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Effects of distilled Phaseoli Semen rubra Herbal-Acupuncture on lipid composition, liver function, antioxidant capacity and molecular biological aspects in obese rats induced high fat diet

적소두증유약침(赤小豆蒸溜藥鍼)이 비만(肥滿)쥐의 지질구성(脂質構成), 간장기능(肝臟機能), 항산화효과(抗酸化效果) 및 분자생물학적(分子生物學的) 특성(特性)에 미치는 영향(影響)

  • Lee, Jun-Mu (Department. of Oriental Medicine, Sangji University) ;
  • Ji, Jun-Hwan (Department. of Oriental Medicine, Sangji University)
  • 이준무 (상지대학교 한의과대학 경혈학교실) ;
  • 지준환 (상지대학교 한의과대학 경혈학교실)
  • Published : 2005.06.30

Abstract

Effects of Phaseoli Semen rubra Herbal-acupuncture at zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) on lipid composition, liver function, oxidative capacity and molecular biological aspects were investigate in high fat diet induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups according to body weight and raised four weeks with control, zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) Herbal-acupuncture groups. 1. Plasma total cholesterol and LDL-cholesterol showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups and HDL-cholesterol showed a high values in the (ST-36) Herbal-acupuncture groups. 2. Plasma triglyceride and glucose showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups. 3. The contents of plasma free fatty acids showed a tendence to decrease in the Herbal-acupuncture groups, however in the Herbal-acupuncture groups, the values showed no significantly different. 4. The activities of AST and ALT showed no significantly different in all treatment groups. 5. The contents of plasma ${\beta}-lipoprotein$ and free fatty acids showed a tendency to decrease in the Herbal-acupuncture groups compared to those of control group. In the Herbal-acupuncture groups, the values of (ST-36) and (LI-11) Herbal-acupuncture groups showed a low in the acupuncture groups. 6. Liver total cholesterol and triglyceride showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups. 7. Contents of plasma TBARS showed a low values in the (ST-36) and (LI-11) Herbal-acupuncture groups, however contents of liver TBARS showed no significantly different among treatment groups. 8. Values of liver glutathione peroxidase and catalase activity showed a tendency to increase in the (ST-36) and (LI-11) Herbal­acupuncture groups. Values of liver super oxide dismutase activity showed a high in the (ST-36) Herbal-acupuncture groups compared to those of other groups. 9. Expression of apo-B mRNA in liver cell showed a low in (ST-36) Herbal-acupuncture groups, however expression of apo-E mRNA showed a high in Herbal-acupuncture groups and $TNF-{\alpha}$ mRNA in adipose cell showed no different among all the treatment groups. Expression of Leptin mRNA showed a tendance to decrease in (ST-36) Herbal-acupuncture group. 10. Histological character of liver, those of (ST-36) Herbal-acupuncture group showed a good, however other treatment groups showed slight vasodilation and slight fat accumulation. These results indicated that Phaseoli Semen rubra Herbal-acupuncture at (ST-36) and (LI-22) suppressed adipose tissue mass and lipid peroxidation and increased antioxidant system.

