Differentiation of Four Major Gram-negative Foodborne Pathogenic Bacterial Genera by Using ERIC-PCR Genomic Fingerprinting

ERIC-PCR genomic fingerprinting에 의한 주요 식중독 그람 음성 세균 4속의 구별

  • Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Seo, Hyeon-A (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Young-Joon (Department of Food Science and Technology, Chung-Ang University) ;
  • Cho, Joon-Il (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Sung-Soo (Cheju Traditional Food Institute, Cheju Halla College) ;
  • Song, Dae-Sik (Shinwon Food Industry Co., Ltd.) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
  • 정혜진 (중앙대학교 식품공학과) ;
  • 박성희 (중앙대학교 식품공학과) ;
  • 서현아 (중앙대학교 식품공학과) ;
  • 김영준 (중앙대학교 식품공학과) ;
  • 조준일 (중앙대학교 식품공학과) ;
  • 박성수 (제주한라대학 제주향토식품연구소) ;
  • 송대식 ((주)신원 FI) ;
  • 김근성 (중앙대학교 식품공학과)
  • Published : 2005.12.31

Abstract

Widespread distributions of repetitive DNA elements in bacteria genomes are useful for analysis of genomes and should be exploited to differentiate food-borne pathogenic bacteria among and within species. Enterobacterial repetitive intergenic consensus (ERIC) sequence has been used for ERIC-PCR genomic fingerprinting to identify and differentiate bacterial strains from various environmental sources. ERIC-PCH genomic fingerprinting was applied to detect and differentiate four major Gram-negative food-borne bacterial pathogens, Esherichia coli, Salmonella, Shigella, and Vibrio. Target DNA fragments of pathogens were amplified by ERIC-PCR reactions. Dendrograms of subsequent PCR fingerprinting patterns for each strain were constructed, through which relative similarity coefficients or genetic distances between different strains were obtained numerically. Numerical comparisons revealed ERIC-PCR genotyping is effective for differentiation of strains among and within species of food-borne bacterial pathogens, showing ERIC-PCR fingerprinting methods can be utilized to differentiate isolates from outbreak and to determine their clonal relationships among outbreaks.

Keywords

foodborne pathogenic bacteria;ERIC-PCR;genotyping;genetic similarity

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