Production of Reuterin by Immobilized Lactobacillus reuteri

Lactobacillus reuteri의 고정화 세포를 이용한 루테린 생산

  • Published : 2005.04.30


Lactobacillus reuteri residing in human and animal intestines converts glycerol into reuterin (antimicrobial substance) in anaerobic condition. Attempt was made to increase production efficiency of L. reuteri by employing immobilized cells. L. reuteri was immobilized in agarose beads, which were then reacted with 250 mM glycerol solution. Batch-type production of reuterin with immobilized cells (0.5% agarose beads) lasted for about 36 h, although reuterin production decreased with passage of time. In continuous-type production, period of reuterin production with immobilized cells was extended about twofold and production ratio increased 1.5-fold (502 mM) compared with suspended cells (315 mM). Maximum concentration of reuterin reached 47 mM at 80 min after reaction with glycerol solution. Results of this study indicate that immobilization of Lactobacillus reuteri in agarose beads increased reuterin production.


Lactobacillus reuteri;reuterin;immobilized cell;batch-type;continuous-type


  1. Chen CC, Chen JY, Lee SR. Growth inhibition of glycerol metabolites of Lactobacillus reuteri on microorganisms and human cancer cell lines. J. Chin. Agric. Chem. Soc. 37: 117-125 (1999)
  2. Luthi-Peng Q, Scharer S, Puhan Z. Production and stability of 3hydroxypropionaldehyde in Lactobacillus reuteri. Appl. MicrobioI. Biotechnol. 60: 73-80 (2002)
  3. Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie 70: 337-349 (1988)
  4. Dobrogosz WJ, Lindgren SE. Method of determining the presence of an antibiotic produced by Lactobacillus reuteri. U.S. patent 5,352,586 (1994)
  5. Talarico TL, Dobrogosz WJ. Chemical characterization of an antimicrobial substance produced by Lactobacillus reuteri. Antimicrob. Agents Chemother. 33: 674-679 (1989)
  6. Lindgren SE, Dobrogosz WJ. Antagonistic activities of lactic acid bacteria in food and feed fermentations. FEMS Microbiol. Rev. 87: 149-163 (1990)
  7. EI-Ziney MG, Debevere JM. The effect of reuterin on Listeria monocytogenes and Escherichia coli 0157:H7 in milk and cottage cheese. J. Food Prot. 61: 1275-1280 (1998)
  8. Daeschel MA. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technol. 4: 164-167 (1989)
  9. Speck ML, Dobrogosz WJ, Casas I. Lactobacillus reuteri in food supplementation. Food Technol. 7: 90-94 (1993)
  10. Yum EM, Kim JY, Shin HK, Ji GE. Production of reuterin by batch and continuous reactor and antimicrobial characteristics of reuterin. Korean J. Food Sci. Technol. 36: 111-115 (2004)
  11. Ahn C, Kim CH, Shin HK, Lee YM, Lee YS, Ji GE. Antibiosis of pediocin-producing Pediococcus sp. KCA1303-10 against Listeria monocytogenes in mixed cultures. J. Microbiol. Biotechnol. 13: 429-436 (2003)
  12. Ganzle MG, Holtzel A, Walter J, Jung G, Hammes WP. Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584. Appl. Environ. Microbiol. 66: 4325-4333 (2000)
  13. Cleveland J, Montville TJ, Nes IF, Chikindas ML. Bacteriocins: Safe, natural antimicrobials for food preservation. Int. J. Food Microbiol. 71: 1-20 (2001)