Keywords

References

  1. Barker J, Liu JP, Robertson EJ, Estratiadis A. Role of insulin-like growth factors in embryonic and postnatal growth. Cell. 75(1):73-82, 1993 https://doi.org/10.1016/S0092-8674(05)80085-6
  2. Hill JO, Lin D, Yakybu F, Peters JC. Development of dietary dbesity in rats : influence of amount and composition of dietary fat. Int. J. Obes. Relat. Metab. Disord. 16(5):321-333, 1992.
  3. Weigle DS. Appetite and the regulation of body composition. FASEB. J. 8(3):302-310, 1994.
  4. Nielsen S, Jensen MD. Obesity and cardivascular disease is body structure a factor. Curr. Opin. Lipidol. 8(4):200-204, 1997. https://doi.org/10.1097/00041433-199708000-00002
  5. Kissebah AH, Alfarsi S, Adams PW, Wynn V. Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man. Diabetologia. 12(6):563-571, 1976. https://doi.org/10.1007/BF01220632
  6. Lee KU, Lee HK, Koh CS, Min HK. Artificial induction of intravascular lipolysis by lipid-heparin infusion leads to insulin resistance in man. Diabetologia. 31(5):285-290, 1988. https://doi.org/10.1007/BF00277409
  7. Bray GA. Obesity increase risk for diabetes. Int. J. Obes. Relat. Metab. Disord.16(Suppl) 4:S13-S17, 1992.
  8. Graham S. Marshall J. Haughey B. Mittelman A. Swanson M. Zielezeny M. Byers T. Wilkinson G. West D. Dietary epidemiology of cancer of the colon in western New York. Am. J. Epidemiol. 128(3):490-503, 1988.
  9. Jain M, Cook GM, Davis FG, Grace MG, Howe GR, Miller AB. A case-control study of diet and colo-rectal cancer. Int. J. Cancer. 26(6):757-768, 1980. https://doi.org/10.1002/ijc.2910260609
  10. Van der HB. Bar PR. Kapplle LJ. Wildt DJ. Dietary vitamin E levels affect outcome of permanent focal cerebral ischemia in rat. Stroke. 29(5):1002-1006, 1998. https://doi.org/10.1161/01.STR.29.5.1002
  11. Yang SQ. Lin HZ. Lane MD. Clemens M. Diehi AM. Obesity increase sensitivity to endotoin liver injury: implications for the pathogenesis of steatohepatitis. Proc. Natl. Acad. Sci. USA. 94(6):2557-2562, 1997. https://doi.org/10.1073/pnas.94.6.2557
  12. Ronald M. Krauss. Mary W. Obesity : Impact on cardiovascular disease. Circulation. 98:1472-1476, 1998. https://doi.org/10.1161/01.CIR.98.14.1472
  13. Robert HE. Ronald M. Krauss. American heart association call to action : Obesity as a major risk factor for coronary heart disease. Circulation. 97:2099-2100, 1998. https://doi.org/10.1161/01.CIR.97.21.2099
  14. William HD. Childhood weight after adult morbidity and mortality. J. Nutr. 128:411S-414S, 1998.
