Capillary Electrophoretic Method for the Determination of (+)-Catechin, (-)-Epicatechin in Grape Seed Ethanol Extract

포도종실 에탄올 추출물에 함유된 (+)-카테킨, (-)-에피카테킨의 모세관 전기영동법에 의한 분석

  • Choi, One-Kyun (Department of Food Science & Biotechnology and Food & Bio-industrial Research Center, Hankyong National University) ;
  • Chung, Yang-Seop (Department of Food Science & Biotechnology and Food & Bio-industrial Research Center, Hankyong National University) ;
  • Chung, Ha-Yull (Department of Food Science & Biotechnology and Food & Bio-industrial Research Center, Hankyong National University)
  • 최원균 (한경대학교 식품생물공학과 및 식품생물산업연구소) ;
  • 정양섭 (한경대학교 식품생물공학과 및 식품생물산업연구소) ;
  • 정하열 (한경대학교 식품생물공학과 및 식품생물산업연구소)
  • Published : 2005.08.31

Abstract

Capillary electrophoresis (CE) method was developed to determine (+)-catechin and (-)-epicatechin contents in grape seed ethanol extract. CE separation was achieved using 100 mM phosphate and borate buffer at pH 6.0 as background electrolyte and fused silica capillary with 50 microns x 375 microns O.D. (effective length 20.0cm) maintained at $25^{\circ}C$. The applied voltage was 10kV, and detection was performed by DAD at 210 nm, Two catechins were well separated within 6 min with repeatability of <0.8% RSD for migration time and <2.0% RSD for peak area, and correlation coefficients higher than 0.994 were obtained from 58.0 to 174.0 mg/L with detection limit of 0.035 mg/L. Separated compounds were successfully determined. CE method was easy to handle and showed good reproducibility. CE method was compared with conventional coloring and HPLC methods, and main advantages of CE method were low amount of sample required, simple pre-sample treatment, good recovery rate, and short analysis time.

Keywords

capillary electrophoresis;catechin;epicatechin;grape seed ethanol extract

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