Protective Effect of Saengshik Supplementation on Lead Induced Toxicity in Rats

생식의 섭취가 납중독 흰쥐의 피해 경감에 미치는 영향에 관한 연구

  • Oh, Young-Joo (R&D Center for Functional Foods, Institute of Food and Culture, Pulmuone Co. Ltd.,) ;
  • Kim, Jae-Min (Preclinical Research Center, ChemOn Inc.,) ;
  • Song, Si-Wan (Preclinical Research Center, ChemOn Inc.,) ;
  • Ha, Hyo-Cheol (R&D Center for Functional Foods, Institute of Food and Culture, Pulmuone Co. Ltd.,) ;
  • Kim, Hyun-Su (R&D Center for Functional Foods, Institute of Food and Culture, Pulmuone Co. Ltd.,)
  • 오영주 (풀무원식문화연구원 기능성연구소) ;
  • 김재민 (㈜켐온 전임상연구센터) ;
  • 송시환 (㈜켐온 전임상연구센터) ;
  • 하효철 (풀무원식문화연구원 기능성연구소) ;
  • 김현수 (풀무원식문화연구원 기능성연구소)
  • Published : 2005.08.01


Lead is a ubiquitous environmental and industrial pollutant that causes a major health concerns. It is known to induce a broad range of physiological, biochemical, and behavioral dysfunctions in laboratory and humans, including hematopoietic system, kidneys, liver, and reproductive system. This study was conducted to investigate the effect of Saengshik supplementation on the lead-induced toxicity in rats. Five week old male Sprague­Dawley rats were randomly assigned to five groups for six weeks as followings: control group (CT), lead acetate treated group (PT), and lead acetate groups administered with three different dosages of Saengshik $(SI2.5-12.5\%,\;S25-25\%,\;and\;S50-50\%).$ Lead acetate (12 mg/rat) was intragastrically administered daily for 6 weeks. The results were summarized as follows; Weight gain and food efficiency ratio were significantly lower (p<0.05) in lead administered group compared with those of the control group. Also, significant lead-induced alteration in blood hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and reticulocyte distribution width (RDW) were observed. In the liver of lead-exposed animals, there was an increase in the lipid peroxidation (MDA) and the level of glutathione (GSH), but superoxiede dismutase (SOD) activity did not change. Lead-exposed animals with $25\%\;and\;50\%$ Saengshik supplementation showed marked improvements in the values of MCH, MCV, and RDW. Also, the level of HCT was significantly increased by $50\%$ Saengshik supplementation. The levels of liver MDA in $12.5\%\;and\;50\%$ Saengshik administered groups and GSH level in $50\%$ Saengshik administered group were significantly decreased compared to the lead administered group. Also, hepatic SOD activity tended to increase in the presence of Saengshik supplementation. Furthermore, the accumulation of lead in liver and kidney was reduced by presence of Saneghshik supplementation. Liver lead concentration was significantly reduced by both $25\%\;and\;50\%$ Saengshik supplementations and kidney lead concentration was significantly reduced by the $25\%$ Saengshik supplementation. These results show that Saengshik may have a protective effect against lead intoxication but the mechanism of their effects remains unclear.


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