  15. 吳武. 按摩治療肥感證21例效果觀察, 按摩與圖, 1, 1988.
  16. 허준著. 東醫寶鑑 남산당 73, 1987.
  17. 申署謹. 600例 單純性肥滿患者與中醫分型的關係, 第2次全國中西醫結合肥滿病硏究學術論文的要偏 10, 1989.
  18. 李南勳, 李京燮. 防風通聖散이 高血壓,고지혈에 미치는 영향. 대한한의학회지, 12(1), 44-55, 1991.
  19. 金珍泰, 靑雲化痰湯이 지질대사에 미치는 영향. 경희한의대논문집, 7, 239-247, 1984.
  20. 盧承鉉. 凉膈散이 血壓 및 지혈에 미치는 영향. 경희한의대논문집. 8, 219-228, 1985.
  21. 經京河. 生肝湯이 高脂血症에 미치는 영향. 경희한의대논문집. 12, 263-283, 1989.
  22. 劉抗華. 耳穴坤壓治療肥滿症 567例臨床觀察, 上海鍼灸雜誌, 4, 1986
  23. 王聲芳. 耳壓治療肥滿症50例小結. 四川中醫, 5, 4, 1987.
  24. 張忠志. 耳針對靑年男女體型的影響. 中國鍼灸, 10, 5, 1989.
  25. 趙銀龍. 鍼灸對單純性肥滿患者糖代謝的影響. 第2次全國中西醫結合肥滿病硏究學術論文的要編, 7, 1989.
  26. 劉志誠. 鍼灸對單純肥滿病發高血壓患者的調整作用. 第2次全國中西醫結合肥滿病硏究學術論文的要編, 17, 1989.
  27. 李凝凜. 針刺減肥300例療效分析. 上海鍼灸雜誌. 7, 3, 1988.
  28. 原桃仁. 肥滿的中醫治療. 第2次中國中西醫結合肥滿病硏究學術論文要的編, 7, 1989.
  29. 江幼李. 肥滿的中醫治療. 北京中醫學院報, 8, 2, 1985.
  30. 王玉芬. 感肥驗案4則, 中醫雜誌. 27, 11, 1986.
  31. 康延培. 經身飮II號治療單純性肥滿病46例臨床療效觀察, 中醫雜誌. 28, 12, 1987.
  32. 李降中. "體可輕" 治療肥滿病臨床觀察. 2, 10, 1986.
  33. 노성규, 강성길. 防風수침이 高血壓 및 高脂血症에 미치는 영향. 경희한의대 논문집, 8, 157-170, 1985.
  34. 이호섭, 변재영, 유윤조. 약침이 자발성 고혈압 백서의 혈압에 미치는 영향, 대한침구학회지, 13(1), 422-428, 1996.
  35. 박진웅, 고형균, 김창환. 농도차에 따른 황기약침이 Methotrexate를 투여한 생쥐의 면역반응에 미치는 영향. 대한침구학회지, 11(1), 67-81, 1994.
  36. 안현석, 박영배, 강성길. 현호삭 약침자극이 진통, 항마비 및 항괴양 효과에 미치는 영향. 대한침구학회지, 11(1), 99-111, 1994.
  37. 성일환, 채우석, 현호삭, 당귀미 수침이 Endotoxin으로 유발된 백서의 혈전증에 미치는 영향. 대한침구학회지, 11(1), 391-404, 1994.
  38. 이준무. 간수 및 일월 청호약침이 고지방 급여 흰쥐의 혈청지질구성에 미치는 영향. 대한경락경혈학회지, 20(3), 91-99, 2003.
  39. 辛民敎 臨床本草學 南山堂, 600-601, 1986.
  40. 林鍾國, 鍼灸治療學 集文堂, 278, 304-305, 317, 1983.
  41. 林鍾國. 艾久生體反應의 文獻的考察, 東洋醫學. 13, pp.63-68, 1976.
  42. Buge, J.A. Austa, S.D. Microsomal lipid peroxidation, 1978, 52, pp. 302-309. In : Methods in Enzymology. Fleicher, S., Packer, L. (eds). Academic Press, London, 1978.
  43. Levander, O.A., Deloach, D.P., Morris, V.C., Moser, P.B. Platelet glutathione peroxidase activity as an index of selenium status in rats. J. Nutr, p.113, pp.55-63, 1983.
  44. Flohe L, Becker R, Brigelius R, Lengfelder E. Otting F. Convenient as says for superoxide dismutase. CRC Handbook of free radicals and antioxidants in Biomedicine pp.287-293, 1992.
  45. Johnson LH, Hakan Borg LA. A spectrophtometric method for determination of catalase activity in small tissue samples. Analytical Biochemistry 1988:174, 331-336, 1988. https://doi.org/10.1016/0003-2697(88)90554-4
  46. 崔述貴. 耳針治療肥滿550例療效分析, 中醫藥新式. 5, 1986.
  47. Dahlqvist A., Johnsen J. Awarning against ear acupuncture used in the treatment of obesity. Lakartidningen. Sep. 16,84-38,1987.
