Effects of Enzyme Treatment on Physicochemical Characteristics of Small Red Bean Percolate

팥 침출액의 이화학적 특성에 대한 효소처리의 영향

  • Hwang, Cheol-Seung (Lotte R&D Center) ;
  • Jeong, Do-Yeong (Faculty of Biotechnology (Food Science & Technology Major), Chonbuk National University) ;
  • Kim, Yong-Suk (Research Center for Industrial Development of BioFood Materials, Chonbuk Notional University) ;
  • Na, Jong-Min (Daedoo Food Co., Ltd.) ;
  • Shin, Dong-Hwa (Faculty of Biotechnology (Food Science & Technology Major), Chonbuk National University)
  • 황철승 (롯데중앙연구소) ;
  • 정도영 (전북대학교 응용생물공학부(식품공학 전공)) ;
  • 김용석 (전북대학교 바이오식품 소재개발 및 산업화 연구센터) ;
  • 나종민 ((주)대두식품) ;
  • 신동화 (전북대학교 응용생물공학부(식품공학 전공))
  • Published : 2005.04.30

Abstract

Effect of enzyme treatment on physicochemical characteristics of small tea bean percolate were estimated. Three types of small red bean percolate were prepared by heat treatment for 30 min at $95^{\circ}C$ (1st), 30 min at $95^{\circ}C$ (2nd), and 40 min at $120^{\circ}C$ (3rd). They were then treated with 0.5% ${\alpha}-amylase$ (v/v) for 4 hr at $108^{\circ}C$ (${\alpha}-amylase$ treatment), then with 0.5% ${\beta}-amylase$ (v/v) for 4 hr at $60^{\circ}C$ (${\alpha}-$ and ${\beta}-amylase$ treatment). Crude saponin contents of 1st-3rd percolates were 0.82, 1.44, and 1.52 mg/g, respectively. ${\circ}Brix$ of small red bean percolates treated with enzymes increased to $0.8-1.2\;{\circ}Brix$ with 2nd and 3rd percolates showing no significant difference between ${\alpha}-amylase$ and ${\alpha}-$ and ${\beta}-amylase$ treatments. pH of 3rd percolate treated with ${\alpha}-$ and ${\beta}-amylase$ decreased from initial 6.2 to 4.7. Hunter L value of small red bean percolate treated with ${\alpha}-$ and ${\beta}-$ decreased, whereas a and b values increased. Small red bean beverage made with 3rd percolate showed high score in flavor, taste, and overall acceptability. Results suggest small red bean percolate treated with enzymes could be used for preparation of small red bean beverage.

Keywords

small red bean;small red bean percolate;small red bean beverage;${\alpha}-amylase$;${\beta}-amylase$

References

  1. Korea Rural Economic Institute. Food Balance Sheet 8. Dongamunhwa Printing Forum, Seoul, Korea. pp. 136-137, pp. 162-163 (2002)
  2. National Rural Living Science Institute. Food Composition Table. Sixth Revision II. Sangroksa, Seoul. Korea, p. 29 (2001)
  3. Chang HG Information on Food for the Health of Modem People. Shinkwang Publishing Co., Seoul, Korea. pp. 46-50 (1999)
  4. Choi SY, Jeong YJ, Lee SJ, Chi OH, Chegal SA. Food and Health for Modem People. Dongmyung-Sa, Seoul, Korea. pp. 244-246 (2002)
  5. AOAC. Official Method of Analysis. 16th ed. The Association of Official Analytical Chemists, Washington, DC, USA (1995)
  6. SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1998)
  7. Korea Food and Drug Administration. Food Code. Moonyoungsa, Seoul, Korea. pp. 407-408 (2002)
  8. Koh KJ, Shin DB, Lee YC. Physicochemical properties of aqueous extracts in small red bean, mung bean and black soybean. Korean J. Food Sci. TechnoI. 29: 854-859 (1997)
  9. Shiraiwa M, Harada K, Okubo K. Composition and content of saponins in soybean seed according to variety, cultivation year and maturity. Agric. BioI. Chem. 55: 323-331 (1991) https://doi.org/10.1271/bbb1961.55.323
  10. Koide T, Hashimoto Y, Kamei H, Kojima T, Hasegawa M, Terabe K. Antitumor effect of anthocyanin fractions extracted from red soybeans and red beans in vitro and in vivo. Cancer Biother. Radiopharm. 12: 277-280 (1997)
  11. Ministry of Agriculture and Forestry. Production of crops. Available from: http://www.naqs.go.kr/htmI/05_03_01.htm. Accessed July 12, 2003
  12. Fujiwara A, Kajita T. Analysis of azuki peel pigments and effects of water quality on color tone of 'Azuki ann'. Kateigaku Kenkyu 34: 1-7 (1987)
  13. National Rural Living Science Institute. Food Composition Table. Sixth Revision I. Sangroksa, Seoul, Korea, pp. 74-75 (2001)
  14. Kitagawa I, Yoshikawa M, Hayashi T, Taniyama T. Quantitative determination of soyasaponins of various origins and soybean products by means of HPLC. Yakugaku Zasshi 104: 275-279 (1984) https://doi.org/10.1248/yakushi1947.104.3_275
  15. Sohn KH, Yoon GS, Chun HJ, Chae SH. Comparison of physicochemical properties of various bean starches. Korean J. Soc. Food Sci. 6: 13-19 (1990)
  16. Ariga T, Hamano M. Radical scavenging action and its mode in procyanidins B-1 and B-3 from azuki beans to peroxyl radicals. Agric. BioI. Chem. 54: 2499-2504 (1990) https://doi.org/10.1271/bbb1961.54.2499
  17. Kim CH, Park JS, Sohn HS, Chung CWO Determination of isoflavone, total saponin, dietary fiber, soy oligosaccharides and lecithins from commercial soy products based on the one serving size - some bioactive compounds from commercialized soy products-. Korean J. Food Sci. TechnoI. 34: 96-102 (2002)
  18. Noh MJ, Kwon JH, Byun MW. Water-soluble components of small red bean and mung bean exposed to gamma irradiation and methyl bromide fumigation. Korean J. Food Sci. TechnoI. 33: 184-189 (2001)
  19. Cho JS. Food Materials. Munundang, Seoul, Korea, pp. 92-93 (1984)
  20. Ireland PA, Dziedzie SZ, Kearsley MW. Saponin content of soya and some commercial soya products by means of high performance liquid chromatography of the sapogenins. J. Sci. Food Agric. 37: 694-698 (1986) https://doi.org/10.1002/jsfa.2740370715
  21. Yoshida K, Sato Y, Okuno R, Kameda K, Isobe M, Kondo T. Structural analysis and measurement of anthocyanins from colored seed coats of Vigna, Phaseolus, and Glycine legumes. Biosci. Biotech. Biochem. 60: 589-593 (1996) https://doi.org/10.1271/bbb.60.589
  22. Ariga T, Koshiyama I, Fukushima D. Antioxidative properties of procyanidins B-1 and B-3 from azuki beans in aqueous systems. Agric. BioI. Chem. 52: 2717-2722 (1988) https://doi.org/10.1271/bbb1961.52.2717