Antimicrobial Effects of Camellia Japonica L. Leaves Extract on Food-borne Pathogenic Microorganisms

동백나무(Camellia japonica L.) 잎 추출물이 식품유해 미생물에 미치는 항균 효과

  • Hahn, Young-Sook (Department of Food and Nutrition, Sungshin Women's University)
  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2005.02.28

Abstract

Antimicrobial effects of Camellia japonica L. were determined against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes using paper disc method, and minimum inhibitory concentrations (MICs) were measured. Methanol extract (MEex), water fraction (WAfr), and butanol fraction(BUfr) showed antimicrobial effects against all tested microorganisms, with MEex showing strong antimicrobial effect against S. aureus and L. monocytogenes, and WAfr, Bufr, and ethylacetate fraction (EAfr)against S. aureus. No effects were observed in n-hexane fraction (HEfr) and chloroform fraction (CHfr) against all tested microorganisms. All species grown in the medium adding fractions of Camellia Japonica L. leaves extact were inhibited from WAfr and BUfr, repectively.(meaning not clear) MEex showed over 25% inhibitory effect against all tested microorganisms. BUfr showed over 50% inhibitory effect against all microorganisms except L. monocytogenes. EAfr and WAfr showed over 30% effect against S. aureus and L. monocytogenes. MICs of MEex against S. typhimurium and BUfr against S. aureus were 625 g/mL, indicating C. japonica L. extract can exert antimicrobial activity even at low concentration.

