Int J Oncol 3: 473- 476
  23. Suzuki I, Tanaka H, Kinoshita A, Oikawa S, Osawa M, Yadomae T. 1990. Effects of orally administered $\beta$-glucan on macrophage function in mice. Int J Immunopharmacol 12: 675-684 https://doi.org/10.1016/0192-0561(90)90105-V
  24. Zheng WH, Bastianetto S, Mennicken F, Ma W, Kar S. 2002. Amyloed $\beta$-peptide induces tau phosphorylation and loss of cholinergic neurons in rat primary septal cultures. Neurosci 115: 201-211 https://doi.org/10.1016/S0306-4522(02)00404-9
  25. Kabat EA, Mayer MM. 1971. Complement and complement fixation. In Experimental Immunochemistry. 2nd ed. Charles, C, ed. Thormas Publisher, Illinois, USA. p 133-240
  26. Shimura K, Ito H, Hibasami H. 1983. Screening of host- mediated antitumor polysaccharides by crossed immunoelectrophoresis using fresh human serum. Jpn J Pharmacol 33: 403-408 https://doi.org/10.1254/jjp.33.403
  27. Benedetti R, Massouh E, Flo J. 1995. The bone marrow as a site of antibody production after a mucosal immunization. Immunol Lett 48: 109-115 https://doi.org/10.1016/0165-2478(95)02453-0
  28. Kim DS, Kim SY, Kim CC, Min CG, Park SJ, Seo YH, Yeum HS, Jung NG, Jung DC, Jung TJ, Jin JY, Han CH. 2000. Immunological biotechnology. 4th ed. Lifescience, Oxford
  29. Janeway CA, Travers P, Walport M. 1994. The immune system in health and disease. Garland Publisher, New York
  30. Yasui H, Ohwaki M. 1991. Enhancement of immune response in Peyer's patch cells cultured with Bifidobacterium breve. J Dairy Sci 74: 1187-1195 https://doi.org/10.3168/jds.S0022-0302(91)78272-6
  31. Shin MS, Yu KW, Shin KS, Lee H. 2004. In vitro bone marrow cell proliferation of cell wall preparation from Bifidobacterium bifidum SL 21. Kor J Food Sci Technol 36: 484- 489
  32. Benjamini EG, Lesrowitz S. 1996. Immunology a short course. 3th ed. Wileyliss, New York
  33. Abbas AK, Lichtman AH, Pober JS. 2000. Cellular and Molecular Immunology. 4th ed. WB Sounders, London
  34. Wright PFA, Kirjavainen PV, Ahokas JT, Salminen SJ. 1998. Effect of orally-dosed viable probiotics on mouse lymphocyte proliferation. Toxicol Lett 95: 157
  35. Kitazawa H, Watanabe H, Shimosato T, Kawai Y, Itoh T, Saito T. 2003. Immunostimulatory oligonucleotide, CpG- like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAI B6. Int J Food Microbiol 85: 11-21 https://doi.org/10.1016/S0168-1605(02)00477-4
  36. Kwon MH, Sung HJ. 1997. Characteristics of immune response by polysaccharides with complement system activity. Food Sci Indus 30: 30-43
  37. Jung YJ, Chun H, Kim KI, An JH, Shin DH, Hong BS, Cho HY, Yang HC. 2002. Purified polysaccharide activating the complement system from leaves of Diospyos kaki L. Kor J Food Sci Technol 34: 879-884
  38. Whaley K. 1986. The complement system. In Complement in health and disease. Whaley K, ed. MTP Press, Lancaster, USA. p 1-35
  39. Shin KS. 1999. Potential utilization and medical activity of pectic substance. Food Sci Indus 32: 91-101

Cited by

  1. Physicochemical components and antioxidant activity of Sparassis crispa mixture fermented by lactic acid bacteria vol.23, pp.3, 2016, https://doi.org/10.11002/kjfp.2016.23.3.361
  2. Adhesive Properties of Lactobacillus brevis FSB-1 In Vivo vol.30, pp.4, 2010, https://doi.org/10.5851/kosfa.2010.30.4.560
  3. Effects of Dietary Probiotics as an Alternative to Antibiotics on Growth Performance, Biochemical Characteristics and Immune Response in Weaning Pigs vol.24, pp.4, 2014, https://doi.org/10.5352/JLS.2014.24.4.352
  4. Antioxidative Activity of Mushroom Water Extracts Fermented by Lactic Acid Bacteria vol.43, pp.1, 2014, https://doi.org/10.3746/jkfn.2014.43.1.080
  5. Health benefits of lactic acid bacteria isolated from kimchi, with respect to immunomodulatory effects vol.24, pp.3, 2015, https://doi.org/10.1007/s10068-015-0102-3