Effect of NADH-Dependent Enzymes Related to Oxygen Metabolism on Elimination of Oxygen-Stress of Bifidobacteria

NADH요구 산소대사관련 효소가 bifidobacteria의 산소스트레스 제거에 미치는 영향

  • Ahn, Jun-Bae (Department of Culinary Arts & Food Technology, Youngdong University) ;
  • Park, Jong-Hyun (Department of Food and Bioengineering, Kyungwon University)
  • 안준배 (영동대학교 호텔식품외식학부) ;
  • 박종현 (경원대학교 분자식품생명공학과)
  • Published : 2005.12.31

Abstract

Selection of oxygen-tolerant strains and elucidation of their oxygen tolerance mechanism were crucial for effective use of bifidobacteria. Oxygen-tolerant bifidobacteria were able to significantly remove environmental oxygen (oxygen removal activity) as compared to oxygen-sensitive strains. Most oxygen removal activity was inhibited by heat treatment and exposure to extreme pH (2.0) of bifidobacterial cell. NADH oxidase was major enzyme related to oxygen removal activity. Oxygen-tolerant bifidobacteria possessed high NADH peroxidase activity level to detoxify $H_2O_2$ formed from reaction of NADH oxidase. Addition of oxygen to anaerobic culture broth significantly increased activities of HADH oxidase and NADH peroxidase within 1hr and rapid increment of oxygen concentration was prevented. Results showed NADH oxidase and NADH peroxidase of oxygen-tolerant bifidobacteria played important roles in elimination of oxygen and oxygen metabolite $(H_2O_2)$.

Keywords

oxygen-tolerant bifidobacteria;NADH oxidase;NADH peroxidase;oxygen removal activity

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