Development of PCR-dot blot hybridization for the diagnosis of alcelaphine herpesvirus 1

Alcelaphine herpesvirus 1 진단을 위한 PCR-dot blot hybridization의 개발

  • Kim, Okjin (Department of Laboratory Animal Sciences and Center for Animal Resource Development,College of Medicine, Seoul National University) ;
  • Li, Hong (USDA - ARS, Animal Disease Research Unit)
  • 김옥진 (서울대학교 의과대학 실험동물학교실, 서울대학교 의과대학 특수생명자원 연구센타) ;
  • Accepted : 2003.10.07
  • Published : 2004.03.31

Abstract

The aim of the present study was to develop a sensitive and specific assay for the diagnosis of alcelaphine herpesvirus 1 (AlHV-1) which is a cause agent of malignant catarrhal fever in ruminants. A1HV-1 is a gamma herpesvirus, which is frequent latent, and it is often difficult to detect its antigens or specific nucleic acids because of its low genomic copies in the infected tissues. In this study, polymerase chain reaction (PCR)-dot blot hybridization (DBH) assay for detecting AlHV-1 DNA was developed and evaluated for its sensitivity and specificity as comparison with PCR and DBH alone. The developed PCR-DBH was more sensitive than PCR or DBH alone and also very specific. The results showed that the sensitivity of PCR-DBH were higher and stronger than those of PCR and DBH alone. This PCR-DBH assay can be applied efficiently to confirm the presence of AlHV-1 virus on clinical samples and to differentiate specifically between AlHV-1 infection and other viral infections.

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