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솔잎증류액의 투여가 Streptozotocin으로 유도한 당뇨쥐에서 혈당, 구강내당능검사, 혈액 성분에 미치는 영향

Effect of Pinus densiflora Extract on Blood Glucose Level, OGTT and Biochemical Parameters in Streptozotocin Induced Diabetic Rats

  • 김신희 (충남대학교 식품영양학과) ;
  • 황석연 (충북대학교병원 임상병리과) ;
  • 박오성 (충남대학교 수의학과) ;
  • 김무강 (충남대학교 수의학과) ;
  • 정영진 (충남대학교 식품영양학과)
  • Kim, Shin-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Hwang, Seock-Yeon (Dept of Clinical Laboratory, Chungbuk National University Hospital) ;
  • Park, Oh-Sung (Dept. of Veterinary Medicine, Chungnam National University) ;
  • Kim, Moo-Kang (Dept. of Veterinary Medicine, Chungnam National University) ;
  • Chung, Young-Jin (Dept. of Food and Nutrition, Chungnam National University)
  • 발행 : 2005.08.01

초록

한국에서 자생하는 흑송의 솔잎을 재료로 하여 항당뇨효능에 대한 생리 활성을 규명하고자 STZ로 당뇨를 유발시킨 흰쥐 에 솔잎증류액을 15일간 복강투여 후 체중, 혈당, OGTT, 혈액성분에 미치는 영향 및 간과 췌장의 조직병리학적 변화를 관찰하였다. 솔잎 투여군의 체중은 솔잎투여 6일째에 잠시 체중의 상승이 나타났을 뿐 그 이후에는 DM군에서와 같이 체중의 증가를 보이지 않았다. 공복혈당은 DM군에 비해 솔잎투여군에서 용량효과적으로 낮아지는 경향을 보였으며 15일째에 HP군에서 NC군의 수준으로 근접하였다. 구강내당능 검사결과 혈당곡선은 DM군에 비해 통계적 유의성은 없었으나 솔잎투여군에서 투여용량에 따라 감소경향을 보였다. 간기능관련지수로서 혈장내 total protein, albumin, AST는 솔잎투여에 따른 변화가 없었고, ALP와 ALT는 솔잎투여군 모두에서 DM군에 비해 감소하는 경향을 나타내었다. 신장기능 관련지수에서는 creatinine, uric acid, calcium, IP는 개선 경향이 관찰되지 않았으나, BUN에서는 DM과 비교시 솔잎투여군 모두에서 용량효과적으로 개선되는 경향을 보였다. 지질대사관련지수로서 total cholesterol, TG, HDL-C, LDL-C 농도는 모든 실험군간에 유의적인 차이를 보이지 않았다. 간 조직과 췌장조직 병리학적 관찰결과 간조직의 경우 DM군과 솔잎투여군의 일부에서 염증세포의 침윤이 부분적으로 관찰되었고 췌도세포에서는 DM군은 췌장실질의 괴사, 출혈성 병변, 지방세포의 괴사가 관찰되었으나 솔잎투여군 모두에서 DM군에 비해 억제되는 현상을 관찰할 수 있었다. 결론적으로 솔잎추출물 투여 에 의 괘 공복혈당이 낮아지고 당내성이 높아지는 경향을 보였고 간기능 지표 중 ALP와 ALT, 신장기능지표 중 BUN만이 솔잎 투여에 의한 개선효과를 나타내었으며, 혈중 지질 개선효과는 나타내지 않았다.

