Simultaneous Determination of Vitamin $D_3\;and\;K_1$ in Infant Formula by Column-switching High Performance Liquid Chromatography with UV Detection

Column-switching HPLC를 이용한 성장기용 조제식 중 비타민 $D_3,\;K_1$의 동시분석

  • Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Ahn, Jang-Hyuk (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Chang, Chi-Hoon (Research and Development Center, Namyang Dairy Products Corporation)
  • 곽병만 (남양유업(주) 중앙연구소) ;
  • 안장혁 (남양유업(주) 중앙연구소) ;
  • 장치훈 (남양유업(주) 중앙연구소)
  • Published : 2005.12.31

Abstract

Rapid and simple method was developed for simultaneous determination of vitamins $D_3\;and\;K_1$ contents in infant formula. Contents of vitamins $D_3\;and\;K_1$, extracted by column-switching HPLC with reversed phase column using enzymatic hydrolysis and organic solvent, in CRM determined by developed method were within certified ranges of standard values.

Keywords

enzymatic hydrolysis extraction;column-switching HPLC;simultaneous determination

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