Physiological Activities of Peel of Jeju-indigenous Citrus sunki Hort. Tanaka

제주자생 진귤(Citrus sunki Hort. Tanaka) 과피의 생리활성

  • Kang, Shin-Hae (Technology Innovation Center for Life Science, Cheju National University) ;
  • Lee, Young-Jae (Department of Verterinary Medicine, Cheju National University) ;
  • Lee, Chang-Hong (Center for Effective Microorganisms) ;
  • Kim, Se-Jae (Technology Innovation Center for Life Science, Cheju National University) ;
  • Lee, Dae-Ho (Department of Medicine, Cheju National University) ;
  • Lee, Young-Ki (Department of Medicine, Cheju National University) ;
  • Park, Deok-Bae (Department of Medicine, Cheju National University)
  • Published : 2005.12.31

Abstract

Effects of Citrus sunki peel and its fermented product extracts on physiological and functional activities of cellular systems were investigated. Ethanol extract of Citrus sunki peel showed potent ROS-scavenging activity using 2',7'-Dichlorofluorescin diacetate as a fluorescent ROS probe in HepG2 cells. Fermented product of C. sunki peel extract markedly suppressed nitric oxide production in lipopolysaccharide (LPS)-activated RAW264.7 murine macrophage cells. Treatment with fermented product of C. sunki peel extract decreased intracellular protein levels of inducible nitric oxide synthase and cyclooxygenase II stimulated by LPS. High doses of fermented product lend to apoptotic cell death in CHO-IR cells.

Keywords

Citrus sunki;fermentation;ROS;inflammation;apoptosis

References

  1. Guengerich FP, Kim DM. In vitro inhibition of dihydropyridine oxidation and aflatoxin B1 activation in human liver microsomes by naringenin and other flavonoids. Carcinogenesis 11: 2275-2279 (1990) https://doi.org/10.1093/carcin/11.12.2275
  2. Yoo KM, Hwang IK. In vitro effect of Yuza (Citrus junos SIEB ex TANAKA) extracts on proliferation of human prostate cancer cells and antioxidant activity. Korean J. Food Sci. Technol. 36: 339-344 (2004)
  3. Schaus R. Griess' nitrite test in diagnosis of urinary infection. J. Am. Med. Assoc. 161: 528-529 (1956) https://doi.org/10.1001/jama.1956.62970060009009d
  4. Ryu MR, Kim EY, Bae IY, Park YK. Contents of naringin, hesperidin, and neohesperidin in premature Korean Citrus fruits. Korean J. Food Sci. Technol. 34: 132-135 (2002)
  5. Baik SO, Bock JY, Chun HJ, Jeong SI, Baek SH, Oh HB, Kim IK. Analysis and quantitative distribution of glycosided flavonoids in Citruses and Korean Chung-pi. Analytical Sci. Technol. 14: 340-348 (2001)
  6. Park D, Pandey SK, Maksimova E, Kole S, Bernier M. Akt-dependent antiapoptotic action of insulin is sensitive to farnesyl-transferase inhibitor. Biochemistry 39: 12513-12521 (2000) https://doi.org/10.1021/bi000995y
  7. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 (1970) https://doi.org/10.1038/227680a0
  8. Francis AR, Shetty TK. Modulating effect of plant flavonoids on the mutagenecity of n-methyl-n-nitro-n-nitrosoguanidine. Carcinogenesis 10: 1953-1955 (1989) https://doi.org/10.1093/carcin/10.10.1953
  9. Guthrie N, Carroll KK. Inhibition of mammary cancer by citrus flavonoids. pp. 227-236. In: Flavonoids in the living system. Manthey JA, Buslig BS (eds), Plenum. New York, NY, USA (1998)
  10. Cha JY, Cho YS. Biofunctional activities of Citrus flavonoids. J. Korean Soc. Agric. Chem. Biotechnol. 44: 122-128 (2001)
  11. Kim CJ. Screening of physiologically active substance. Jayou Academy, Seoul, Korea. pp. 325-338 (1996)
  12. Chung SK, Kim SH, Choi YH, Song EY, Kim SH. Status of citrus fruit production and view of utilization in Cheju. Food Ind. Nutr. 5: 42-52 (2000)
  13. Chen YQ, Zhou YQ, Wang MH. Activation of the RON receptor tyrosine kinase protects murine macrophages from apoptotic death induced by bacterial lipopolysaccharide. J. Leukoc. Biol. 71: 359-366 (2002)
  14. Moon SW, Kang SH, Jin YJ, Park JG, Lee YD, Lee YK, Park DB, Kim SJ. Fermentation of Citrus unshiu Marc. and functional characteristics of the fermented products. Korean J. Food Sci. Technol. 36: 669-676 (2004)
  15. Kim HY. Distribution, taxonomy, horticultural characters of the local Citrus spp. in Cheju, and the genetic markers among them. PhD thesis, Cheonnam National University, Cheonnam, Korea (1988)
  16. Kang SH, Song JH, Kang HK, Kim SJ, Lee YK, Park DB. Insulin can block apoptosis by decreasing oxidative stress via phosphatidylinositol 3-kinase- and extracellular signal-regulated protein kinase-dependent signaling pathways in HepG2 cells. Eur J. Endocrinol. 148: 147-155 (2003) https://doi.org/10.1530/eje.0.1480147
  17. Blios BS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199 (1958) https://doi.org/10.1038/181958a0
  18. AOAC. Official Methods of AOAC Intl. 15th ed. Method 994. Association of Official Analytical Chemists. Washington, DC, USA (1990)