Antioxidant and Anticancer Activities of Extracts from Styela plicata

주름 미더덕(Styela plicata) 추출물의 항산화력 및 항암활성

  • Kim, Jin-Ju (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Jung (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Hee (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
  • 김진주 (경남대학교 식품생명공학부) ;
  • 김선정 (경남대학교 식품생명공학부) ;
  • 김선희 (경남대학교 식품생명공학부) ;
  • 박해룡 (경남대학교 식품생명공학부) ;
  • 이승철 (경남대학교 식품생명공학부)
  • Published : 2005.08.01


The antioxidant and anticancer activities of Styela plicata extracts were evaluated. When extracts were prepared with fresh Styela plicata (FR), extraction yield was in the order of methanol > ethanol = acetone > water among treated solvents. However, the extraction order was methanol > water > ethanol > acetate in freeze dried Styela plicata (FD). Radical scavenging activity was the highest in acetone extracts $(37.39\%)$ from FR, while in ethanol extracts $(78.40\%)$ from FD. Reducing power of FR was the greatest in methanol extracts (1.076), and that of FD in ethanol extracts (1.360). The acetone extracts from FD showed significant anticancer activity when revealed with human colon cancer cell line HT-29. These results indicated that extraction yields and properties of extracts from Styela plicata were variable depending on solvent and/or physicochemical state, and appropriate extraction process could provide some valuable bioactive materials from Styela plicata.


  1. Jo YG. 1978. The sterol composition of Styela clava. Kor Fish Soc 11: 97-101
  2. Lee KH, Park CS, Hong BI, Jung BC, Cho HS, Jea YG. 1995. Seasonal variations of nutrients in warty sea squirt (Styela clava). J Korean Soc Food Nutr 24: 268-273
  3. Ahn SH. 2003. Extraction of glycosaminoglycans from Styela clava tunic. Biotechnol Bioproc Eng 18: 180-185
  4. Lehrer RI. 2001. Clavanins and styelins, alpha-helical antimicrobial peptides from the hemocytes of Styela clava, Adv Exp Med Biol 484: 71-76
  5. Menzel LP, Lee IH, Sjostrand B, Lehrer RI. 2002. Immunolocalization of clavanins in Styela clava hemocytes, Dev Comp Immunol 26: 505-515
  6. Lee IH, Zhao C, Nguyen T, Menzel L, Waring AJ, Sherman MA, Lehrer RI. 2001. Clavaspirin, an antibacterial and haemolytic peptide from Styela clava. J Pept Res 58: 445-456
  7. Taylor SW, Craig AG, Fischer WH, Park M, Lehrer RI. 2000. Styelin D, an extensively modified antimicrobial peptide from ascidian hemocytes. J Bioi Chem 275: 38417-38426
  8. Blake D, Winyard PG. 1995. Immunopharmacology of free radical species. Academic Prss, San Diego
  9. Lopaczynski W, Zeissel SH. 2001. Antioxidant, programmed cell death, and cancer. Nutr Res 21: 295-307
  10. Cook NC, Samman S. 1996. Flavonoids chemistry, mechanism, cardioprotective effects and dietary source. Nutr Biochem 7: 66-76
  11. Namiki M. 1990. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29: 273-300
  12. Gerlier D, Thomasset N. 1986. Use of MTT colorimetric assay to measure cell activation. J Immun Meth 94: 57-63
  13. Kang TB, Liang NC. 1997. Studies on the inhibitory effects of quercetin on the growth of HL -60 leukemia cells. Biochem Pharm 54: 1013-1018
  14. Park KY, Moon SH, Rhee SH, Baek KY, Lim SY. 1995. Effect of tannin from persimmon leaves on the growth inhibition and the synthesis of mRNA of type IV collagen in AZ-521 human gastric cancer cells. Environm Muta Carcino 15: 32-37
  15. Park JC. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogen 27: 117-122
  16. Lee SC, Kim JH, Jeong SM, Kim DR, Ha JU, Nam KC, Ahn DU. 2003. Effect of far-infrared radiation on the antioxidant activity of rice hulls. J Agric Food Chem 51: 4400-4403
  17. Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307-315
  18. National Statistical Office. 2001. Annual Report on the Cause of Death Statistics. Seoul, Korea
  19. Goodman GY, Yen YP, Cox TC, Crowlny J. 1987. Effect of verapamilon in vitro cytotoxicin and vinblastin in human tumor cell. Cancer Res 47: 2295-2311
  20. Fish B. 1984. Clinical trials for the evaluation of cancer therapy. Cancer Res 54: 609-615
  21. SAS Institute. 1995. SAS/STAT User's Guide. SAS Institute Inc., Cary, NC
  22. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1 -diphenyl-2-picrylhydrazyl radical. Biochem Pharm 56: 213-222
  23. Bondent V, Brand-Williams W, Bereset C. 1997. Kinetics and mechanism of antioxidant activity using the DPPH free radical methods. Lebensm- Wiss Technol 30: 609-615
  24. Sato A, Shindo T, Kasanuki N, Hasegawa K. 1989. Antioxidant metabolites from the tunicate Amaroucium multiplicatum. J Nat Prod 52: 975-981
  25. Matsuno T, Ookubo M, Komori T. 1985. Carotenoids of tunicates. III. The structural elucidation of two new marine carotenoids, amarouciaxanthin A and B. J Nat Prod 48: 606-613
  26. Cotelle N, Moreau S, Bernier JL, Catteau JP, Henichart JP. 1991. Antioxidant properties of natural hydroquinones from the marine colonial tunicate Aplidium californicurn. Free Radic BioI Med 11: 63-68
  27. Choi BD, Kang SJ, Choi YJ, Youm MG, Lee KH. 1994. Utilization of ascidian (Halocynthia roretzi) tunic. 3. Carotenoid compositions of ascidian tunic. Bull Korean Fish Soc 27: 344-350
  28. El-Agarney A, Lowe GM, McGarvey DJ, Mortensen A, Phillip DM, Truscott TG, Young AJ. 2004. Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys 430: 37-48
  29. Mier S, Kanner J. Akiri B, Hadas SP. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43: 1813-1819
  30. Diplock AT. 1997. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad Res 27: 511-532
  31. Jung BM, Lim SS, Park YJ, Bae SJ. 2005. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions on human cancer cells. J Korean Soc Food Sci Nutr 34: 8-12
  32. Choe WK, Park JH, Kim SH, Lee DY, Lee YC. 1999. Antitumor effects of green tea catechin on different cancer cells. Korean J Nutr 32: 838-843

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