Research for Selecting The Optimized Vitamin C Analysis Method

최적 비타민 C 분석법 선정에 관한 연구

  • 최원선 (매일유업(주) 중앙연구소) ;
  • 김영진 (매일유업(주) 중앙연구소) ;
  • 정진영 (매일유업(주) 중앙연구소) ;
  • 김태진 (매일유업(주) 중앙연구소) ;
  • 정병문 (매일유업(주) 중앙연구소) ;
  • 김응률 (매일유업(주) 중앙연구소) ;
  • 정후길 (매일유업(주) 중앙연구소) ;
  • 전호남 (매일유업(주) 중앙연구소)
  • Published : 2005.12.31


2,4-Dinitrophenylhydrazine (DHP), indophenol, and HPLC methods, which are generally used to analyze vitamin C, were evaluated by comparing changes in vitamin C contents in various samples kept at 10 and $20^{\circ}C$. Amount of total ascorbic acid (TAA) in yoghurt A kept at $10^{\circ}C$, as measured by DNP method, decreased from 26.7mg/100mL for early period to 22.8mg/100mL for final period of storage, whereas, on 3rd and 10th storage days, increased contrary to general results. Using indophenol, TAA measured at 28.6 and 9.5mg/100mL, and 30.5 and 14.6mg/100mL using HPLC far early and final periods, respectively. With indophenol and HPLC, TAA amount showed steady tendency to decrease. In conclusion, HPLC is the most suitable method to analyze vitamin C content, and indophenol method can be also used during early storage stage.


ascorbic acid;indophenol;2,4-dinitrophenylhydrazine;HPLC


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