Fibrinolytic and Immunostimulating Activities of Bacillus spp. Strains Isolated from Chungkuk-jang

청국장에서 분리한 Bacillus spp. 균주의 혈전용해능 및 면역증강활성

  • Chang, Jin-Hee (Traditional Food Research Division, Korea Food Research Institute) ;
  • Shim, Youn-Young (Graduate School of Biotech., Korea University) ;
  • Kim, Seung-Ho (Traditional Food Research Division, Korea Food Research Institute) ;
  • Chee, Kyoo-Man (Graduate School of Biotech., Korea University) ;
  • Cha, Seong-Kwan (Traditional Food Research Division, Korea Food Research Institute)
  • Published : 2005.04.30


To make Chungkuk-jang with enhanced fibrinolytic and immunostimulating activities, 220 strains isolated from Chungkuk-jangs were tested, and 13 Bacillus strains with excellent proteolytic and polysaccharide-producing activities were selected and tested for their fibrinolytic and immunostimulating activities using fibrin plate method and RAW 264.7 cell line, respectively. To assess macrophage activation, contents of cytokines such as tumor necrosis factor ($TNF-{\alpha}$) and $interleukin-1{\alpha}$ and nitric oxide were measured. Three strains showing highest fibrinolytic and immunostimulating activities were identified as Bacillus licheniformis (CHKJ 1249, 1326) and Bacillus subtilis (CHKJ 1339).


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