Comparison of Colorimetric Methods for the Determination of Flavonoid in Propolis Extract Products

프로폴리스추출물 제품의 플라보노이드 함량분석을 위한 비색법의 비교

  • Kim, Eun-Jeong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Hwa-Jung (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kim, Hye-Jeong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Nam, Hye-Seon (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Mi-Keong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kim, Hae-Young (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Jin-Ha (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kang, Yun-Sook (Nutrition of Functional Food Headquarters, Korea Food and Drug Administration) ;
  • Lee, Jong-Ok (Food Headquarters, Korea Food and Drug Administration) ;
  • Kim, Hee-Yun (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration)
  • 김은정 (경인지방식품의약품안전청 시험분석팀) ;
  • 이화정 (경인지방식품의약품안전청 시험분석팀) ;
  • 김혜정 (경인지방식품의약품안전청 시험분석팀) ;
  • 남혜선 (경인지방식품의약품안전청 시험분석팀) ;
  • 이미경 (경인지방식품의약품안전청 시험분석팀) ;
  • 김해영 (경인지방식품의약품안전청 시험분석팀) ;
  • 이진하 (경인지방식품의약품안전청 시험분석팀) ;
  • 강윤숙 (식품의약품안전청 영양기능식품본부) ;
  • 이종옥 (식품의약품안전청 식품본부) ;
  • 김희연 (경인지방식품의약품안전청 시험분석팀)
  • Published : 2005.12.31

Abstract

Quantitative analysis of flavonoids in commercial propolis extract products were compared by three colorimetric methods; aluminum chloride method, dinitrophenylhydrazine method and aluminum nitrate method, Aluminum nitrate method in Korea Health Supplement Food Code was proved to be specific only for flavones and flavonols same as aluminum chloride method, while dinitrophenylhydrazine method was specific for flavanones and dihydroflavonols. Therefore, the sum of flavonoid contents determined by 2,4-Dinitrophenylhydrazine method and aluminum nitrate method may represent the real content of total flavonoids. As for the 25 commercial propolis extract products examined, the contents of flavonoid varied from 2.15% to 9.53% except for one product.

Keywords

propolis;flavonoid;colorimetric method;aluminum nitrate;aluminum chloride;2,4-dinitrophenylhydrazine

References

  1. Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 35: 347-363 (1998)
  2. Maklham KR, Mitchell KA, Wilkins AL, Daldy JA, Lu Y. HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry 42: 205-211 (1996) https://doi.org/10.1016/0031-9422(96)83286-9
  3. Kujumgiev A, Svetkova IT, Yu Serkedjieva, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J. Ethnopharmacol. 64: 235-240 (1999) https://doi.org/10.1016/S0378-8741(98)00131-7
  4. Greenway W, Scaysbrool T, Whatley FR. The composition and plant origins of propolis: a report of work at oxford. Bee World 71: 107-118 (1990) https://doi.org/10.1080/0005772X.1990.11099047
  5. Nagy M, Grancai D. Colorimetric determination of flavanones in propolis. Pharmazie 51: 100-101 (1996)
  6. Chang CC, Yang MH, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10: 178-182 (2002)
  7. Korea Food and Drug Administration. Health Supplement Food Code. KFDA, Seoul, Korea (2004)
  8. Didry N, Dubreuil L, Pinkas M. Analysis of propolis: some parameters and procedures for chemical quality control. J. Apicult. Res. 37: 99-105 (1998) https://doi.org/10.1080/00218839.1998.11100961
  9. Mirzoeva OK, Calder PC. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostaglandins, Leukotrienes Essent. Fatty acids. 55: 441-449 (1996) https://doi.org/10.1016/S0952-3278(96)90129-5
  10. Vennat B, Arvouet-Grand A, Gross D, Pourrat A. Qualitative and quantitive analysis of flavonoids and identification of phenolic acids from a propolis extract. J. Pharm. Belg. 50: 438-444 (1995)
  11. Christov R, Bankova V. Gas chromatographic analysis of underivatized phenolic constituents from propolis using an electron-capture detector. J. Chromatogr. 623: 182-185 (1992) https://doi.org/10.1016/0021-9673(92)85316-L