EXPRESSION OF CYTOKERATIN SUBTYPES AND VIMENTIN IN AMELOBLASTOMA

사기질모세포종에서 Cytokeratin 아형과 Vimentin의 발현

  • Kang, Mi-Seon (Department of Pathology, Pusan Paik Hospital) ;
  • Yoon, Hye-Kyoung (Department of Pathology, Pusan Paik Hospital) ;
  • Kim, Woo-Hyung (Oral and Maxillofacial Surgery, Pusan Paik Hospital) ;
  • Choi, Soo-Im (Department of Pathology, Dongrae Paik Hospital, Inje University)
  • 강미선 (인제대학교 의과대학 부산백병원 병리과) ;
  • 윤혜경 (인제대학교 의과대학 부산백병원 병리과) ;
  • 김우형 (인제대학교 의과대학 부산백병원 구강외과) ;
  • 최수임 (동래백병원 병리과)
  • Published : 2005.08.31

Abstract

Ameloblastoma is the most common odontogenic tumor of the jawbones, but the origin of this tumor has been remained to be unproven. Cytokeratins (CKs) are specific intermediate filament of epithelial cells, and vimentin is expressed in mesenchymal cells. The immunohistochemical detection of different CKs and vimentin has made it easier to know the origin of tumor. Paraffin-embedded tissue sections from 15 ameloblastomas and 1 ameloblastic carcinoma were used for immunohistochemical evaluation of CK 7, 8, 13, 14, 19 and vimentin. Their expression is evaluated in different tumor cells, which are observed in different type of tumors. In the follicular and reticular subtype, central stellate cells of tumor nests expressed CK 8, 14, 19 and peripheral columnar cells expressed CK 14. CK 7, and 13 were not expressed. Vimentin was detected in fibrous stroma around tumor nest, not in tumor cells. The tumor cells of ameloblastic carcinoma expressed CK 7, 14 and 19, but CK 8 was more weakly stained than that in ameloblastoma. Central stellate cells and peripheral columnar cells of acanthomatous subtype showed same expression pattern with others. Meta plastic squamous cells expressed CK 8, 14, 19 and keratinizing squamous cells expressed CK 13, 19. CK 7 and vimentin were not detected in tumor cells and vimentin was expressed in fibrous stroma. Most of the tumor cells of ameloblastoma showed CK 14 and CK 19 and did not express CK 7 and vimentin. These findings were similar to the immunophenotype of dental lamina. And these results will be beneficial to differential diagnosis of odontogenic tumors and other kind of tumors arising at the oral cavity.

Keywords

References

  1. Kramer IR, Pinborg JJ, Shear M: The WHO histological typing of odontogenic tumors. A commentary on the second edition. Cancer 1992;70:2988-2994 https://doi.org/10.1002/1097-0142(19921215)70:12<2988::AID-CNCR2820701242>3.0.CO;2-V
  2. Sciubba JJ, Fantasia JA, Kahn LB: Atlas of tumor pathology-tumors and cysts of the jaw. Washington, DC., AFIP. 1999
  3. Benz Z. Pilch: Head and neck surgical pathology. Philadelphia, Lippincott Williams & Wilkins. 2001:208-215
  4. Waldron CA: Odontogenic cysts and tumours. In: Neville BW, Dam DD, Allen CM, Bouquot JE, editors. Oral and Maxillofacial pat hology. Philadelphia, WB Saunders. 2002:589-642
  5. Shafer WG: A texbook of oral pathology. 3rd ed. Philadelphia, W.B. Saunders. 1974
  6. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R: The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1092;31:11-24
  7. Quinlan RA, Schiller DL, Hatzfeld M, Achtstatter T, Moll R, Jorcano JL, et al: Patterns of expression and organization of cytokeratin intermediate filaments. Am NY Acad Sci 1985;455:282-306 https://doi.org/10.1111/j.1749-6632.1985.tb50418.x
  8. Crivelini MM, Araujo VC, Sousa SOM, Araujo NS: Cytokeratins in epithelia of odontogenic neoplasms. Oral Diseases 2003;9:1-6 https://doi.org/10.1034/j.1601-0825.2003.00861.x
  9. Gao Z, Mackenzie IC, Williams DM, Cruchley AT, Leigh I, Lane EB: Patterns of keratin-expression in rests of Malassez and periapical lesions. J Oral Pathol 1988;17:178-185 https://doi.org/10.1111/j.1600-0714.1988.tb01521.x
  10. Gao Z, Mackenzie IC, Cruchley AT, Williams DM, Leigh I, Lane EB: Cytokeratin expression of the odontogenic epithelia in dental follicles and developmental cysts. J Oral Pathol Med 1989;18:63-67 https://doi.org/10.1111/j.1600-0714.1989.tb00738.x
  11. Heikinheimo K, Hormia M, Stenman G, Virtanen I, Happonen RP:Patterns of expression of intermediate filaments in ameloblastoma and human fetal tooth germ. J Oral Pathol Med 1989;18:264-273 https://doi.org/10.1111/j.1600-0714.1989.tb00395.x
  12. Kasper M, Karsten U, Stosiek P, Moll R: Distribution of inermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin. Differentiation 1989;40: 207-214 https://doi.org/10.1111/j.1432-0436.1989.tb00600.x
  13. Pelissier A, Ouhayoun JP, Sawaf MH, Forest N: Evolution of cytokeratin expression in developing human tooth germ. J Biol Buccale 1990;18: 99-108
  14. Vigneswaran N, Whitaker SB, Budnick SD, Waldron CA: Expression patterns of epithelial differentation antigens and lectin-binding sites in ameloblastomas. A comparision with basal cell carcinmas Hum Pathol 1993;24: 49-57
  15. Matsuo A, Ueno S: Immunohistochemical demonstration of keratin in ameloblastoma as an indication of tumor differentiation. J Oral Maxillofac Surg 1991;49: 282-288 https://doi.org/10.1016/0278-2391(91)90221-7
  16. Ross MH, Kaye GI, Pawlina W: Histology. 4th ed. Philadelphia, Lippincott Williams & Wilkins. 2003 : 495-507
  17. Buchner A, Sciubba J: Peripheral epithelial odontogenic tumors:A review. Oral Surg Oral Med Oral Pathol 1987;63: 688-697 https://doi.org/10.1016/0030-4220(87)90372-0
  18. Simpson HE: Basal cell carcinoma and peripheral ameloblastoma. Oral Surg Oral Med Oral Pathol 1974;38: 233-2401 https://doi.org/10.1016/0030-4220(74)90062-0
  19. Martins MD, Cavalcanti de Araujo V, Raitz R, Soares de Araujo N:Expression of cytoskeletal proteins in developing human minor salivary glands. Eur J Oral Sci 2002;110:316-321
  20. Vigneswaran N, Peters KP, Hornstein OP, Haneke E: Comparison of cytokerain, filaggrin and involucrin profiles in oral leukoplakias and squamous carcinomas. J Oral Pathol Med 1989;18:377-390 https://doi.org/10.1111/j.1600-0714.1989.tb01569.x
  21. Cintorino M, Tripod SA, Santopietro R, Antonio P, Lutfi A, Chang F et al: Cytokeratin expression patterns as an indicator of tumor progression in oesophageal squamous cell carcinoma. Anticancer Res 2001;21(6A):4195-201