Development of Predictive Growth Model of Imitation Crab Sticks Putrefactive Bacteria Using Mathematical Quantitative Assessment Model

수학적 정량평가모델을 이용한 게맛살 부패균의 성장 예측모델의 개발

  • Moon, Sung-Yang (Ourhome Co., Ltd. Food Research Institute Analysis and Inspection) ;
  • Paek, Jang-Mi (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • Shin, Il-Shik (Faculty of Marine Bioscience & Technology, Kangnung National University)
  • 문성양 ((주)아워홈 식품연구원) ;
  • 백장미 (강릉대학교 해양생명공학부) ;
  • 신일식 (강릉대학교 해양생명공학부)
  • Published : 2005.12.31

Abstract

Predictive growth model of putrefactive bacteria of surimi-based imitation crab in the modified surimi-based imitation crab (MIC) broth was investigated. The growth curves of putrefactive bacteria were obtained by measuring cell number in MIC broth under different conditions (Initial cell number, $1.0{\times}10^2,\;1.0{\times}10^3$ and $1.0{\times}10^4$ colony forming unit (CFU)/mL; temperature, $15^{\circ}C,\;20^{\circ}C\;and\;25^{\circ}C$) and applied them to Gompertz model. The microbial growth indicators, maximum specific growth rate constant (k), lag time (LT) and generation time (GT), were calculated from Gompertz model. Maximum specific growth rate (k) of putrefactive bacteria was become fast with rising temperature and fastest at $25^{\circ}C$. LT and GT were become short with rising temperature and shortest at $25^{\circ}C$. There were not significant differences in k, LT and GT by initial cell number (p>0.05). Polynomial model, $k=-0.2160+0.0241T-0.0199A_0$, and square root model, $\sqrt{k}=0.02669$ (T-3.5689), were developed to express the combination effects of temperature and initial cell number, The relative coefficient of experimental k and predicted k of polynomial model was 0.87 from response surface model. The relative coefficient of experimental k and predicted k of square root model was 0.88. From above results, we found that the growth of putrefactive bacteria was mainly affected by temperature and the square root model was more credible than the polynomial model for the prediction of the growth of putrefactive bacteria.

Keywords

predictive growth model;putrefactive bacteria;polynomial model;square root model;maximum specific growth rate constant (k)

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