Antioxidant and Anticancer Activities of Ginseng Treated with Traditional Rice Wine Steam Process Method

전통적 탁주증자법으로 처리한 홍삼의 일부 항산화 및 항암효과

  • Ye, Eun-Ju (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Kim, Soo-Jung (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Park, Chang-Ho (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Bae, Man-Jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University)
  • 예은주 (대구한의대학교 한방바이오 식품과학과) ;
  • 김수정 (대구한의대학교 한방바이오 식품과학과) ;
  • 박창호 (대구한의대학교 한방바이오 식품과학과) ;
  • 배만종 (대구한의대학교 한방바이오 식품과학과)
  • Published : 2005.06.01


The aim of this study was to develop the new processing method for ginseng. To investigate the efficacy of the new product (the traditional rice wine steamed-red ginseng: RWS-RGS), antioxidant and anticancer effects of RWS-RGS were examined. The DPPH radical scavenging effect of RWS-RGS extracted with ethanol was increased in dose-dependent manner Especially, A3 ($3^{rd}$ traditional rice wine steamed-red ginsengs) exhibited effective DPPH radical scavenging activity. Nitrite scavenging effect of white ginseng (W.G), red ginseng (R.G) and RWS-RGS ($A1\~A9:\;1^{st}$ traditional rice wine steamed-red $ginseng\~9^{th}$ traditional rice wine steamed-red ginseng) were $25.9{\pm}4.4\%,\;12.9{\pm}1.1\%\;and\;26.2{\pm}0.1\~56.1{\pm}0.6\%$ at pH 1.2, respectively. The antitumor effects of W.G, R.G and RWS-RGS (A9) were examined in Hep3B cancer cells. Their growth inhibition against Hep3B cancer cells showed $19.6{\pm}4.5\%,\;54.5{\pm}6.1\%,\;96.3{\pm}2.4\%$ at 5,000 ppm, respectively. These result suggest that the traditional rice wine steamed ginseng will be useful product with antioxidant and antitumor effect.


