Extraction Characteristics of Flavonoids from Lonicera flos by Supercritical Fluid Carbon Dioxide ($SF-CO_2$) with Co-solvent

초임계유체 $CO_2$ 및 Co-solvent 첨가에 따른 금은화(Lonicera fles)의 Flavonoid류 추출특성

  • Suh, Sang-Chul (Department of Food Science and Technology, Kyungpook National University) ;
  • Cho, Sung-Gill (Department of Food Science and Technology, Kyungpook National University) ;
  • Hong, Joo-Heon (Department of Food Science and Technology, Kyungpook National University) ;
  • Choi, Yong-Hee (Department of Food Science and Technology, Kyungpook National University)
  • 서상철 (경북대학교 식품공학과) ;
  • 조성길 (경북대학교 식품공학과) ;
  • 홍주헌 (경북대학교 식품공학과) ;
  • 최용희 (경북대학교 식품공학과)
  • Published : 2005.04.30

Abstract

Effects of co-solvent polarity, citric acid, pressure, temperature, run time, and co-solvent ratio on extraction of major flavonoids from Lonicera Flos were investigated using supercritical fluid $CO_{2}(SF-CO_{2})$. HPLC analysis revealed addition of pure methanol resulted in low extraction yield of major flavonoids, luteoloin (Lu), Quercetin (Qu), Apigenin (Ap). Under same condition, as co-solvent polarity increased, yields of major flavonoids increased gradually, At optimum co-solvent extraction condirion of 60% aqueous methanol (10%, v/v), yields of Lu, Qu, and Ap were 42.09, 28.18, and 3.49 mg/100 g, respectively. Addition of citric acid to 60% aqueous methanol gave higher, with addition of 1% citrie acid resulting in highest yields of 63.2 (Lu), 39.35 (Qu), and 5.79 (Ap) mg/100 g. Optimum extraction conditions of major flavonoids were 200 bar, $50^{\circ}C$, 60 min, and $CO_{2}$-methanol-water(20: 1.8: 1.2).

Keywords

lonicera flos;flavonoids;supercritical fluid extraction;co-solvent

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