Antioxidative Effect of Crude Saponin Fraction Prepared from Culture Product of Basidiomycota cultured with Fresh Ginseng as Substrate

수삼을 기질로 한 담자균 배양물로부터 분리한 조사포닌의 항산화 효과

  • 정재현 (충주대학교 식품생명공학과) ;
  • 위재준 ((주)케이티앤지) ;
  • 신지영 (충청대학 다이어트건강관리학과) ;
  • 조주현 (에프이엔바이오텍(주)) ;
  • 정동현 (충주대학교 식품생명공학과)
  • Published : 2005.02.28

Abstract

Antioxidative activity of crude saponin fraction (CSF) prepared from Basidiomycota cultured with fresh ginseng (Panax ginseng C.A. Meyer) as substrate was investigated by analyzing CSFs for ginsenoside and phenolic compounds. On TLC chromatogram, ginsenosides such as $Rg_{2},\;Rg_{3}$, and $Rh_{1}$ which were rare in fresh ginseng, were identified. CSF of Phellinus linteus culture product showed the highest total phenolic content and electron donating ability (EDA), suggesting phenolic compounds contribute to EDA. In vitro lipid peroxidation was inhibited most by CSF of Ganoderma lucidum, indicating that the highest EDA does not imply highest inhibition against lipid peroxidation. Tyrosinase was also inhibited mostly by CSF of G. lucidum. These results suggest culture of Basidiomycota with fresh ginseng has more active substances than fresh ginseng alone.

Keywords

DPPH;Antioxidative;Fresh ginseng;Phellimus linteus;Gaooderma lucidum;Hericium erinaceum

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