Cytochrome $C_{550}$ is Related to Initiation of Sporulation in Bacillus subtilis

  • Shin Inji (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Ryu Han-Bong (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Yim Hyung-Soon (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Kang Sa-Ouk (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University)
  • Published : 2005.06.01

Abstract

The effect of cytochrome $c_{550}$ encoded by cccA in Bacillus subtilis during the event of sporulation was investigated. The sporulation of cccA-overexpressing mutant was significantly accelerated, while disruptant strain showed delayed sporulation in spite of the same growth rate. Activity of sporulation stage-0-specific enzyme, extracellular $\alpha-amylase$ of mutant strains was similar to that of the control strain, but cccA-overexpressing mutant exhibited higher activity of stage-II-specific alkaline phosphatase and stage-III-specific glucose dehydrogenase when compared to deletion mutant and control strain. Northern blot analysis also revealed that cccA-overexpressing mutant showed high level of spo0A transcripts, while the disruptant rarely expressed spo0A. These results suggested that although cytochrome $c_{550}$ is dispensable for growth and sporulation, expression of cccA may play an important role for initiation of sporulation through regulation of spo0A expression.

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