Components and Biological Activity of Aqueous Extract Isolated from Winged Stem of Euonymus alatus

화살나무 물 추출물의 구성성분과 생리활성

  • Oh, Bong-Yun (Glycomics Team, Korea Basic Science Institute) ;
  • Hwang, Soo-Kyung (Glycomics Team, Korea Basic Science Institute) ;
  • Cheong, Mi-Young (Glycomics Team, Korea Basic Science Institute) ;
  • Sin, Hong-Sig (Chebigen, Inc., Jeollabuk-do Center for Biotechnology & Bioventure) ;
  • Park, Bock-Hee (Department of Food and Nutrition, Mokpo National University) ;
  • Lee, Jeong-Ho (Department of Microbiology & Immunology Chonbuk National University Medical School) ;
  • Kim, Soo-Hyun (Glycomics Team, Korea Basic Science Institute)
  • 오봉윤 (한국기초과학지원연구원 글라이코믹스팀) ;
  • 황수경 (한국기초과학지원연구원 글라이코믹스팀) ;
  • 정미영 (한국기초과학지원연구원 글라이코믹스팀) ;
  • 신홍식 (케비젠(주)) ;
  • 박복희 (목포대학교 식품영양학과) ;
  • 이정호 (전북대학교 의과대학 미생물 및 면역학과) ;
  • 김수현 (한국기초과학지원연구원 글라이코믹스팀)
  • Published : 2005.12.31

Abstract

Although Euonymus alatus (EA) has been used as traditional medicine for cancer treatment, exact substances involved in curing of the disease are not yet known. Free radical scavenging and reactive oxygen species (ROS) removal activities of aqueous extract components isolated from winged stem of EA in animal cell line were investigated. Aqueous extract of EA (AEEA) was fractionated by ultrafiltration. All fractions mainly consisted of polysaccharide (44.8%), protein (2.1%), small amounts of phenol compounds and organic acids. Antioxidant activity of AEEA increased depending on concentration fractions, as determined by 1,1-diphenyl-2-picrylhydrazyl method. ROS removal activity was visualized in Chinese hamster ovary cell line using laser scanning confocal microscope, and AEEA activity increased in order of F IV>F III>F I>F II. These results suggest AETA has bioactive carbohydrates with potentials as functional foods and antioxidants.

Keywords

Euonymus alatus;bioactive carbohydrates;free radical scavenging activity;ROS removal activity

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