The Study on the Methylmercury Analysis and the Monitoring of Total Mercury and Methylmercury in Fish

어류 중 메틸수은 분석법 확립 및 모니터링

  • 김희연 (경인지방식품의약품안전청 시험분석팀) ;
  • 정소영 (식품의약품안전청 식품평가부) ;
  • 소유섭 (식품의약품안전청 식품평가부) ;
  • 오금순 (식품의약품안전청 식품평가부) ;
  • 박성수 (식품의약품안전청 식품평가부) ;
  • 서정혁 (식품의약품안전청 식품평가부) ;
  • 이은주 (식품의약품안전청 식품평가부) ;
  • 이윤동 (식품의약품안전청 식품평가부) ;
  • 최우정 (식품의약품안전청 식품평가부) ;
  • 엄지윤 (식품의약품안전청 식품평가부) ;
  • 송민수 (식품의약품안전청 식품평가부) ;
  • 이종옥 (식품의약품안전청 식품평가부) ;
  • 우건조 (식품의약품안전청 식품평가부)
  • Published : 2005.12.31

Abstract

Procedure for analysis of methylmercury in fish was developed, involving addition of HCl, extraction with toluene, and clean-up using L-cystein solution. Obtained extract is analyzed by gas chromatography with electron capture detector using Ulbon HR-Thermon-Hg column. Detection limit and recovery of the method were 0.005mg/kg (expressed as Hg), 98-107 (103%), respectively. Total mercury and methylmercury concentrations in 175 commercial fish samples ranged from [mean-max (mean), unit: mg/kg]: 0.014-1.200 (0.270) and 0.006-0.901 (0.168) in tuna-fish, 0.020-0.934 (0.323) and 0.012-0.553 (0.149) in martin-fish, 0.082-0.782 (0.391) and 0.040-0.436(0.201) in shark, 0,023-0.031 (0.026) and 0,013-0.018 (0.015) in salmon, 0.098-0.193 (0.133) and 0.031-0.015(0.090) in tilefish, and 0,031-0.214 (0.089) and 0.016-0.093 (0.042) in canned tuna respectively. No sample of analyzed fish exceeded 1.0mg/kg wet wt., limit for methylmercury established by Codex. In all species examined, estimated weekly intake was lower than Provisional Tolerable Weekly Intake recommended by the JECFA (the Joint FAO/WHO Expert Committee on Food Additives).

Keywords

methylmercury;tuna-fish;marlin-fish;shark;canned tuna;gas chromatography

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