DOI QR코드

DOI QR Code

Resazurin Reduction Time Test to Determine Post-pasteurization Contamination and Shelf Life of Market Milk

시유의 2차오염과 저장가능기간을 결정하기 위한 Resazurin 환원시간검사

  • Choi, S,H. (Dept of Biotechnology Sangji University) ;
  • Choi, J.J. (Dept of Biotechnology Sangji University) ;
  • Lee, S.B. (Dept of Biotechnology Sangji University) ;
  • Yoon, Y.H. (Dept. of Animal Science and Technology Chung-Ang University)
  • 최석호 (상지대학교 생명공학과) ;
  • 최정준 (상지대학교 생명공학과) ;
  • 이승배 (상지대학교 생명공학과) ;
  • 윤영호 (중앙대학교 동물자원과학과)
  • Published : 2004.12.31

Abstract

The selective media including NPC agar, DHL agar, MacConkey agar, and Cetrimide desoxycholate agar were compared to determine selectivity for the growth of bacteria Cetrimide desoxycholate agar was better than NPC agar, DHL agar, and MacConkey agar for the growth of psychrotrophic grarn-negatve bacteria including Pseudomonas. and for the inhibition of gram positive bacteria The specificity of resazurin reduction time test was investigated to determine post-pasteurization contamination of market milk. Equal volume of Cetrimide desoxycholate broth was added to market milk, which was then incubated at $21^{\circ}C$ for 18 hours. The growth of bacteria in the incubated milk was detected in resazurin reduction time test. The results in resazurin reduction time test and total bacteria number count of market milk after storage at $7^{\circ}C$ were relatively correlated each other. Pseudomonas was isolated most frequently from the market milk stored at $7^{\circ}C$ for 10 days, and Acinetobacter and Aeromonas followed. Acinetobacter, Pseudomonas and Enterobacter were frequently isolated from the mixture of market milk and Cetrimide desoxycholate broth incubated at $21^{\circ}C$ for 18hours in resazurin reduction time test.

NPC agar, DHL agar, MacConkey agar와 Cetrimide desoxycholate agar의 선택성을 조사 하였다". Cetrimide desoxycholate agarz 가 다른 선택성 배지에 비해 그람양성 세균을 억제하고 Pseudomonas를 비롯한 내냉성 그람음성 세균의 선택적 배양에 더 좋은 한천배지이었다. Resazurin 환원시간 검시에서 Cetrimide desoxycholate broth를 시유와 혼합하여 $21^{\circ}C$ 에 서 18 시간 배양한 후 resazurin을 첨가하여 변색되는 시간으로 2차오염 을 결정하는 방법으로서 조사하였다. Resazurin 환원시간 검사와 저장성 검사간에 81%의 관련성이 있었다. 저장성 검사의 우유에서 Pseudomonas가 가장 먼도 높게 분리되었으며 다음으로 Acinetobacter와 Aeromonas가 검출되었다 Resazurin 환원시간 검사에는 Acinetobacter, Pseudomonas, Enterobacter의 순서의 빈도로 검출되었다.

References

  1. Anonymity. 1998. API 20E - Identification system for Enterobacteriaceae and other gram-negative rods. bioMerieux sa.
  2. Atlas, R. M. and Parks, L. C. 1993. Handbook of microbiological media. CRC Press. Boca Raton
  3. Bishop, J. R. and White, C. H. 1986. Assessment of dairy product quality and potential shelf life - a review. J. Food Prot. 49:739-753. https://doi.org/10.4315/0362-028X-49.9.739
  4. Bishop, J. R., White, C. H. and Firstenberg-Eden, R. 1984. Rapid impedimetric method for determining the potential shelf-life of pasteuried whole milk. J. Food Prot. 47:471-475.
  5. Bossuyt, R. G. and Waes, G. M. 1983. Impedance measurements to detect post-pasteurization contamination of pasteurized milk. J. Food Prot. 46:622-624. https://doi.org/10.4315/0362-028X-46.7.622
  6. Byrne, Jr, R. D., Bishop, J. R. and Boling, J. W. 1989a. Estimation of potential shelf-life of pasteurized fluid milk utilizing a selective preliminary incubation. J. Food Prot. 52:805-807.
  7. Byrne, R. D., Bishop, J. R. and McGilliard, M. L. 1989b. Selective preliminary incubation for gram-negative psychrotrophic bacteria in milk. J. Food Prot. 52:805-807.
  8. Gruetmacher, T. J. and Bradley, R. L. Jr. 1999. Identification and control of precessing variables that affect the quality and safety of fluid milk. J. Food Prot. 62:625-31. https://doi.org/10.4315/0362-028X-62.6.625
  9. IDF. 1993. Catalogue of tests for the detection of post-pasteurization contamination of milk. Bulletin of the IDF 281:13-34.
  10. Langeveld, L. P. M., Cuperus, F., van Breeman, P. and Dijkers, J. 1976. A rapid method for the detection of post-pasteurization contamination in HTST-pasteurized milk. Neth. Milk Dairy J. 30:157-173.
  11. MacFaddin, J. F. 1980. Biochemical tests for identification of medical bacteria, 2nd. ed. Williams and Wilkins, Baltimore.
  12. Phillips, J. D., Griffiths, M. W. and Muir, D. O. 1984, Preincubation test to rapidly identify post-pasteurization contamination in milk and single cream. J. Food Prot. 5:391-393.
  13. Visser, I. J. R. and de Groote, J. M. F. H. 1984. The Malthus microbiological growth analyzer as an aid in the detection of post-pasteurization contamination of pasteurized milk. Neth. Milk Dairy J., 38:151-156.
  14. Waes, G. M. and Bossuyt, R. G. 1982. Usefulness of the benzalkon-crystal violet-ATP method for predicting the keeping quality of pasteurized milk. J. Food Prot., 45:928-931. https://doi.org/10.4315/0362-028X-45.10.928
  15. White, C. H., Bishop, J. R. and Morgan, D. M. 1993. Microbiological methods for dairy products. In Marshall, R. T. ed. Standard methods for the examination of dairy products. pp. 287-308. American Public Health Association.
  16. Zhiyong, L., Minying, Y. and Jianting, G. 2003. Rapid impediance method for predicting the po-tential shelf life of packaged pasteurized milk. J. AOAC Int. 86:998-1002.
  17. 최석호, 권우혁, 최정준, 이승배. 1999a. ATP bioluminescence와 전기저항법을 이용한 시유의 2차오염 검출법에 관한 연구. Korean J. Dairy Sci. 21:221-230.
  18. 최석호, 최정준, 이승배. 1999b. 색소환원법에 의한 시유의 2차오염 검출을 위한 그람음성 세균 선택성 배지. Korean J. Korean Sci. 21:231-240.