Immunomodulatory Activities of Oat Bran Extracts with Different Extraction Conditions

추출조건에 따른 귀리 추출물의 면역활성

  • Park, Hee-Joeng (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Yun-Bae (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Tae-Su (Department of Food Science and Biotechnology, Chungbuk Provincial University) ;
  • Jung, Ick-Soo (Technical Research Institute, Bolak Company Limited) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2005.02.28

Abstract

Immunomodulatory activities of crude ${\beta}$-glucans extracted from oat bran under different conditions, fractions A ($55^{\circ}C,\;5%,\;pH\;6$), B ($45^{\circ}C,\;15%,\;pH\;6$), C ($50^{\circ}C,\;20%,\;pH\;7$), D ($50^{\circ}C,\;0%,\;pH\;7$), and E ($50^{\circ}C,\;10%,\;pH\;9$) were investigated. All crude ${\beta}$-glucan fractions stimulated macrophages, producing nitric oxide dose-dependently, and, efficiently promoted nitric oxide production in presence of IFN-${\gamma}$. Except for fraction C, in vivo test indicated fractions B, D, and E (100 mg/kg) substantially enhanced carbon-phagocytic indices of blood macrophages by oral administration of crude ${\beta}$glucan for 7 days prior to carbon injection. These immunomodulatory effects could be determined with extraction conditions of crude ${\beta}$-glucan.

Keywords

oat bran;crude ${\beta}$-glucan;murine peritoneal macrophages;nitric oxide;carbon clearance

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