  48. MV. Bogdanovich VL. Role of reflexotherapy in the complex, treatment of neuroendocrine obesity. TerArkh, 57, 10, 97-100, 1985.
  49. 김은성, 김미경. 감잎, 녹차, 솔잎의 건분 및 에타놀추출물이 흰쥐의 지방대사와 항산화능에 미치는 영향. 한국영양학회지,1999;32(4),337-352, 1999.
  50. 이은, 최무영. 솔잎분말이고콜레스테롤급여 흰쥐의 체지질구성과 TBARS량에 미치는 영향. 한국식품과학회지, 32(5), 1186-1190, 2000.
  51. Lee,E. Effects of Green tea on serum lipid composition and TBARS in chicks with oxidized lipid. Sangji Univ. Life Sci. Research Ins. 51-56, 1998.
  52. 原桃仁. 肥滿的中醫治療. 第2次中國中西醫結合肥滿病硏究學術論文要的編, 7, 1989.
  53. 王秀風. 中風治療單純性肥滿44例療效觀察, 中醫雜誌, 1, 1988.
  54. 趙銀龍. 鍼灸對單純性肥滿患者糖代謝的影響. 第2次全國中西醫結合肥滿病硏究學術論文的要編, 7, 1989.
  55. 孫廣培. 耳穴點壓加防風通聖丸治療肥滿症147例, 四川中醫, 6, 2, 1988.
  56. 陳瑞榮. 鍼灸配合治療單純性肥滿95例臨床觀察. 中醫雜誌, 7, 1987.
  57. 宗文九. 痰飮淺說. 上海, 上海科技, 51-52, 1985.
  58. 安一, 李東熙. 淸心湯이 실험적 가토 高脂血症에 미치는 영향에 관한 硏究. 경희한의대 논문집, 2, 131-146, 1982.
  59. 宋孝貞, 文濬典. 淸上瀉火湯이 혈압 및 지질대사에 미치는 영향. 경희한의대논문집, 2, 127-134, 1979.
  60. 孫彰奎. 淸肝湯이 高脂血症에 미치는 영향. 대전대학교석사학위논문. 1991.
  61. 權寧哲, 李京燮. 疎風湯 및 加味疎風湯이 高脂血症에 미치는 영향. 경희한의대논문집, 5,269-279, 1982.
  62. 朴鍾榮, 李京燮. 祛風續命湯이 지질대사에 미치는 영향에 관한 硏究. 경희대한의대논문집, 5, 335-343, 1982.
  63. 崔淳暾, 半夏白朮天麻湯이 高脂血症에 미치는 영향.대전대학교 碩士學位논문, 1990.
  64. 李大植. 高血壓 및 高脂血症에 대한 淸熱導痰湯의 實驗的 硏究. 大韓韓方內科學會紙, 12, 16-23, 1991.
  65. 陳湘君. 人蔘降脂合濟的臨床及動物實驗硏究. 遼寧中醫雜誌, 12(1), 21-23, 1988.
  66. 朱良爭. 通瘀靈治療糖尿病高脂血症的臨床化學實驗硏究. 中醫雜誌, 32(12), 23-25, 1991.
  67. 洪元植, 黃帝內經素問, 서울, 集文堂, p164,361, 1985.
  68. 朴元煥. 血栓症과 打撲性 充血 및 高脂血症에 順氣導痰湯 및 化瘀湯이 미치는 영향. 동국대학교 博士學位논문, 1992.
  69. 崔承勳. 血府遂瘀湯이 血栓症과 皮下血腫에 미치는 影響. 대한동의병리학회지, 2, 26-47, 1987.
  70. 崔承勳. 黃帝內經에서의 瘀血의 認識에 대한 理論的 硏究. 대한동의병리학회지, 3, 12-17, 1988.
  71. 呂明壓. 耳穴按壓法感肥 1000例臨床療效報道. 貴州醫藥, 10(5), 1986.
  72. 殷榮亮. 耳穴點壓治療肥滿症200例療 觀察. 協書中醫. 7, 5, 1986.
  73. 李士杰. 耳穴坤鍼感肥253例臨床觀察, 中國鍼灸, 6, 3, 1986.
  74. Ishikawa T, Suzukawa M. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. Am Clin Nutr. , 66(2), pp.261-266. 1997.
  75. Ueda, H. and Tanoue K. Growth-depressing and cholesterol-lowering effects of quillaja and tea saponins in chicks as influenced by diet composition. Anim. Sci. J. 71, (4), pp.393-399. 2000.
  76. 김종호. 臨床生化學, 高麗醫學. 1998.
  77. Anderson JW. The nutritonal therapy of non-insulin dependent (type II) diabetes, Diabetes Educator. p.9, pp.13-18. 1983. https://doi.org/10.1177/014572178300900303
  78. 조수열, 박미리, 박원학, 정재홍. 콜레스테롤식이로 사육한 흰쥐의 혈장 및 간지질성분에 미치는 카페인 및 비타민 E의 영향. 한국영양식량학회지. 18(1),pp.53-61. 1989.

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