Characteristics of ${\beta}$-Glucosidase Secreted by Trichoderma reesei KCTC 6952

Trichoderma reesei KCTC 6952로부터 분비된 ${\beta}$-glucosidase의 특성

  • Published : 2005.06.30

Abstract

Trichoderma reesei KCTC 6952 possesses cellulase system consisting of three enzymatic components necessary to synergistically hydrolyze crystalline cellulose, among which ${\beta}$-glucosidase effectively releases glucose from glycoside derivatives. ${\beta}$-Glucosidase of T. reesei KCTC 6952 grown in modified Mandels' medium showed maximum activity(1.33 unit/mL) 4 days after initiation of growth. Optimal reaction conditions of the enzyme were 50 mM sodium acetate (pH 5) at $70^{\circ}C$ for 10 min. Enzymatic activities stabilized below $50^{\circ}C$ at pH range of 4-5. Results show ${\beta}$-glucosidase exerted its catalytic activities at relatively high temperatures and broad pH range.

Keywords

Trichoderma reesei;${\beta}$-glucosidase;activity;stability;spent culture medium

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