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Experimental Study on Anti-body effects of Anti-BV on the Bee Venom Herbal Acupuncture

Anti-BV의 봉약침 항체 효능에 관한 실험적 연구

  • Kwon, Ki-Rok (Department of Acupuncture & Moxibution, College of Oriental Medicine, Sang-ji University) ;
  • Lee, Kwang-Ho (Department of Acupuncture & Moxibution, College of Oriental Medicine, Sang-ji University) ;
  • Park, Won-Pil (Department of Acupuncture & Moxibution, College of Oriental Medicine, Sang-ji University)
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 이광호 (상지대학교 한의과대학 침구학교실) ;
  • 박원필 (상지대학교 한의과대학 침구학교실)
  • Published : 2005.02.28

Abstract

Objectives : To observe physiological anti-body effects of anti-BV, acute toxic response, measurement of $LD_{50}$, and the effects of anti-body were evaluated. Methods : $LD_{50}$ of Anti-Bee Venom were measured, and to analyze acute toxic responses, weight, and the anti-body effects various concentrations of Anti-BV were diluted and the survival rate was measured. Cell blood count (CBC), liver, spleen, and kidney pathologies were observed from the histological aspects. Results : Experiment was conducted to observe Anti-BV as the anti-body to the bee venom and the following results were obtained : 1.anti-BV was injected intraperitoneally and no toxic responses were witnessed. All of the experiment subjects stayed alive during the experiment, making $LD_{50}$ analysis impossible. 2.Anti-BV was injected intraperitoneally in mice and no significant weight changes were measured between the control group and the experiment groups. 3. Measuring the concentration dependent survival rate, the highest survival rate was at the concentration of $1.25{\times}10^2mg/kg$(1/2.000) for Anti-BV. 4. No particular results were shown in the CBC test. 5. Observation of changes in the organ tissues, Anti-BV was found to suppress blood stasis in the liver and inhibit necrosis of the cells. Conclusion : Above results suggest that Anti-BV doesn't cause any toxic responses in the body and works as an anti-body to the bee venom. Further studies must be followed to secure the findings.

References

  1. 권기록. 蜂針에 대한 考察. 대한 침구학회지 Vol 11. No1, 160, 1994
  2. 이성노 외, 봉약침 치료의 퇴행성 슬관절염에 대한 임상적 고찰. 대한 침구학회지 20권 5 호, 73-81, 2003.
  3. 권기록. 봉독요법의 류마티스성 관절염 치료에 대한 임상적 연구. 전국한의학 학술대회지 1998:130–131.
  4. 이상훈 외, 무작위 대조 이중맹검 시험을 통한 봉독약침의 류마티스 관절염 치료효과. 대한침구학회지 20권 6호, 80-87, 2003.
  5. Schmidt J. O, Allergy to hymenoptera venoms: in Piek T. ed, Venoms of the hymenoptera. London: Academic press; 510, 1986.
  6. 권기록, 봉독요법의 면역반응에 관한 임상적 연구. 대한침구학회지, Vol 17, No.1, 169-174, 1999.
  7. 황태준 외, 봉독의 항독소 생산을 위한 실험적 연구. 대한약침학회지 Vol. 4, No. 2, 5-16, 2001. https://doi.org/10.3831/KPI.2001.4.2.005
  8. Akita E. M. and Nakai, S., Immunoglobulin from egg yolk; isolation and purification. J. Food Sci. 57, 629. 1992. https://doi.org/10.1111/j.1365-2621.1992.tb08058.x
  9. 식품의약품안전본부. '의약품 독성시험기준' 제98-116호.
  10. 권기록. 한방치료를 통한 근위축성 측삭경화증의 임상적 연구. 대한침구학회지 20권 3호, 209-216, 2003.
  11. 강계성 외, 다발성 경화증 환자 5례에 대한 임상보고. 대한침구학회지 20권 1호, 209-217, 2003.
  12. Matsuda, T., Kawauchi, M. and Nakamura, R.: Growth of ovarian follicle and yolk protein accumulation. japanese J. Dairy & Food Sci. 41(6), 223, 1992.
  13. Gutierrez M. A., Miyazaki T., hatta H, Kim M.. Protective properties of egg yolk IgY containing anti-Edwardsiella tarda antibody against paracolo disease in the Japanese eel, Anguilla japonica Temminck & Schlegel. J. Fish Dis., 16, 113–122. 1993. https://doi.org/10.1111/j.1365-2761.1993.tb00854.x
  14. 전형준 외, 봉약침으로 치료한 요추간판탈출증 환자의 임상적 평가. 대한침구학회지 20권 5호, 63-72, 2003.

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