녹차, 메밀 및 포도잎 추출물 첨가식이가 동맥경화유발식이를 급여한 F1B Golden Syrian 햄스터의 지질대사와 대동맥의 지방 축적에 미치는 영향

The Effects of Dietary Supplements Included Green Tea, Buckwheat and Grape Leaf Extract on Lipid Metabolism and on Regression of Fatty Streak Lesions in F1B Golden Syrian Hamsters Fed the Atherogenic Diet

  • 발행 : 2005.12.31

초록

7주령의 F1B golden Syrian 햄스터에게 동맥경화 유발식이에 녹차추출물, 메밀추출물 및 포도잎 추출물을 함유한 타블렛 제품을 각각 5%와 10%로 첨가하여 7주간 사육하였을 때 햄스터 체내의 지질성분과 대동맥 내에서의 지방의 축적 정도에 미치는 영향을 조사하였다. 실험 결과 녹차, 메밀, 포도잎 추출물을 함유한 타블렛 제품은 동맥경화유발식이를 섭취하는 햄스터의 혈중 중성지방과 총콜레스테롤치를 감소시켰고, 대동맥궁내에서의 지방의 축적을 예방하였고, 간장내 중성지방과 총콜레스테롤치를 감소시키는 것으로 나타났다. 특히 5%를 첨가한 군보다 10%를 첨가한 군에서 더 높은 효과를 나타내어 농도 의존적인 경향을 보였다. 이러한 결과를 통해 녹차, 메밀 및 포도잎 추출물 분말을 이용하여 식이 보조제의 형태로 개발한 타블렛 제품이 동맥경화를 예방하는 식품으로써 효과가 있음을 확인할 수 있었다.

