Antimicrobial, Antihypertensive and Anticancer Activities of Garlic Extracts

마늘 추출물의 항균, 항고혈압 및 항암활성

  • Published : 2005.04.30


Antibacterial, antifungal, anticancer, and ACE inhibitory activities of methanol, ethyl acetate, chloroform, and hexane extracts of garlic were investigated. Methanol extract showed highest yield of 7.9-8.8 brix. Ethyl acetate and chloroform extracts showed strong antibacterial activities as determined by inhibition zone (8-16mm) against B. subtilis and P. aeruginosa, and good antifungal activities as determined by inhibition zone (8-30mm) against A. niger, M. miehei, and T. reesei. Wando, Banyans, Chinese, and Seosan garlic methanol extracts showed 65.6, 60.4, 70.1, and 55.3% ACE inhibitory activities, 42.3, 53.8, 50.4, and 54.5% anticancer activities against SNU-1, and 11.3, 20.2, 36.6, and 30.5% anticancer activities against HeLa, respectively.


garlic;antibacterial;antifungal;angiotensin converting enzyme (ACE) inhibitory activity;anticancer activity


  1. Stoll A, Seebeck E. Chemical investigation on alliin, the specific principle of garlic. Adv. Enzymol. 11: 377-399 (1951)
  2. Sharma KK. Sharma AL, Dwividi KK. Sharma PK. Effect of raw and boiled garlic on blood cholesterol in butter fat lipoemia. Ind. J. Nutr. Dictet. 13: 7-11 (1976)
  3. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-601 (1987)
  4. Ji WD. Jeong MS, Chung HC, Lee SJ, Chung YG. Antimicrobial activity and distilled components of garlic and ginger. Agric. Chem. Biotechnol. 40: 514-518 (1997)
  5. Kunio S. Isolation and characterization of angiotensin l-converting enzyme inhibitor dipeptides derived from Allium sativum L. (garlic). J. Nutr. Biochem. 9: 415-419 (1998)
  6. Sasaki J. Kita T, Ishita K, Uchisawa H. Matsue H. Antibacterial activity of garlic powder against Escherichia coli O-157. J. Nutr. Sci. Vitaminol. 45: 785-790 (1999)
  7. Harenbarg J, Giese C, Zimmermann R. Effects of dried garlic on blood coagulation, fibrinolysis, platelet aggregation and serum cholesterol levels in patients with hyperlipoproteinmia. Atherosclerosis 74: 247-249 (1988)
  8. Kumar M, Berwal JS. Sensitivity of food pathogens to garlic. J. Appl. Microbiol. 84: 213-215 (1998)
  9. Shea HJ. The antibacterial action of garlic, onion, ginger and red pepper juice. J. Korean Soc. Food Sci. Nutr. 28: 94-99 (1999)
  10. Park JG, Kwon NS, Kim JP, Oh SK. Establishment of SNUH cell lines in serum-free defined medium and SNU cell lines serum supplemented medium. J. Korean Cancer Assoc. 20: 105-116 (1998)
  11. Nakata T. Effect of fresh garlic extract on tumor growth. Jpn. J. Hyg. 27: 538-546 (1973)
  12. Rietz B, Isensee H, Strobach H, Makdessi S, Jacob R. Csrdioprotective actions of wild garlic (Allium ursinum) in ischemia and reperfusion. Mol. Cell. Biochem. 119: 143-150 (1993)
  13. Ham SS, Cui CB, Choi HT, Lee DS. Antimutagenic and cytotoxic effect do Allium victorialis extracts. Korean J. Food Preser. 11: 221-226 (2004)
  14. Akiko S, Michiniri T, Miyako I. Antibacterial effect of garlic extract on Vibrio parahaemolyticus in fish meat. J. Food Hyg. Soc. Japan 34: 63-67 (1993)
  15. Choi HK. A study on the antibacterial activity of garlic against Escherichia coli O-157. J. Korean Practical Arts Edu. 14: 159-167 (2001)
  16. Cushman DW, Cheung HS. Spectrophotometric asaay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971)
  17. Shin HK, Kim KS. Effect of garlic on the changes in blood pressure of hypertensive rats. J. Hanyang Med. CoIl. 9: 75-87 (1989)
  18. Dipaolo JA. Carruthers C. The effect of allicin from garlic on tumor growth. Cancer Res. 20: 431-439 (1960)
  19. Jin YH, Kwon OC, Sung NJ, Shin JH, Kang MJ. Effect of garlic on quality of low salted anchovy. Changes of general composition, titrable acidity and sensory evaluation. Culinary Soc. Korean Academy. 7: 49-70 (2001)
  20. Yamata Y, Azuma K. Evaluation of the in vitro antifungal activity of allicin. Anents Chemother. 11: 743-747 (1977)
  21. Ali M, Thomson M, Afzal M. Gaelic and onions: their effect on eicosanoid metabolism and its clinical relevance. Prostaglandins. Leukotrienes and Essential Fatty Acids. 62: 55-73 (2000)
  22. Cavallito CJ, Bailey JH, Buck JS. The antibacterial principle of Allium sativum III. Its procusor and essential oil of garlic. J. Am. Chem. Soc. 67: 1032-1040 (1976)
  23. Kim YS. Park KS, Kyung KH, Shim ST, Kim HK. Antibacterial activity of garlic extract against Escherichia coli. Korean J. Food Sci. Technol. 28: 730-735 (1996)
  24. Weisberger AS, Pensky J. Tumor inhibition by a sulfhydryl-blocking agent related to an active principle of garlic. Cancer Res. 18: 1301-1307 (1958)
  25. AOAC. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Washington, DC, USA (1990)