Antibacterial Activity of Terminalia chebula Retz. Extract Against Food Spoilage Microorganisms

식품부패세균에 대한 가자(Terminalia chebula Retz.) 추출물의 항균활성

  • Published : 2005.06.30


Antibacterial activities of water and 70% ethanol extracts of Terminalia chebula Retz were investigated. Fractions were prepared by step-wise fractionation of water and 70% ethanol extracts using acetone, hexane, chloroform and butanol. Butanol fraction showed best antibacterial activities. Water and 70% ethanol extracts of T. chebula Retz. had significantly high pyrogallol content among 13 phenolic compound analysed by HPLC, and pyrogallol (standard) showed highest activities against several food spoilage microorganism.


Terminalia chebula Retz;antibacterial effect;polyphenolic compound;HPLC


  1. Golovinsky EV, Maneva LS, Angelov I I, Veljanova KD, Sniker DJ, Stankevich EK. Antibacterial and antitumor activity of some derivatives of ureidosuccinic acid. Neoplasma 23:43-46(1976)
  2. Oguni I, Yamada M. Protection against cancer risk by green tea and antibacterial activity of tea catechin against Helicobacter pylori. September 3, Lotte Hotel, Seoul, Korea. 4th Intl. Symposium on green tea, Seoul, Korea. The Korean Society of Food Science and Technology, Seoul, Korea (1997)
  3. Lee KS, Kim SH, Sim KC, Park CS, Shin YS. Antimicrobial activity of Terminalia chebula retz. extract against intestinal pathogens. Korean J. Food Nutr. 10: 559-563 (1997)
  4. Branen AL, Go HC, Genske RP. Purification and properties of antimicrobial substances produced by Streptococcus diacetiliactis and Luconostoc citrovorum. J. Food Sci. 40: 446-453 (1975)
  5. Mori A, Nishino C, Enoki N, Tawata S. Antibacterial activity and mode of action of plant flavonoids against Plotgus rulgaris and Staphylococcus aures. Phytochem. 26: 2231-2240 (1987)
  6. Orrjala J, Erdelmeier CA, Wright AD, Rali T, Sticher O. Five new prenylated $\rho$-hydroxybenzonic acid derivatives with antimicrobial and molluscicidal activity from Piper aduncum leaves. Plants Med. 59: 546-551 (1993)
  7. Ayres HM, Payne DN, Furr JR, Russell AD. Use of Malthus-AT system to assess the efficacy of permeabilizing agents on the activity of antibacterial agents Pseudomonas aeruginosa. Lett. Appl. Microbiol. 26: 422-426 (1998)
  8. Tomas-Barberan FA, Msonthi JD, Hostettann K. Antifungal epi-cuticular methylated flavonoids from Helichrysum intens. Phytochem. 27: 753-760 (1988)
  9. Do JR, Kang SN, Kim KJ, Jo JH, Lee SW. Antimicrobial, antioxidant activity and phenolic compounds in the medical plant water extracts. Food Sci. Biotechnol. 13: 640-645 (2004)
  10. Nepka C, Sivridis E, Antonoglou O, Kortsaris A, Georgellis A, Taitzoglou I, Hytoroglou P, Papadimitriou C, Zintzaras I, Kouretas D. Chemopreventive activity of very low dose dietary tannic acid administration in hepatoma bearing C3H male mice. Cancer Letters 141: 57-62 (1999)
  11. Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol. Res. 152: 239-245 (1997)
  12. Ikigai H, Nakae T, Hara Y, Shimamura T. Bactericidal catechins damage the lipid bilayer. Biochim. Biophys. Acta 1147: 132-136 (1993)
  13. Kim MS, Lee DC, Hong JE, Chang KS, Cho HY, Kwon YK, Kim HY. Antimicrobial effects of ethanol extracts from Korean and Indonesian plants. Korean J. Food Sci. Technol. 32: 949-958 (2000)
  14. Ammar S, Michael H, Pirkko H, Kalevi P. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. J. Ethnopharmacol. 81: 327-336 (2002)
  15. Conner DE, Beuchat LR. Effect of essential oils from plants on growth of food spoilage yeast. J. Food Sci. 49: 429-437 (1984)
  16. Kim JS, Lee GD, Kwon JH, Yoon HS. Identification of phenolic antioxidative components in Terminalia Chebula Retz. J. Korean Agric. Chem. Soc. 36: 239-243 (1993)
  17. Lee SH, Lim YS. Antimicrobial effects of Schizandra chinensis extract on pathogenic microorganism. J. Korean Soc. Food Sci. Nutr. 27:239-243(1998)
  18. Ravn H, Brimer I. Structure and antibacterial activity of plan-tamajoside, a caffeic acid sugar ester from Plantage major subsp. major. Phytochem. 27: 3433-3441 (1988)
  19. Baratta MT, Dorman HJD, Deans SG, Figueiredo AC, Barroso JG, Ruberto G. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragr. J. 13: 235-244 (1998)<235::AID-FFJ733>3.0.CO;2-T