Effects of Chitosan on the Induction of Renal Dipeptidase (RDPase) from the Proximal Tubules

신장의 근위세뇨관에서 Renal Dipeptidase(RDPase)의 유도에 관한 키토산의 효과

  • Kim, Young-Ho (Dept. of Natural Science, Biology, Chosun University, College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Yoon, Hyun-Joong (College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Park, Haeng-Soon (College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Lee, Myung-Yul (Dept. of Food and Nutrition, Chosun University) ;
  • Kim, Jong-Se (Dept. of Natural Science, Biology, Chosun University)
  • 김영호 (조선대학교 자연과학대학 생물학과, 전남대학교 약학대학 약품개발연구소) ;
  • 윤현중 (전남대학교 약학대학 약품개발연구소) ;
  • 박행순 (전남대학교 약학대학 약품개발연구소) ;
  • 이명렬 (조선대학교 자연과학대학 식품영양학과) ;
  • 김종세 (조선대학교 자연과학대학 생물학과)
  • Published : 2005.08.01


The purpose of this study was to evaluate the effects of chitosan, which is deacetylated derivative of chitin, on the renal function. Renal dipeptidase (RDPase, membrane dipeptidase, dehydropeptidase 1, EC is glycosyl phosphatidyl-inositol (GPI)-anchored ectoenzyme of renal proximal tubular microvilli and was related with renal disease including acute renal failure, pyelitis and nephritis. The released RDPase and Udpase activities were assayed by modified fluorometric method. In vitro experimental groups were consisted of group 1, the concentration ranges of 0, 0.01, 0.05 and $0.1\%$ chitosan only, group 2, the concentration ranges of 1, 2 and 4 mM glycerol only, and group 3, the concentration ranges of 0, 0.01, 0.05 and $0.1\%$ chitosan in the presence of glycerol (4 mM). In vivo experimental groups were consisted of group 1 in which rats were treated with glycerol for the purpose of glycerol-induced renal damage, and group 2 in which rats were treated with chitosan plus glycerol. The RDPase release of 0.01, 0.05, and $0.1\%$ chitosan groups were increased in the concentration dependent manner. The RDPase release of 1, 2, and 4mM glycerol groups were decreased in the concentration dependent manner. Chitosan in the presence of glycerol restored the released RDPase activity in the proximal tubules. In vivo, chitosan inhibited the decrease of RDPase release by glycerol in the kidney and blocked the decrease of Udpase activity by glycerol in urine. These results indicated that chitosan was possible as a functional food to control renal function and its diseases.


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