DOI QR코드

DOI QR Code

Cytotoxicity and Multidrug -Resistance Reversing Activity of Extracts from Gamma-Irradiated Coix Zachryma-jobi L. var. ma-yuen Stapf Seed

감마선 조사된 율무종자의 세포독성 및 다제내성 극복활성

  • Cha, Young-Ju (Biology Research Center of industrial Accelerators, Dongshin University) ;
  • Lee, Sook-Young (Biology Research Center of industrial Accelerators, Dongshin University)
  • 차영주 (동신대학교 산업용가속기이용생물연구센터) ;
  • 이숙영 (동신대학교 산업용가속기이용생물연구센터)
  • Published : 2005.06.01

Abstract

This study was carried out to examine the effects of gamma irradiation on the cytotoxicity and multidrug-resistance reversing activity of methanol extracts from Coix lachryma-jobi L. var. me-yuen Stapf seed. The seed was irradiated with doses of 1, 4, 8, 16, 32 and 64 Gy of the gamma radiation, and then extracted by methanol. The extracts were examined for cytotoxicity on the human cancer cell lines, MCF-7 (human breast adenocarcinoma pleural effusion), Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells, and investigated for multidrug-resistance reversing activity using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. The growth inhibitory activity of irradiated seed extracts on human cancer cell lines was higher than that of the control. In the case of Calu-6 cell line, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. $IC_{50}$ value in the MCF-7 cell line was measured in the only 8 Gy extract. And in the SNU-601 cell line as Calu-6, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. But the extracts of gamma-irradiated seed over 32 Gy showed little growth inhibitory effect against human cancer cell lines. In this result, 8 Gy extract had significant growth inhibitory in all human cancer cell lines $(Calu-6:\;633\;{\mu}g/mL,\;MCF-7:\;653\;{\mu}g/mL\;and\;SNU-601:\;683\;{\mu}g/mL)$. The extracts of 4, 8 and 16 Gy strongly potentiated vincristine cytotoxicity in AML-2/D100 cells. The reversal fold (RF) of 4, 8 and 16 Gy extracts was 1.7, 1.8 and 1.6, respectively. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. These results indicate that the above samples would contain some principles which have cytotoxicity and multidrug-resistance reversing activity. Irradiation technology can be applied to promote physiological activities of medicinal plant seeds.

