Activity Guided Isolation of Antioxidant Tannoid Principles from Anogeissus latifolia

  • Govindarajan, Raghavan (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Vijayakumar, Madhavan (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Shirwaikar, Annie (Pharmacognosy Department, College of Pharmaceutical Sciences) ;
  • Rawat, Ajay Kumar Singh (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Mehrotra, Shanta (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute) ;
  • Pushpangadan, Palpu (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute)
  • Published : 2005.09.01


Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.


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