DOI QR코드

DOI QR Code

Fluorescent Method for Observing Intravascular Bonghan Duct

형광염색을 이용한 혈관내봉한관의 관찰

  • Lee, Byung-Cheon (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Baik, Ku-Youn (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Johng, Hyeon-Min (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Sung, Baeck-Kyoung (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Soh, Kyung-Soon (College of Oriental medicine, Semyung University) ;
  • Kang, Dae-In (College of Oriental medicine, Semyung University) ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory, School of Physics, Seoul National University)
  • 이병천 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 백구연 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 정현민 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 성백경 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 소경순 (세명대학교 한의과대학) ;
  • 강대인 (세명대학교 한의과대학) ;
  • 소광섭 (서울대학교 자연과학대학 물리학부 한의학물리연구실)
  • Published : 2005.12.30

Abstract

Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.

References

  1. Kim, B. H.(1963). On the Kyungrak System. Medical Science Press Pyongyang.
  2. Jiang, X., Kim, H. K., Shin, H. S., Lee, B. C., Choi, C. H., Cheun, B. S., Baik, K. Y, and Soh, K. S. (2002) Method for Observing Intravascular Bonghan Duct. Kor. J. Orient. Prev. Med. 6:162–166.
  3. Jiang X, Lee B.C. Choi C. et al., (2004) Tubular substructure of intravascular threadlike structure from rats and rabbits. J. Kor. Phys. Soc. 44:1602–1604.
  4. Shin, H. S., Soh, K. S. (2002) Electrical Method to Detect a Bonghan Duct Inside Blood Vessels. New Physics (SaeMulli). 45:376–378.(in Korean)
  5. Baik KY, Lee J, Lee BC, et al. (2004) Acupuncture Meridian and Intravascular Bonghan Duct. Key Eng. Mat., accepted https://doi.org/10.4028/www.scientific.net/KEM.277-279.125
  6. Baik, K. Y., Park, E. S., Lee, B. C., Kim, H. K., Cho, S. I., Jiang, X, Shin, H. S., Choi, C. H., Yi, S. H., Johng H. M., Nam, T. J., Soh, K. S., Cheun, B. S., Park, D. H., Nahm, Y. S., Yoon, Y. S., Lee, I. S., Ahn, S. Y., and Soh, K. S., (2004) Histology of threadlike structure inside blood vessel. J. Intl. Soc. Life Info. Sci. 22, to be published.
  7. Lee BC, Baik KY, Johng HM, et al. (2004) Acridine orange staining method to reveal characteristic features of intravascular threadlike structure. Anat. Rec. B. 278B:27–30. https://doi.org/10.1002/ar.b.20018
  8. Baik KY, Lee J, Lee BC, Johng HM, Nam TJ, Sung B, Soh KS, (2004) Long Threadlike Structures inside the Blood Vessels of Rats, New. Med. J., 47(7), 18–22
  9. Lee BC, Baik KY, Cho S, Min C, Johng HM, Hahm J, Choi C, Yi SH, Soh KS, Park DH, Ahn SY, Cheon BS, Soh KS, (2003) Comparison of Intravascular Bonghan Ducts from Rats and Mice, Kor. J. Orient. Prev. Med. 7:47–53.