  1. Hu SY. 1976. The genus Panax (Ginseng) in Chinese medicine. Economic Botany 30: 11-28
  2. Kwak HS, Joo CN. 1980. Effect of ginseng saponin fraction on ethanol metabolism in rat liver. Korean J Ginseng Sci 12: 76-81
  3. Takagi K. 1974. Pharmacological studies in ginseng. Proc. 1st Intl. Ginseng Symp. Seoul, Korea. p 119-127
  4. Kim ND, Han BH, Lee EB, Kong JY. 1979. Studies on ginseng on antistress effects. Korean J Pharmacog 10: 61-65
  5. Kim MJ, Jung NP. 1987. The effect of ginseng saponin fractions on mouse immune system. Korean J Ginseng Sci 11: 130-135
  6. Park HW, Kim SC, Jung NP. 1988. The effect of ginseng saponin factions on humoral immunity of mouse. Korean J Ginseng Sci 12: 63-68
  7. Saito H, Lee YM. 1978. Pharmacological properties of panax ginseng root. Proc. 2nd Intl. Ginseng Symp, Seoul, Korea. p 109-114
  8. Park MG. 1996. Korean ginseng. Korea ginseng and tobacco research institute, Daejeon, Korea
  9. Han BH, Park MW, Woo LK, Woo WS, Han YN. 1978. Studies on the antioxidant components of Korean ginseng. Proc. 2nd Intl. Ginseng Symp., Seoul, Korea. p 13-15
  10. Yammamoto M, Hyashi Y, Kumagai A. 1980. Lipid metabolism and several saponin principles. Proc. 3rd Intl. Ginseng Symp., Seoul, Korea. p 115-116
  11. Shin HR. 2000. The cancer-preventive potential of Panax ginseng a review of human and experimental evidence. Cancer Causes and Control 11: 565-576
  12. Yun TK. 1996. Experimental and epidemiological evidence of the cancer-preventive effects of Panax ginseng C.A. Meyer. Nutr Rev 54: S71-81
  13. Chen X, Liu H, Lei X, Fu Z, Li Y, Tao L, Han R. 1998. Cancer chemopreventive and therapeutic activities of red ginseng. J Ethnopharmacol 60: 71-78
  14. Kim ND, Kang SY, Park JH, Schini-Kerth VB. 1999. Ginsenoside $Rg_3$ mediates endothelium-dependent relation in response to ginsenosides in rat aorta: role of $K^+$ channels. Eur J Pharmacology 367: 41-49
  15. Kim ND, Kang SY, Kim MJ, Park JH, Schini-Kerth VB. 1999. The ginsenoside $Rg_3$ evokes endothelium-independent relaxation in rat aortic rings: role of $K^-$ channels. Eur J Pharmacology 367: 51-57
  16. Lee SR, Park JH, Choi KJ, Kim ND. 1997. Inhibitory effects of ginsenoside $Rg_3$ on platelet aggregation and its mechanism of action. Korean J Ginseng Sci 21: 132-140
  17. Lee KY, Lee YH, Kim SI, Park JH, Lee SK. 1997. Ginsenoside Rg5 suppresses cyclin E-dependent protein kinase activity via up-regulating p21 cip/WAF and down-regulating cyclin E in sk-Hep-1 cells. Anticancer Research 17: 1067-1072
  18. Kim SE, Lee YH, Park JH, Lee SK. 1999. Ginsenoside Rs4, a new type of ginseng saponin concurrently induces apoptosis and selectvely elevatates protein levels of p53 and p21 WAFI in human hepatoma sk- Hep-l cells. Eur J Cancer 35: 507-511
  19. Kim SE, Lee YH, Park JH, Lee SK. 1999. Ginsenoside Rs3, a new dial-type ginseng saponin, selectvely elevatates protein levels of p53 and p21 WAFI leading to introduction of apoptosis sk-Hep-l cells. Anticancer Research 19: 487-492
  20. Kim CS, Choi KJ, Yang JW, Kim SB. 2000. Effect of preheating condition of raw ginseng on the yeild and physical property of Korean red ginseng extract. Korean J Medicinal Crop Sci 8: 146-150
  21. Park SY, Jung I, Kang TL, Park MK. 2001. Difference between steaming and decocting ginseng. J Ginseng Res 25: 37-40
  22. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH. 2000. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod 63: 1702-1704
  23. Ro JS, Hwang SY, Hwang BH. 2001. Quantitative determination of 5-hydroxymethyl-2-furaldehyde in the Rehmanniae Radix Preparata samples at various processing stages. Kor J Pharmacogn 32: 116-120
  24. Lee KS, Ze KR, Hong SY, 1990. Studies on the extraction quantity of the specific components of crude drug preparation based on prescription. National Institute of Health 27: 326-331
  25. Park NK, Kim SL, Hur HS, Park CH. 2002. Development of R. Radix preparata with new variety 'Jiwhang 1'. Kor J Intl Agri 14: 34-39
  26. Lee JH, Koh JA, Hwang EY. 2002. Quantitative determination of 5-hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to various processings. Kor J Herbology 17: 145-149
  27. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1198
  28. Choi JH, Oh SK. 1983. Studies on the anti-agiging action of Korean ginseng. Korean J Food Nutr 12: 323-335
  29. Kato H, Lee lE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formmation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338
  30. Kim DS, Ahn BW, Teum DM, Lee DB, Kim SB, Park YH. 1987. Degration of carcinogenic nitrisamine formation factor by natural food components: Nitrite-scavenging effects of vegetable extracts. Bull Korean Fish Soc 20: 463-468
  31. Kim DC, Chang SM, Choi J. 1995. Variation of effective constituents contents, physical properties and color intensities of extracts from white ginseng roots of different cultivating years. Agric Chem Biotechnol 38: 67-71
  32. 中林敏朗, 木村進, 加藤博通 1967. 食品の變色とその化學. 光琳書院, 東京. p 223-289
  33. Do JH, Kim KH, Jang JG, Yang JW, Lee KS. 1989. Changes in color intensity and components during browning reaction of white ginseng water extract. Korean J Food Sci Technol 21: 480-485
  34. Shank RC. 1975. Toxicology of N-nitro compounds. Toxicol Appl Pharmacol 31: 361-368
  35. Kim SB, Lee DH, Yeum DJ, Park JW, Park YH. 1988. Nitrite scavengin effect of mailard reaction products derived from glucose-amono acids. Korean J Food Sci Technol 20: 453-458
  36. Choi DY. 1999. Effect of growth inhibition in Hep3B cell and HeLa cell by treatment of Euonymus alatus (Thunb.) Sieb extracts. Dongguk J Institute of Oriental Medicine 7: 155-162

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