Fermentation Characteristics of Low Salted Kochujang Prepared with Mixture of Sub-materials

부원료를 혼합 첨가한 저식염 고추장의 발효 특성

  • Published : 2005.06.30


To reduce saft content of kochujang, various combinations of sub-materials such as ethanol mustard and chitosan were added to kochujang, and their effects on microbial characteristics, enzyme activities, and physicochemical characteristics of kochujang were investigated after 12 weeks of fermentation. Activities of ${\beta}$-amylase and pretense were low in ethanol-mustard-chitosan-added kochujang, whereas no significant difference was observed in ${\alpha$-amylase activity among all groups. Number of viable yeast cells decreased remarkably in mustard-added kochujang during late aging period, and anaerobic bacterial counts decreased in sub-material-added groups. Consistency of kochujang increased by addition of sub-materials, and oxidation-reduction potential was low in chitosan-added group. Mustard-chitosan-added kochujang showed lowest increase in total color difference(${\Dalta}E$) and decrease in water activity. PH of kochujang wns highest in mustard-chitosan-added kochujang, resulting in significantly increased titratable acidity. Addition of sub-material increased reducing sugar contents of kochujang, whereas ethanol production was significantly repressed in mustard-chitosan-added kochujang. Amino nitrogen content was Highest in mustard-chitosan-added kochujang during late aging period, whereas ammonia nitrogen content was lower in ethanol-mustard-added kochujang. Results of sensory evaluation indicated ethanol-mustard-added kochujang was more acceptable than other groups in taste and overall acceptability.


low salted kochujang;sub-materials;physicochemical properties


  1. Cho HO, Kim JG, Lee HJ, Kang JH, Lee TS. Brewing method and composition of traditional kochujang (red pepper paste) in junrabook-do area. J. Korean Agric. Chem. Soc. 24: 21-28 (1981)
  2. Oh HI, Shon SH, Kim JM. Physicochemical properties of kochujang prepared with Aspergillus oryzae, Bacillus licheniformis and Saccharomyces rouxii during fermentation. J. Korean Soc. Food Sci. Nutr. 29: 357-363 (2000)
  3. Kwan DJ, Jung JW, Kim JH, Park JY, Yoo JY, Koo YJ, Chung KS. Studies on establishment of optimal aging time of Korean traditional kochujang. Agric. Chem. Biotech. 39: 127-133 (1996)
  4. Kim YS, Cha J, Jung SW, Park EJ, Kim JO. Changes of physico-chemical characteristics and development of new quality indices for industry-produced koji kochujang. Korean J. Food Sci. Technol. 26: 453-458 (1994)
  5. Yun YS, Kim KS, Lee YN. Antibacterial and antifungal effect of chitosan. J. Chitin Chitosan 4: 8-14 (1999)
  6. SAS Institute, Inc. SAS User's Guide. Statistical Analysis System Institute, Cary, NC, USA (1992)
  7. Lee JM, Jang JH, Oh NS, Han MS. Bacterial distribution of kochujang. Korean J. Food Sci. Technol. 28: 260-266 (1996)
  8. Lee KS, Kim DH. Trial manufacture of low-salted kochujang (red pepper soybean paste) by the addition of alcohol. Korean J. Food Sci. Technol. 17: 146-154(1985)
  9. Lee TS. Studies on the brewing of kochujang (red papper paste) by the addition of yeasts. J. Korean Agri. Chem. Soc. 22: 65-90 (1979)
  10. Choi JY, Lee TS, Noh BS. Quality characteristics of the kochujang prepared with mixture of meju and koji during fermentation. Korean J. Food Sci. Technol. 32: 125-131 (2000)
  11. Anson ML. Estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22: 79-89 (1938) https://doi.org/10.1085/jgp.22.1.79
  12. Jung SW, Kim YH, Koo MS, Shin DB. Changes in physicochemical properties of industry-type kochujang during storage. Korean J. Food Sci. Technol. 26: 403-410 (1994)
  13. Lee KS, Kim DH. Effect of sake cake on the quality of low salted kochujang. Korean J. Food Sci. Technol. 23: 109-115 (1991)
  14. Shin DH, Ahn EY, Kim YS, Oh JA. Fermentation characteristics of kochujang containing horseradish or mustard. Korean J. Food Sci. Technol. 32: 1350-1357 (2000)
  15. Moon TW, Kim ZU. Some chemical physical characteristics and acceptability of kochujang from various starch sources. J. Korean Agric. Chem. Soc. 31: 387-393 (1988)
  16. Kim YS. Studies on the changes in physicochemical characteristics and volatile flavor compounds of traditional kochujang during fermentation. PhD thesis, University of King Sejong, Seoul, Korea (1993)
  17. Oh JY, Kim YS, Shin DH. Changes in physicochemical characteristics of low-salted kochujang with natural preservatives during fermentation. Korean J. Food Sci. Technol. 34: 835-841 (2002)
  18. Official methods of miso analysis. Institute of Miso Technologists, Tokyo, Japan, pp. 1-34 (1968)
  19. Kim DH, Lee JS. Effect of condiments on the physicochemical characteristics of traditional kochujang during fermentation. Korean J. Food Sci. Technol. 33: 353-360 (2001)
  20. Chun MS, Lee TS, Noh BS. Effect of gamma-irradiation on quality of kochujang during storage. Foods Biotechnol. 1: 117-122 (1992)
  21. Jung YC, Choi WJ, Oh NS, Han MS. Distribution and physiological characteristics of yeasts in traditional and commercial kochujang. Korean J. Food Sci. Technol. 28: 253-259 (1996)
  22. Cho HO, Park SA, Kim JG Effect of traditional and improved kochujang koji on the quality improvement of traditional kochujang. Korean J. Food Sci. Technol. 13: 319-327 (1981)
  23. Kim DH, Yang SE. Fermentation characteristics of low salted kochujang prepared with sub-materials. Korean J. Food Sci. Technol. 36: 97-104 (2004)
  24. Yamamoto Y, Higashi K, Yoshii H. Inhibitory activity of ethanol on food spoilage bacteria. Nippon Shokuhin Kogyo Gakkaishi 31: 531-535(1984) https://doi.org/10.3136/nskkk1962.31.8_531
  25. Kim DH, Kwon YM. Effect of storage conditions on the microbiological and physicochemical characteristics of traditional kochujang. Korean. J. Food. Sci. Technol. 33: 589-595 (2001)
  26. Fuwa HA. A new method for microdetermination of amylase activity by the use of amylose as the substrate. J. Biochem. 41: 583-588(1954) https://doi.org/10.1093/oxfordjournals.jbchem.a126476
  27. Yeo YK, Kim ZU. Studies on the standardization of the processing conditional of Ko-Choo-Jang(red pepper paste). J. Korean Agric. Chem. Soc. 21: 16-21 (1978)
  28. Shin DH, Kim DH, Choi U, Lim MS, An EY. Physicochemical characteristics of traditional kochujang prepared with various raw materials. Korean J. Food Sci. Technol. 29: 907-912 (1997)
  29. Jay JM. Modern Food Microbiology, 6th ed. Aspen Publishers, Inc., Gaithersburg, MD, USA. pp. 45-47 (2000)