참고문헌

  1. Gonuth SM. 1973. Plasma insulin and glucose profiles in normal, obese and diabetic persons. Ann Intern Med 79: 812-817 https://doi.org/10.7326/0003-4819-79-6-812
  2. Abrams JJ, Ginsberg H, Grundy SM. 1982. Metabolism of cholesterol and plasma triglycerides in nonketotic diabetes mellitus. Diabetes 31: 903-910 https://doi.org/10.2337/diabetes.31.10.903
  3. Kannel WB, McGee DL. 1979. Diabetes and cardiovascular disease. JAMA 241: 2035-2038 https://doi.org/10.1001/jama.241.19.2035
  4. Bailey CJ, Day C. 1989. Traditional plant medicines as treatments for diabetes care. Diabetes Care 12: 553-564 https://doi.org/10.2337/diacare.12.8.553
  5. Choi JS, Chung HY, Han SY. 1990. A preliminary study on hypercholesterolemic and hyperglycemic activities of some medical plants. Korean J Pharm 21: 153-157
  6. Kim TH, Yang KS, Whang SH. 1990. Studies on the physiochemical activities of Commelinae herba extract on the normal and streptozotocin-induced hyperglycemic rats. Thesis collection. SM Pharm Sci 7: 39-59
  7. Lim SJ, Kim SY, Lee JW. 1995. The effects of Korean wild vegetables on blood glucose level and liver-muscle metabolism of streptozotocin-induced diabetic rats. Korean J Nutr 28: 585-594
  8. Hikino H, Takahashi M, Oshima Y, Konno C. 1998. Isolation and hyperglycemic activity of oryzabrans A, B, A and D, glycans of Oryza sativa bran. Planta Medica 54: 1-3
  9. Lee MY, Lee JS, Sheo HJ. 1988. Effects of Aralia elata extract on experimentally alloxan induced diabetes in rabbits. J Korean Soc Food Nutr 17: 57-61
  10. Sheo HJ, Jun SJ, Lee MY. 1986. Effects of Lycii fructus extract on experimentally induced liver damage and alloxan diabetes in rabbits. J Korean Soc Food Nutr 15: 136-143
  11. Kim MH. 1995. Hypoglycemic effect of Dioscorea japonica thunb in streptozotocirr-induced diabetic rats. PhD Dissertation. Duksung Women's University
  12. Lee JS, Son HS, Maeng YS, Chang YK, Ju JS. 1994. Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J Nutr 27: 819-827
  13. Hsu FL, Liu IM, Kuo DH, Chen WC, Su HC, Cheng JT. 2003. Antihyperglycemic effect of puerarin in streptozotocin-induced diabetic rats. J Nat Prd 66: 788-792 https://doi.org/10.1021/np0203887
  14. 고경식, 1991, 한국식물검색도감, 아카데미출판사, 서울, p.16
  15. 김완희, 1983, 최신 동의보감, 태평양출판공사, 서울, p.960
  16. Choi JH, Kim DI, Park SH, Kim DW, Lee JH, Kim HS. 2001. Investigation of anti-aging effect and determination of chemical structure of pine needle (Pinus densiflora) through animal experiment III. Effects of butanol fraction on oxygen radicals and their scavenger enzymes-in brain of SD rats. Korean J Gerontol 11: 7-13
  17. Choi JH, Kim DI, Park SH, Kim CM, Kim SH. 2001. Investigation of anti-aging effect and determination of chemical structure of pine needle (Pinus densiflotu) through animal experiment N. Effects of ethyl acetate fraction on oxygen radicals and their scavenger enzymes in liver of SD rats. Korean J Gerontol 11: 14-20
  18. Kim EJ, Jung SW, Choi KP, Ham SS. 1998. Cytotoxic effect of the pine needle extracts. Korean J Food Sci Technol 30: 213-217
  19. Kong Z, Lui Z, Ding B. 1995. Study on antimutagenic effect of pine neddle extract. Mutat Res 347: 101-104 https://doi.org/10.1016/0165-7992(95)00026-7
  20. Choi EJ, Lee E, Rhin TJ, Cha BC, Park HJ. 1997. Anti-microbial activities of pine needle (Pinus densiflora) extract. Korean J Appl Microbial Biotechnol 25: 293-297
  21. Kim JD, Yoon TH, Choi M, Lim KJ, Ju Js, Lee SY. 1991. Effect of dietary supplementation with pine leaf on lipid parameters in rats. Korean J Gerontol 1: 47-50
  22. Kang YH, Park YK, Ha TY, Moon KD. 1996. Effect of pine niddle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr 25: 367-373
  23. Kang YH, Park YK, Ha TY, Moon KD. 1996. Effect of pine niddle extracts on enzyme activity of serum and liver, liver morphology in rats fed high fat diet. J Korean Soc Food Nutr 25: 374-378
  24. Beppu H, Maruta K, Kruer T, Kolb H. 1987. Diabetogenic action of streptozotocin: essential role of membrane permeability. Acta Endocrinol (Copenh) 114: 90-95
  25. Matkovics B, Kotorman M, Varga IS, Hai DQ, Varga C. 1998. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 85: 29-38
  26. Kahn CR. 1985. The molecular mechanism of insulin actoin. Ann Rev Med 36: 249-251
  27. Choi SS. 1995. (The) effects of trichosanthes kirilouiii max. extract on blood glucose levels and energy metabolism of streptozotocin-induced diabetic rats. PhD Dissertation. Duksung Women's University
  28. Ju JS, Choi M, Koh ES, Choi MG. 1989. Effect of adrenal hormones and diets on diabetic rats. Korean J Nutr 22: 63-69
  29. Bursch W, Schulte HR. 1986. Cytoprotective effect of the prostacyclin derivative iloprost against liver cell death induced by the hepatotoxins carbon tetrachloride and bromobenzene. Klin Wochenschr 64 (suppl, 7): 47-50
  30. Guyton & Hall. 1996. Textbook of medical physiology. 9th ed. W.B. Sanders, Philadelphia. p 346
  31. Park IK. 2000. Biochemistry. 2nd ed. Life Sci, Seoul. p 336
  32. Choi MJ, Han YJ. 1994. Effects of the soy protein level on plasma glucose, lipid, and hormones in streptozotocin-diabetic rats. Korean J Nutr 27: 883-891
  33. Lim SJ, Kim SH. 2001. The effect of each fraction of methanol extract of Alisma canaliculatum on blood glucose levels and lipid metabolism in streptozotocin induced diabetic rats. Korean J Nutr 34: 619-625
  34. O'Meara NMG, Devery RAM, Owens D, Collins PB, Johnson AH, Tomkin GH. 1990. Cholesterol metabolism in alloxan-induced diabetic rabbit. Diabetes 39: 626-633 https://doi.org/10.2337/diabetes.39.5.626
  35. Nikkila EA, Kekki M. 1973. Plasma triglyceride transport kinetics in diabetes mellitus. Metabolism 22: 1-22 https://doi.org/10.1016/0026-0495(73)90024-3

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