References

  1. Chin KD. 1975. Special lecture; Studies on Coix Ma-yuen Roman. J Korean Pharm Sci 5: 7-11
  2. Park YJ, Lee YS, Suzuki H. 1988. Effect of Coix on plasma cholesterol and lipid metabolism in rats. Korean J Nutr 21: 88-98
  3. Park YK, Kang BS. 2000. Studies on the effects of Coicis Semen and Sophorae Radix on the antioxidation. Kor J herbology 15: 57-67
  4. Kim JK, Lee HS. 2000. Tyrosinase-inhibitory and radical scavenging activities from the seeds of Coix lachrvma-jobi L. var. ma-yuen [Roman.] Stapf. Korean J Food Sci Technol 32: 1409-1413
  5. Woo JW. 1991. Electrophoretic characterization of job's tears (Yulmoo: Coix laduvma-jobi L. var. Ma-yuen stapf. & Yeom-joo: Coix lachryma-jobi L.) proteins. Korean J Soc Food Sci 7(3): 13-20
  6. Otsuka H, Hirai Y, Nagao T, Yamasaki K. 1988. Anti-inflammatory activity of benzoxanoids from roots of Coix la-chryma-jobi L. var. ma-yuen. J Natural Products 51: 74-79 https://doi.org/10.1021/np50055a009
  7. Gomita Y, Ichimaru Y, Moriyama M, Fukamachi K, Uchikado A, Araki Y, Fukuda T, Koyama T. 1981. Behavioral and EEG effects of coixol (6-methoxy benzoxazolone), one of the components in Coix lachrvma-jobi L. var. ma-yuen Stapf. Folia Pharm Japan 77: 245-259 https://doi.org/10.1254/fpj.77.245
  8. Ukita T, Tanimura A. 1961. Studies on the antitumor components in the seeds of Coix lachryma-jobi L. var. ma-yuen Staph. I. Isolation and antitumor activity of coixenolide. Chem Pharm Bull 9: 43-46 https://doi.org/10.1248/cpb.9.43
  9. Tokuda H, Matsumoto T, Konoshima T, Kozuka M, Nishino H, Iwashima A. 1990. Inhibitory effects on Epstein-Barr virus activation and antitumor promoting activities of coix seed. Planta Med 56: 653-654
  10. Numata M, Yamamoto A, Moribayash A, Yamada H. 1994. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jobi. Planta Med 60: 356-359 https://doi.org/10.1055/s-2006-959500
  11. Takahashi M, Konno C, Hikino H. 1986. Isolation and hypoglycemic activity of Coixans A, Band C, glycans of Coix lachryma-iobi L. var. ma-vuen seeds. Planta Med 52: 64-65 https://doi.org/10.1055/s-2007-969074
  12. Shin DK, Park CH, Lee YJ, Lee YO. 1990. The effect of Job's tear diet on change of body lipid and tissue in rat. Kor J Oil Chem 7: 83-90
  13. Park J, Yang M, Jun HS, Lee JH, Bae HK, Park T. 2003. Effect of raw brown rice and Job's tear supplemented diet on serum and hepatic lipid concentrations, antioxidative system and immune function of rats. J Korean Soc Food Sci Nutr 32: 197-206 https://doi.org/10.3746/jkfn.2003.32.2.197
  14. Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS. 2004. Antioxidative and antimutagenic effects of Korean buckwheat, sorghum, millet and Job's tears. J Korean Soc Food Sci Nutr 33: 921-929 https://doi.org/10.3746/jkfn.2004.33.6.921
  15. Kim SE, Hwang BY, Kim YH, Kim YC, Lee KS, Lee JJ 1997. Multidrug -resistance reversing activity of medicinal plants. Kor J Pharmacogn 28: 174-178
  16. Lee EJ, Yang JS. 2002. Changes of free radical concentrations with irradiation dose and storage time in gamma-irradiated sesame and perilla seeds. Korean J Food Sci Technol 34: 396-399
  17. Byun MW. 1994. Application of irradiation techniques to food industry. Radioisotope News 9: 32-37
  18. Lim SI, Yuk HS, Yoon HH, Kim YJ, Byun MW. 1998. Effect of gamma irradiation on egg white protein. J Korean Soc Food Sci Nutr 27: 291-295
  19. Farag RS, Khawas KH. 1998. Influence of $\gamma$-irradiation and microwaves on the antioxidant property of some essential oils. Inter J Food Sci Nutr 49: 109-115 https://doi.org/10.3109/09637489809089390
  20. Thayer DW. 1990. Food irradiation: Benefits and concerns. J Food Quality 13: 147-169 https://doi.org/10.1111/j.1745-4557.1990.tb00014.x
  21. Son JH, Jo C, Kim MR, Kim JO, Byun MW. 2001. Effect of gamma irradiation on removal of undesirable color from green tea extracts. J Korean Soc Food Sci Nutr 30: 1305-1308
  22. Pieters R, Huismans DR, Leyva A, Veerman AJP. 1998. Adaptation of the rapid automatic tetrazolium dye based (MTT) assay for chemosensitivity testing in childhood leukemia. Cancer Letters 41: 323-332 https://doi.org/10.1016/0304-3835(88)90294-7
  23. Fischer SM, Ley ton LJ, Lee ML, Locnislcar M, Belury MA, Maldve RE. 1992. Differential effects of dietary linoleic acid on mouse skin-tumor promotion and mammary carcinogenesis. Cancer Res (Suppl.) 52: 2049-2056
  24. Lee SW, Hwang BY, Kim SE, Kim HM, Kim YH, Lee KS, Lee JJ, Ro JS. 1995. Isolation of modulators for multidrug resistance from the fruits of Evodia officinalis. Kor J Pharmacogn 26: 344-348
  25. Ha SC. 1996. Reversal activity of multidrug-resistance by hatomarubigins. Kor J Appl Microbiol Biotechnol 24: 242-246
  26. Hwang EJ, Cha YJ, Park MH, Lee JW, Lee SY. 2004. Cytotoxicity and chemosensitizing effect of camellia (Camellia japonica) tea extracts. J Korean Soc Food Sci Nutr 33: 487-493 https://doi.org/10.3746/jkfn.2004.33.3.487

Cited by

  1. Antioxidant Compounds and Activities of Foxtail Millet, Proso Millet and Sorghum with Different Pulverizing Methods vol.40, pp.6, 2011, https://doi.org/10.3746/jkfn.2011.40.6.790
  2. Physicochemical Characteristics of Vinegars Fermented from Cereal Crops with Incalgyun vol.39, pp.8, 2010, https://doi.org/10.3746/jkfn.2010.39.8.1171
  3. The quality Characteristics of Saccharified Minor Cereal Gruel Prepared with Different Grain Kojis vol.27, pp.6, 2011, https://doi.org/10.9724/kfcs.2011.27.6.661
  4. Endoplasmic reticulum stress induced by an ethanol extract of Coicis semen in Chang liver cells vol.18, pp.1, 2018, https://doi.org/10.1186/s12906-018-2175-z