Keywords

Camellia japonica L.;antimicrobial;methanol extract

References

  1. Kang DH, Fung DYC. Effect of diacetyl controlling Escherichia coli O157:H7 and Salmonella typhimurium in the presence of starter culture in a laboratory medium and during meat fermentation. J. Food Prot. 62: 975-979 (1999)
  2. Jean YO, Kim SI, Han YS, Kim KH. Screening of antimicrobial activity of the Plantain (Plantago asiatica L.) extract. Korean J. Soc. Food Sci. 14: 498-502 (1998)
  3. Sea KL, Kim DY, Yang SI. Studies on the antimicrobial effect of wasabi extracts. Korean J. Nutr. 28: 1073-1077 (1995)
  4. Kim KY, Davidson PM, Chung HJ, Antibacterial activity in extracts of Camellia japonica L. petals and its application to a model food system, J. Food Prot. 64: 1255-1260 (2001)
  5. Kong YJ, Park BK, Oh DH. Antimicrobial activity of quercus mongolica leaf ethanol extract and organic acids against foodborne microorganisms. Korean J. Food Sci. Technol. 33: 178-183 (2001)
  6. Oh DH, Ham SS, Park BK, Ahn C, Yu JY. Antimicrobial Activities of natural medicinal herbs on the food spoilage or foodborne disease microorganisms. Korean J. Food Sci. Technol. 30: 957-963 (1998)
  7. Park HO, Kim CM, Woo GJ, Park SH, Lee DH, Chang EJ, Park KH. Monitoring and trends analysis of food poisoning outbreaks occurred in recent years in Korea. J. Food Hyg. Safety, 16: 280-294 (2001)
  8. Yousef AE, EL-Shenawy MA, Marth EH. Inactivation and injury of Listeria monocytogenes in a minimal medium as affected by benzoic acid and incubation temperature. J. Food Sci. 54: 650-652 (1989) https://doi.org/10.1111/j.1365-2621.1989.tb04673.x
  9. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS. Screening of domestic plants with antibacterial activity. J. Korean Soc. Appl. Bio. Chem. 38: 584-589 (1995)
  10. Kang SK, Sung NK, Kim YD, Shin SC, Sea JS, Choi KS, Park SK. Screening of antimicrobial activity of leaf mustard (Bras sica Juncea) extract. J. Korean Soc. Food Nutr. 23: 1008-1013 (1994)
  11. Lee SH, Kim SK. Natural distribution and characteristics of populations of Camellia japonica in Korea. J. Korean Soc. Hort. Sci. 33: 196-208 (1992)
  12. Larocco KA, Martin SE. Effects of potassium sorbate alone and in combination with sodium chloride on the growth of Salmonella typhimurium 7136. J. Food Sci. 46: 568-570 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb04912.x
  13. Tabak M, Armom R, Potasman I, Neeman I. In vitro inhibition of Helicobacter pylori by extracts of thyme. J. Appl. Bacteriol. 80: 667-672 (1996) https://doi.org/10.1111/j.1365-2672.1996.tb03272.x
  14. Venkitanarayanan KS, Ezeike GOI, Hung YC, Doyle MP. Inactivation of Escherichia coli O157:H7 and and Listeria monocytogenes on plastic kitchen cutting boards by electrolyzed oxidizing water. J. Food Prot. 62: 857-860 (1999)
  15. Schobitz R, Zaror T, Leon O, Costa M. A bacteriocin from Carnobacterium piscicola for the control of Listeria monocytogenes in vacuum packaged meat. Food Microbiol. 16: 249-255 (1998) https://doi.org/10.1006/fmic.1998.0241
  16. Smith JL, Marmer BS. Growth temperature and action of lysozyme on Listeria monocytogenes. J. Food Sci. 56: 1101-1103 (1991) https://doi.org/10.1111/j.1365-2621.1991.tb14651.x
  17. Lee JJ, Kim SH, Chang BS, Lee JB, Huh CS, Kim TJ, Baek YJ. The antimicrobial activity of medicinal plants extracts against Helicobacter Pylori. Korean J. Food Sci. Technol. 31: 764-770 (1999)
  18. Park UK, Chang DS, Cho HR. Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr. 21: 91-96 (1992)
  19. Taormina PJ, Beuchat LR. Behavior of enterohemorrhagic Escherichia coli O157:H7 on alfalfa sprouts during the sprouting process as influenced by treatments with various chemicals. J. Food Prot. 62: 850-856 (1999)
  20. Juven BJ, Barefoot SF, Pierson MD, Maccaskill LH, Smith B. Growth and survival of Listeria monocytogenes in vacuum-packaged ground beef inoculated with Lactobacillus alimentarius Flora Cam L-2. J. Food Prot. 61: 551-556 (1998)
  21. Amsterdam D. Susceptibility Testing of Antimicrobials in Liquid Media, Antibiotics in Laboratory Medicine. 4th ed. Williams and Wilkins, MD, USA. pp. 52-111 (1996)
  22. Fujita Y, Fujita H, Yoshikawa H. Comparative biochemical and chemotaxonomical studies of the plants of Theaceae (I), Essential oils of Camellia sasanqua Thunb., C. japonica Linn., and Thea sinensis Linn. Osaka kogyo Gijutsu Shikensho Kigo. 25: 198-202 (1973)
  23. Conner DE, Beuchat LR. Effect of essential oils from plants on growth of food spoilage yeast. J. Food Sci. 49: 429-434 (1984) https://doi.org/10.1111/j.1365-2621.1984.tb12437.x
  24. Yoshikawa M, Harada E, Murakami T, Matsuda H, Yamahara J, Murakami N. Camellia saponnins B1, B2, C1 and C2, new type inhibitors of ethanol absorption in rats from the seeds of Camellia japonica L, Chem. Pharm. Bull. 42: 742-749 (1994) https://doi.org/10.1248/cpb.42.742
  25. Sapers GM, Miler RL, Mattazzo AM. Effectiveness of sanitizing agents in inactivating Escherichia coli in golden delicious apples. J. Food Sci. 62: 734-737 (1999)
  26. Shin DH, Kim MS, Han JS. Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates against food-born bacteria. Korean J. Food Sci. Technol. 29: 808-816 (1997)
  27. Nam SH, Yang MS. Antibacterial activities of extracts from chrysanthemun boreale M. J. Korean Soc. Appl. Bio. Chem. 38: 269-272 (1995)
  28. Sheo HJ. The Antibacterial action of garlic, onion, ginger and red pepper juice. J. Korean Soc. Food Sci. Nutr. 28: 94-99 (1999)
  29. Lee SH, Lim YS. Antimicrobial effects of Schizandra chinensis extract on pathogenic microorganism. J. Korean Soc. Food Sci. Nutr. 27: 239-243 (1998)
  30. Lee CB. Korean plants pictorial book. Hyangmoon Publishing Co., Korea (1999)
  31. Ahn YS, Shin DH, Baek NI. Isolation and identification of active antimicrobial substance against Listeria monocytogenes from Ruta graveolens Linne. Korean J. Food Sci. Technol. 32: 1379-1388 (2000)
  32. Beuchat LR. Survival of enterohemorrhagic Escherichia coli O157:H7 in bovine feces applied to lettuce and effectiveness of chlorinated water as disinfectant. J. Food Prot. 62: 845-849 (1999)
  33. Castillo A, Lucia LM, Kemp GK, Acuff GR. Reduction of Escherichia coli O157:H7 and Salmonella typhimurium on beef carcass surfaces using acidified sodium chloride. J. Food Prot. 62: 580-584 (1999)
  34. Stecchini ML, Luch RD, Bortolussi G, Deltorre M. Evaluation of lactic acid and monolaurin to control Listeria monocytogenes on stacchino cheese. Food Microbiol. 13: 483-488 (1996) https://doi.org/10.1006/fmic.1996.0055
  35. Itokawa H, Nakajima H, Ikuta A, Iitaka Y. Two teiterpenes from the flowers of Camellia japonica. Phytochem. 20: 2539-2542 (1981) https://doi.org/10.1016/0031-9422(81)83089-0