Pharmacognostical Evaluation of Leucas aspera Link.

  • Rai, Vartika (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Agarwal, Manisha (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Agnihotri, A.K. (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Khatoon, S. (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Rawat, A.K.S. (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Mehrotra, S. (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute)
  • Published : 2005.06.01


Leucas aspera Link. (Lamiaceae) is an important medicinal plant in indigenous systems of medicine in India and commonly known as 'Thumbai'. It has various ethnomedicinal values as various traditional communities find diverse medicinal properties. It is used as antipyretic, stimulant, expectorant and diaphoretic drug. The present communication deals with the detailed pharmacognostical evaluation of whole plant of L. aspera collected from five different geographical zones of the country-Uttar Pradesh, Orissa, Karnataka, West Bengal and Gujarat. The botanical characters and TLC fingerprint profile of all the samples were quite similar but some variations were observed in physicochemical parameters. However, some microscopical characters and TLC profile can be used as diagnostic characters for identification of L. aspera, for example amphistomachic leaves and two types of trichomes-abundant, non glandular, uniseriate, 1-3 celled and few glandular, 2-5 celled stalk with rounded tip. Presence of some components at $R_fs-0.56$, 0.65 and 0.76 under UV 366 and at $R_fs-0.31$, 0.43, 0.60, 0.76 and 0.82 under visible light after derivetization in TLC profile may also be used as diagnostic character.


  1. Anonymous, Official Methods of Analysis (AOAC), 14th Ed. Association of Official Analytical Chemists Inc. U.S.A. (1984)
  2. Sadhu, S.K., Okuyama, E., Fujimoto, H., and Ishibashi, M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chem. Pharm. Bull. 51, 595-598 (2003)
  3. Shivaprasad, B. and Chandrashekar, K.R. Plants used in the treatment of jaundice in Dakshina Kannada District. J. Econ. Tax. Bot. 27, 864-868 (2003)
  4. Srinivas, K., Rao, M.E.B. and Rao, S.S. Antiinflammatory activity of Heliotropium indicum Linn. and Leucas aspera Spreng. in albino rats. Indian J. Pharmacogn. 32, 37-38 (2000)
  5. Anonymous, Indian Pharmacopoeia, 2nd Edn. Government of India, New Delhi (1966)
  6. Saundane, A.R., Hidayatulla, K.M., and Satyanarayan, N.D., Antiinflammatory and analgesic activity of various extracts of Leucas aspera Spreng. (Labiatae). Indian J Pharm. Sci. 26, 144-146 (2000)
  7. Anonymoys, The Wealth of India, Vol. 6, CSIR Publication and Information Directorate, New Delhi (1962)
  8. Johansen, D.A., Plant Microtechniques 1st Ed. Mc Graw Hill Book Co. Inc. New York and London. pp. 182 (1940)
  9. Mont Gomery, R., Determination of glycogen. Arch. Biochem. Biophys. 67, 378-386 (1957)
  10. Kirtikar, K.R. and Basu, B.D. Indian Medicinal Plants, 2nd eds. Vol. IX Oriental Enterprises, Uttranchal, India (2001)
  11. Peach, K. and Tracy, M.V., Modern Methods of Plant Analysis, Vol. 3 & 4. Springer Verlag, Heidelberg (1955)
  12. Saxena, A.P. and Vyas, K.M. Ethnobotanical records on infectious disease from tribals of Banda district. J Econ. Tax. Bot. 2, 191-194 (1981)
  13. Kokoski, J., Kokoski, R. and Slama, F.J. Fluorescence of powdered vegetable drugs under ultraviolet radiation. J. Am. Pharm. 47, 75 (1958)
  14. Chase, C.R. and Pratt, R.J. Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. J. Amer. Pharm. Assoc. 38, 324-333 (1949)
  15. Shome, U. and Mehrotra, S. Pharmacognostic studies on 'Dronpushpi'. Ethnobotany 2, 105-115 (1990)
  16. Chopra, R.N., Nayar, S.L. and Chopra, I.C. Glossary of Indian medicinal Plants. CSIR, New Delhi (1956)