The Changes of Microflora During the Fermentation of Takju and Yakju

약.탁주 발효과정 중 미생물 균총의 변화

  • Published : 2005.02.28

Abstract

Korean traditional rice wine Takju and Yakju were manufactured using 2-step-brewing method. To investigate microflora involved in fermentation step, number of microorganisms, pH, titratable acidity, and alcohol contents of Takju and Yakju were measured. In Takju and Yakju, although not significantly, $1.1{\times}10^{8}$ and $2.0{\times}10^{6}\;CFU/mL$ lactic acid bacteria at initial stage of second fermentation decreased to $8.3{\times}10^{6}\;and\;1.0{\times}10^{4}\;CFU/mL$ at the end of second fermentation, respectively. For Takju, micrococci and yeast occupied 80 and 20% at initial stage of second fermentation, whereas bacteria and yeast occupied 35 and 65% at the end of second fermentation, respectively. Yeast occupied 88% throughout the second fermentation of Yakju. The main yeast isolated from both Takju and Yakju was identified as Saccharomyces cerevisiae using API 20C AUX kit. The yeast strain Candida magnoliae was also detected during fermentation of Takju and Yakju.

Keywords

Changes of microflora;Takju;Yakju;fermentation

References

  1. Song HI, Shin JY. Modern fermentation technology. p. 193 (1995)
  2. Kim CJ. Microbiological and enzymological studies on Takju brewing. J. Korean Agric. Chem. Soc. 10: 69-100 (1968)
  3. Jung JH, Jung ST. The changes of quality and micro flora during the preservation of Korean Takju. J. Korean Agric. Chem. Soc. 28: 252-260 (1985)
  4. Koh, CM, Choi, TJ, Lew, J. Microbiological studies on the Takju brewing: The Korean local wine. Korean J. Microbiol. 11: 167-174 (1973)
  5. Kim JO, Lee BH. Taxonomical studies of yeasts in Korea-On yeasts isolated from Takju. Korean J. Microbiol. 8: 77-84 (1970)
  6. Macfaddin JF. Biochemical test for identification of medical bacteria. 2nd ed. Wiliams and Wilkins (1980)
  7. Lee ZS, Rhee TW. Studies on the micro flora of Takju Brewing. Korean J. Microbiol. 8: 116-133 (1970)
  8. Shin KR, Kim BC, Yang JY, Kim YD. Characterization of Yakju prepared with yeasts from fruits. 1. Volatile components in Yakju during fermentation. J. Korean Soc. Food Sci. Nutr. 28: 794-800 (1999)
  9. KFDA. Code Food, Korea Food and Drug Association. Seoul, Korea (2003)
  10. Cappuccino JG, Sherman N. Microbiology, a laboratory manual. 2nd ed. Bejamin Commings. pp. 31-35 (1987)
  11. Han EH, Lee TS, Noh BS, Lee DS. Quality characteristics in mash of Takju prepared by using different Nuruk during fermentation. Korean J. Food Sci. Technol. 29: 555-562 (1997)
  12. Sneath PHA. Bergey's Manual of Systematic Bacteriology, Vol. 2. Wiliams and Wilkins (1986)
  13. Lee SR. Korean Fermentative Food. Ewha Women's University publishing department. p. 205 (1986)
  14. Technical Service Institute of National Tax Service. Manufacturing guideline of alcoholic beverages. pp. 83-176 (1997)
  15. Park YJ, Lee SK, Oh MJ. Studies on Takju yeasts. Part 1. Isolation and identification of Takju yeasts. J. Korean Agric. Chem. Soc. 16: 78-84 (1973)
  16. Shin YD, Cho DH. A study on the micro flora changes during Takju brewing. Korean J. Microbiol. 8: 53-64 (1970)
  17. Chung DH. Fermentation and microbial technology. Sunjin Munhwasa. pp. 228-275 (1974)