Inorganic Phosphate Solubilization by Immobilized Pantoea agglomerans under in vitro Conditions

고정화된 Pantoea agglomerans에 의한 난용성 인산의 가용화

  • Kim, Eun-Hee (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Park, Sung-Ae (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Park, Myoung-Su (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Yang, Jin-chul (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Seshadri, Sundaram (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2003.12.06
  • Accepted : 2004.02.05
  • Published : 2004.02.29


It is now widely accepted that immobilized microbial cells can overcome some of the problems associated with microbial survival stability, efficacy, storage, transportation and ease of application in agricultural environments. Pantoea agglomerans, a phosphate solubilizing bacterium, was immobilized in alginate, agar and gelatin carriers. All the three immobilfized carriers with bacterial cells of P. agglomerans were compared for solubilization of tricalcium phosphate in pure liquid cultures. While alginate beads were tested for phosphate solubilization on alternate days up to five days, agar beads and gelatin cubes were subjected for one time phosphate solubilization analysis after seven days. Both alginate and agar immobilized cells of P. agglomerans exhibited higher efficiency in increasing the solubilizaliun of tricalcium phosphate than gelatin immobilized cells. The culture filtrate of alginate bead inoculation treatment registered a rapid increase in soluble phosphate concentration upon incubation. A corresponding decrease in the pH of the medium was also observed in all the treatments.


Supported by : Ministry of Agriculture and Forestry


  1. de Alteriis, E,. P. Parascandola, S. Salvadore, and V. Sardi. 1985. Enzymc immobilization within insolubilized gelatin. J. Chem. Technol. Biot. 35(B):60
  2. Ehrlich, H. L. 1990. Mikrobiologische und biochemische Verfahren stechnik, In A. Einsele et al. (ed.) Geomicrobiology, 2nd ed. VCH Verlagsgesellschafat, Weinheim, Germany
  3. Hinsinger, P. 2001. Bioavailability of soil inorganic P in the rhizosphere as affected by root induced chemical changes: a review. Plant Soil 237:173-195
  4. Lopez, A., N. Lazaro, and A. M. Marques. 1997. The interphase technique: a simple method of cell immobilization in gel beads. J. Microbiol. Meth. 30:231-234
  5. Pikovskaya, R. I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya 17:362-370
  6. Vassileva, M., R. Azcon, J. M. Barea, and N. Vassilev. 1998. Application of an encapsulated filamentous fungus in solubilization of inorganic phosphate. J. Biotechnol. 63:67-72
  7. Vassilev, N., M. Vassileva, R. Azcon, and J. M. Barea. 2001. Interactions of an arbuscular mycorrhizal fungus with free or coencapsulated cells of Rhizobium trifoli and Yarowia lipotytica inoculated into a soil plant system. Biotechnol. Lett. 23:149-151
  8. Woodward, J. 1988. Methods of immobilization of microbial cells. J. Miciobiol. Meth. 8:91-102
  9. Vassilev, N., M. Toro, M. Vassileva, R. Azcon, and J. M. Barea. 1997b. Rock phosphate solubilization by immobilized cells of Enterobacter sp. in fermentation and soil conditions. Bioresource Technol. 61:29-32
  10. Vassileva, M., R. Azcon, J. M. Barea, and N. Vassilev. 2000. Rock phosphate solubilization by free and encapsulated cells of Yarowia lipolytica. Process Biochem. 35:693-697
  11. Fedirici, F. 1993. Potential application of viable, immobilized fungal cell systems. World J. Microb. Biot. 9:495-502
  12. Goldstein, A. H., R. D. Rogers, and G. Mead. 1993. Separating phosphate from ores via bioprocessing. Nat. Biotechnol. 11:250-254
  13. Van Elsas, J. D., and C. E. Heijnen. 1990. Methods for the introduction of bacteria into soil: a review. Biol. Fert. Soils 10:127-133
  14. Zayed, G. 1997. Can immobilization of Bacillus megaterium cells in alginate beads protect them against bacteriophages. Plant Soil 197:1-7
  15. Rodrigues, H., and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-339
  16. Bashan, Y., and L. E. Gonzalez. 1999. Long tern survival of the plant growth promoting bacteria Azospirillum brasilense and Pseudomonas fluorescence in dry alginate inoculant. Appl. Microbiol. Biot. 51:262-266
  17. Deelereck, S., D. G. Strullu, C. Plenchette, and T. Guillemette. 1996. Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials. J. Biotechnol. 48:51-57
  18. Fenice, M., L. Selbman, F. Federici, and N. Vassilev. 2000. Application of encapsulated Penicillium variabile P16 in solubilization of rock phosphate. Bioresource Technol. 73:57-162
  19. Murphy, J., and J. P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta. 27:31-36
  20. Chung, H. K. 2003. Identification of phosphate solubilizing bacteria isolated from rhizosphere. M.S. Thesis, Chungbuk National University, Cheongju, Korea
  21. Bashan, Y. 1998. Inoculants of plant growth promoting bacteria for use in adriculture. Biotechnol. Adv. 16:729-770
  22. Vassilev, N., M. Vassileva, and R. Azcon. 1997a. Solubilization of rock phosphate by immobilized Aspergillus niger. Bioresource Technol. 59:1-4