키워드

green tea;buckwheat;grape leaf;foam cell accumulation;atherosclerosis

참고문헌

  1. Ikeda K, Negishi H, Yamori Y. Antioxidant nutrients and hypoxia/ischemia brain injury in rodents. Toxicology 189: 55-61 (2003) https://doi.org/10.1016/S0300-483X(03)00152-5
  2. Rein D, Yokoyama WH, Xu R, Walzem RL, German JB. Dietary vitamin E in an atherogenic hamster model. Nutr. Res. 18: 567-579 (1998) https://doi.org/10.1016/S0271-5317(98)00043-8
  3. Oh SW, Kwon EK, Kim YE. Screening of angiotensin converting enzyme inhibitory activity in several natural products (abstract no P7-17). In: Abstract: 69th symposium of Food Science and Technology. October 24-26, Hotel Tirol in Muju resort, Muju, Korea. The Korean Society or Food Science and Technology, Seoul. Korea (2002)
  4. Spady DK, Dietschy JM. Interaction of aging and dietary fat in the regulation of low density lipoprotein transport in the hamster. J. Lipid Res. 30: 559-569 (1989)
  5. Kobayashi M, Ishida F, Takahashi T, Taguchi K, Watanabe K, Ohmura I, Kamei T. Preventive effect of MK-733 (simvastatin), an inhibitor of HMG-CoA reductase, on hypercholesterolemia and atherosclerosis induced by cholesterol feeding in rabbits. Jpn. J. Pharmacol. 49: 125-133 (1989) https://doi.org/10.1254/jjp.49.125
  6. Yun YP, Kang WS, Lee MY. The antithrombotic effects of green tea catechins. J. Food Hyg. Safety 11: 77-82 (1996)
  7. Faia KL, Davis WP, Marone AJ, Foxall TL. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in hamster aortic atherosclerosis: correlation with in-situ zymography. Atherosclerosis 160: 325-337 (2002) https://doi.org/10.1016/S0021-9150(01)00590-1
  8. Pitman WA, Osgood DP, Smith D, Schaefer EJ, Ordovas JM. The effect of diet and lovastatin on regression of fatty streak lesions and on hepatic and intestinal mRNA levels for the LDL receptor and HMG CoA reductase in F1B hamsters. Atherosclerosis 138: 43-52 (1998) https://doi.org/10.1016/S0021-9150(97)00302-X
  9. Woollett LA, Spady DK, Dietschy JM. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J. Lipid Res. 33: 77-88 (1992)
  10. Otto J, Ordovas JM, Smith D, Dongen DV, Nicolosi RJ, Schaefer EJ. Lovastatin inhibits diet induced atherosclerosis in F1B golden syrian hamsters. Atherosclerosis 114: 19-28 (1995) https://doi.org/10.1016/0021-9150(94)05457-T
  11. Foxall TL, Shwaery GT, Stucchi AF, Nicolosi RJ, Wong SS. Dose-related effects of doxazosin on plasma lipids and aortic fatty streak formation in the hyperlipidemic hamster model. Am. J. Pathol. 140: 1357-1363 (1992)
  12. Kim YE, Oh SW, Kwon EK, Han D, Kim IH, Lee CH. Effects or green tea, buckwheat and grape leaves extracts in lipid metaboilism, antioxidant capacity and antithrombotic activity in rats fed high cholesterol diets. Korean J. Food Sci. Technol. 36: 979-985 (2004)
  13. La Ville AE, Seddon AM, Shaikh M, Rowles PM, Woolf N, Lewin B. Primary prevention of atherosclerosis by lovastatin in a genetically hyperlipidemic rabbit strain. Atherosclerosis 78: 205-210 (1989) https://doi.org/10.1016/0021-9150(89)90224-4
  14. KNSO. The expectancy of future population. Korea National Statistical Office, Seoul, Korea (2000)
  15. Kwon EK, Oh SW, Lee CH, Han D. Screening of HMG-CoA reductase inhibitory activity in several natural products (ahstract no P7-32). In: Abstract: 70th Nutrigenomics Symposium: Recent trends in food science and technology. June 26-28, Gyeongju TEMF Hotel, Gyeongju, Korea. The Korean Society of Food Scicnce and Technology, Seoul, Korea (2003)
  16. Kowala MC, Nunnari JI, Durham SK, Nicolosi RJ. Doxazosin and cholestyramine similarly decrease fatty streak formation in the aortic arch of hyperlipidemic hamsters. Atherosclerosis 91: 35-49 (1991) https://doi.org/10.1016/0021-9150(91)90185-6
  17. Liu CH, Huang MT, Huang PC. Sources of triglycerol accumulation in liver of rats fed a cholesterol supplemented diets. Lipids 30: 527-531 (1995) https://doi.org/10.1007/BF02537027
  18. Stephanie B, Sabrina SI, Bernard F, Guylene P, Alain P, Laurence B. Effect of ischemia on TBARS and lactate production in several cerebral regions of anaesthetised and awake rats. Life Sci. 74: 3103-3113 (2004) https://doi.org/10.1016/j.lfs.2003.11.013
  19. Bravo E, Cantafora A, Calcabrini A, Ortu G. Why prefer the golden Syrian hamster (Mesocricetus auratus) to the Wister rat in experimental studies on plasma lipoprotein metabolism? Comp. Biochcm. Physiol. 107B: 347-355 (1994)
  20. KNSO. Annual report on the cause of death statistics. Korea National Statistical Office, Seoul, Korea (2003)
  21. Woollett LA, Spady DK, Dietschy JM. Mechanisms by which saturated triacylglycerols elevate the plasma low density lipoprotein-cholesterol concentration in hamsters: Differential effects of fatty acid chain length. J. Clin. Invest. 84: 119-128 (1989) https://doi.org/10.1172/JCI114131
  22. Endres M. Ischemia and stroke. Vol. 513, pp. 455-468. In: Advances in experimental medicine and biology. Dirnagl U(ed). Plenum Press, Inc., NY, USA (2002)
  23. Hadjiisky P, Hermier D, Truffert J, De Gennes JL, Grosgogeat Y. Effect of pravastatin, a HMG-CoA reductase inhibitor, on blood lipids and aortic lipidosis in cholesterol-fed White Carneau pigeons. Biochim. Biophys. Acta 1181: 279-286 (1993) https://doi.org/10.1016/0925-4439(93)90033-W
  24. Lee HK. The nutrition ploblems of Koreans. Korean J. Nutr. 29: 381-383 (1996)
  25. Adams CWM, Bayliss OB. Lipid Histochemistry. Vol. 2, pp. 99-156. In: Techniques of Biochemical and Biophysical Morphology. Glick D. (ed). Wiley, NY, USA (2002)
  26. Rosenfeld L. Lipoprotein analysis. Arch. Pathol. Lab. Med. 113: 1101-1110 (1989)
  27. Spady DK, Dietschy JM. Interaction of dietary cholesterol and triglyceride in the regulation of hepatic low density lipoprotein transport in the hamster. J. Clin. Invest. 81: 300-309 (1988) https://doi.org/10.1172/JCI113321
  28. Nistor A, Bulla A, Filip DA, Radu A. The hyperlipidemic hamster as a model or experimental atherosclerosis. Atherosclerosis 68: 159-173 ( 1987) https://doi.org/10.1016/0021-9150(87)90106-7
  29. Uehara Y, Urata H, Ideishi M, Arakawa K, Saku K. Chymase inhibition suppresses high-cholesterol diet-induced lipid accumulation in the hamster aorta. Cardiovasc. Res. 55: 870-876 (2002) https://doi.org/10.1016/S0008-6363(02)00458-3