Effect of Dietary Iron and Exercise on Heme Biosynthesis and Lipid Metabolism of Rats

철분과 운동이 흰쥐의 헴 생합성과 지질 대사에 미치는 영향

  • 최지영 (용인대학교 식품영양학과) ;
  • 김혜영 (용인대학교 식품영양학과)
  • Published : 2005.06.01


This study was peformed to investigate the effect of dietary iron and exercise on heme biosynthesis and lipid metabolism of rats. Rats were divided into 4 groups according to dietary iron supplement (Fe-,5 ppm vs. Fe+, 50 ppm) and exercise status (control us exercise). The experiment continued for 8 weeks. Regular treadmill exercise ($6^{\circ}$, 28 m/min, 30 min duration, 5 days/week) was given to exercise groups. The aminolevulinate dehydrase activity of red blood cell, the marker of heme biosynthesis, was significantly increased in the exercise group. Regular aerobic exercise reduced body weight and epidydimal fat pad gain. Regular exercise also significantly decreased the plasma triglyceride, cholesterol and HDL-cholesterol of the rats. Meanwhile, the iron deficiency decreased the hematocrit and hemoglobin concentration and increased the accumulation of liver cholesterol.


  1. Abraham WM, Terjung RL. 1978. Increased $\delta$-aminolevulinic acid synthetase activity in rat ventricle after acute exercise. J Appl Physiol 44: 507-511
  2. Beattie DS. 1971. The possible relationship between heme synthesis and mitochondrial biogeness. Arch Biochem Environ Biophys 147: 136-142
  3. Matsuo T, Mizushima Y, Fujie M, Suzuki M. 1999. Effect of voluntary resistance exercise on mitochondrial heme biosynthesis in rat liver. J Clin Biochem Nutr 27: 151-159
  4. Matsuo T, Kang SH, Suzuki H, Suzuki M. 2002. Voluntary resistance exercise improves blood hemoglobin concentration in severely iron-deficient rats. J Nutr Sci Vitaminol 48: 161-164
  5. Granick S. 1966. The induction in vitro of the synthesis of $\delta$-aminolevulinic acid synthetase in chemical porphyria: a response to certain drugs, sex hormones and foreign chemicals. J Biol Chem 241: 1359-1375
  6. Granick S, Urata G. 1963. Increase in activity of $\delta$-aminolevulinic acid synthetase in liver mitochondria induced by feeding of 3,5-dicarbethoxy-l,4 dihydrocollidine. J Biol Chem 238: 821 -827
  7. Weaver CM, Rajaram S. 1992. Exercise and iron status. J Nutr 122: 782-787
  8. Scjokman CP, Ingrid H, Rutishauser E, Wallace RJ. 1999. Pre-and post-game macronutrient intake of a group of elite Australian football players. Int J Sport Nutr Exerc Metab 9: 60-96
  9. Foster C, Thompon NN, Dean J, Kirkendall DT. 1986. Carbohydrate supplementation and performance in soccer players. Med Sci Sports Exerc 18(suppl): S12
  10. Boggs TR, Morris RS. 1909. Experimental lipemia in rabbits. J Exp Med 11: 553-560
  11. Weight LM, Jacobs P, Noakes TD. 1992. Dietary iron deficiency and sports anaemia. Br J Nutr 68: 253-260
  12. Finch CA, Cook JD. 1984. Iron deficiency. Am J Clin Nutr 39: 471-477
  13. Kim HYP, Lee PY, Jang YA. 1998. Iron status of female athletes involved in aerobic sports. Nutr Sci 1: 29-33
  14. Sassa S. 1982. $\delta$-amiolevulinic acid dehydratase assay. Enzyme 28: 133-145
  15. Chan SK, Reibling A, Mahaffey WL, Lin CC. 1973. The $\delta$-aminolevulinic acid synthetase activity and the effect of exogenous $\delta$-aminolevulinate on the synthesis of cytochrome c in the thoracic muscles of the tobacco horn worm during adult development. Biochem Biophys Acta 329: 251-255
  16. Granick S, Sassa S. 1971. $\delta$-aminolevulinic acid synthetase and the control of heme and chlorophyll synthesis. In Metabolic regulation. Vogel HJ, ed. Academic, New York. Vol 5, p 77-141
  17. Prasad MK, Pratt CA. 1990. The effects of exercise and two levels of dietary iron on iron status. Nutr Res 10: 1273-1283
  18. Holloszy JO, William WW. 1979. Induction of $\delta$-aminolevulinic acid synthase in muscle by exercise or thyroxine. Am J Physiol 236: R180-R183
  19. Zhong MQ, De SX, Qin KL, Kwok PH. 2001. Effect of different duratoins of exercise on transferrin-bound iron uptake by rat erythroblast. J Nutr Biochem 13: 47-54
  20. Kang HS. 2002. Effect of long-term voluntary resistance exercise on heme biosynthesis. Proceedings of 2004 Fall Korean Nutritional Science Meeting. p 89
  21. Roughead ZK, Johnson LK, Hunt JR. 1999. Dietary copper primarily affects antioxidant capacity and dietary iron mainly affects iron status in a surface response study of female rats fed varying concentration of iron, zinc and copper. J Nutr 129: 1368-1376
  22. Kim HYP, Kang HS. 2003. Effect of iron supplementation and training on serum lipid and lipoprotein cholesterol profile. Korean J Environ Biology 21: 189-193
  23. Chung IK. 1986. The changes of blood iron, lipid and exercise capacity during summer training period. MS Thesis. Korea Univ., Korea