Inorganic Phosphate Solubilization by Immobilized Pantoea agglomerans under in vitro Conditions

고정화된 Pantoea agglomerans에 의한 난용성 인산의 가용화

  • Kim, Eun-Hee (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Park, Sung-Ae (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Park, Myoung-Su (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Yang, Jin-chul (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Seshadri, Sundaram (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2003.12.06
  • Accepted : 2004.02.05
  • Published : 2004.02.29

Abstract

It is now widely accepted that immobilized microbial cells can overcome some of the problems associated with microbial survival stability, efficacy, storage, transportation and ease of application in agricultural environments. Pantoea agglomerans, a phosphate solubilizing bacterium, was immobilized in alginate, agar and gelatin carriers. All the three immobilfized carriers with bacterial cells of P. agglomerans were compared for solubilization of tricalcium phosphate in pure liquid cultures. While alginate beads were tested for phosphate solubilization on alternate days up to five days, agar beads and gelatin cubes were subjected for one time phosphate solubilization analysis after seven days. Both alginate and agar immobilized cells of P. agglomerans exhibited higher efficiency in increasing the solubilizaliun of tricalcium phosphate than gelatin immobilized cells. The culture filtrate of alginate bead inoculation treatment registered a rapid increase in soluble phosphate concentration upon incubation. A corresponding decrease in the pH of the medium was also observed in all the treatments.

Acknowledgement

Supported by : Ministry of Agriculture and Forestry

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