Expression on Echinostoma hortense Infection Manifestation on the Cytokine of the Splenocytes of BALB/c and C3H/HeN Mice

  • Ryang Yong-Suk (Department of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei University) ;
  • Cho Yoon-Kyung (Department of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei University) ;
  • IM Jee-Aee (Department of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei University) ;
  • Lee Kyu-Je (Department of Parasitology, Wonju College of Medicine, Yonsei University) ;
  • Kim Insik (Department of Clinical Laboratory Science, School of Medicine, Eulji University) ;
  • Sung Ho-Joong (Department of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei University)
  • Published : 2004.09.01

Abstract

This experiment was performed to examine the in vitro and in vivo affects of the two different haplotype strains of mice, BALB/c and C3H/HeN infected with Echinostoma hortense, and the manifestation of the profiles of cytokine in the splenocytes. In the in vitro experiment, the two mice's splenocytes were divided and stimulated with antigen of crude extracts and the antigen of excretory and secretory products of an adult warm and the manifestation of cytokine mRNA was verified with RT-PCR. As a result, the two different strains of mice both strongly manifested the Th2 cytokine rather than the Thl cytokine and in the case of the Th2 cytokine, the BALB/c mice manifested more strongly than the C3H/HeN mice. In the experiment using the ELISA method, the protem cytokine manifestation had the same result as the mRNA experiment. In the in vivo experiment, the mice was infected via oral route with the metacercaria of the Echinostoma hortense and the manifestation of cytokine was verified by RT-PCR and ELISA and the results were the same as the in vitro experiment. Therefore, in the two strains of BALB/c and C3H/HeN, the C3H/HeN showed a higher susceptivity to the Echinostoma hortense.