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Effect of Carthami Tinctorii Fructus Herbal-acupuncture Solution(CTF-HAS) on Gene Expression in SNU484 carcinomar cells

Oligonucleotide chip을 이용한 홍화자약침액(紅花子藥鍼液)이 위암세포주(胃癌細胞柱)의 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響)

  • Lee, Kyung-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Lim, Seong-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Han, Sang-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University)
  • 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 한상원 (대구한의대학교 한의과대학 침구경혈학교실)
  • Published : 2005.02.28

Abstract

Objectives : It has long been known about the osteogenic effect of CTF-HAS on bone tissues. However, it has not been determined the effect of CTF-HAS on cancer cells. The purpose of this study is to screen the CTF-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approach were employed to screen the differential expression genes. Methods : CTF-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of CTF-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cytotoxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of CTF-HAS. For oligonucleotide microarry assay, total RNA was used for gene expression analysis using oligonucleotide genechip (Human genome U133 Plus 2.0., Affimatrix Co.). Results : It has no cytotoxic effects on HepG2 cells in all concentration (0.1, 0.5, 1.5, 10,20mg/ml). More than twofold up-regulated genes were 5 genes. The number of more than twofold down-regulated genes was 10. Discussion : This study showed the screening of CTF-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray. The screened genes will be used for the better understanding in therapeutic effect of CTF-HAS on cancer field.

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