Antifungal Activity of Streptomyces griseofuscus 200401 against Pathogens causing Late Blight and Anthracnose on Pepper

역병균과 고추 탄저병에 대한 Streptomyces griseofuscus 200401의 항균활성

  • Lim, Tae-Heon (Technology Innovation Center, Sangju National University)
  • 임태헌 (상주대학교 기술개혁센터)
  • Published : 2005.03.31

Abstract

To select microorganisms that exhibit antifungal activity against the fungal pathogens of pepper, Phytophthora capsici and Colletotrichum acutatum, soil samples from a forest and natural fields of Gajang-Dong, Sangju-city were tested in vitro and in vivo. Streptomyces griseofuscus 200401 was finally selected throughout antifungal activity test with dual culture, culture broth, and fruits. For the identification of the strain, nucleotide sequences of 16S rDNA and whole cell fatty acids were analyzed. It is like that the strain 200401 may be a novel biological control agent that can reduce application of chemical fungicides to control late blight and anthracnose on pepper.

References

  1. Lim, T. H., T. H. Chang and B. J. Cha (1998) Incidence of benzimidazole-and dicarboximide-resistant isolates of Monilinia fructicola from over-wintering mummies and peduncles on peach trees. Korean J. Plant Pathol. 14:367-370
  2. Postermaster, A., J. Kuo, K. Sivasithamparam (1997) Interaction between Colletotrichum musae and antagonistic microorganisms on the surface of banana leaf discs. Scientia Horticulturae 71:113-125 https://doi.org/10.1016/S0304-4238(97)00080-0
  3. Sivan, A. and I. Chet (1989) Degradation of fungal cell wall by lytic enzymes of Trichoderma harzianum. J. Gen. Microbiol. 135:675-682
  4. Trejo-Estrada, S. R., A. Paszczynski and D. L. Crawford (1998) Antibiotics and enzymes produced by biocontrol agent Streptomyces vioaceusniger YCED-9. J. Industrial Microbiology & Biotechnology 21:81-90 https://doi.org/10.1038/sj.jim.2900549
  5. Ahmed, A. S., M. Ezziyyani, P. Sanchez and M. E. Candela (2003) Effect of chitin on biological control activity Bacillus spp. and Trichoderma harzianum against root rot disease in pepper (Capsicum annuum) plant. European J. Plant Pathology 109:633-637 https://doi.org/10.1023/A:1024734216814
  6. Baker, C. J., J. R., Stavely, C. A., Thomas, M. Saser and J. S. MacFall (1983) Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves. Phytopathology 73: 1148-1152 https://doi.org/10.1094/Phyto-73-1148
  7. Karsten, L. and P. Jeffries (2000) Potential for Biological control of diseases caused by Colletotrichum. pp.266-291. In Colletotrichum (ed. Prusky, D., S. Freeman and M. B. P. Dickman), APS Press, Minnesota, U.S.A
  8. Lim, T. H., J. M. Lee, T. H. Chang and B. J. Cha (2000) Antifungal activity and identification of an Actinomycetes strain isolated from mummified peaches. Kor. J. Microbiol. Biotechnol. 28:161-166
  9. Powel, K. A. and F. M. Fox (1993) Technical and commercial aspects of biocontrol produts. Pestic. Sci. 37:315-321 https://doi.org/10.1002/ps.2780370403
  10. Harindran, J., T. E. Gupte and S. R. Naik (1999) HA-1-92, A new antifungal antibiotic produced by Streptomyces CDIL-312: Fermentation, isolation, purification and biological activity. World J. Microbiology & Biochemistry 15:425-430 https://doi.org/10.1023/A:1008934808486
  11. Skujus, J. J., H. J. Potgieter and M. Alexander (1965) Dissolution of fungal cell walls by a Streptomycete chitinase and $\beta$-($1\rightarrow3$) glucanase. Arch. Biochem. Biophys. 111 :358-364 https://doi.org/10.1016/0003-9861(65)90197-9
  12. Ames, B. N. (1979) Identifying environmental chemicals causing mutations and cancer. Science 204:587-593 https://doi.org/10.1126/science.373122
  13. Becker, J. O. (1993) Control of soil-born pathogens with living bacteria and fungi: status and outlook. Pestic. Sci. 37:355-363 https://doi.org/10.1002/ps.2780370408
  14. Mao, W., J. A. Lewis, R. D. Lumsden and K. P. Hebbar (1998) Biocontrol of selected soil borne diseases of tomato and pepper plant. Crop Protection 17:535-542 https://doi.org/10.1016/S0261-2194(98)00055-6
  15. Xiao, K., L. L. Kinkel and D. A. Samac (2002) Biological control of Phytophthora root rots on alfalfa and soybean with Streptomyces. Biological Control 23:285-295 https://doi.org/10.1006/bcon.2001.1015
  16. Taso, P. H. and J. J. Oster (1981) Relation of ammonia and nitrous acid to suppression of Phytophthora in. soils amended with nitrogenous organic substances. Phytopathology 71:53-59 https://doi.org/10.1094/Phyto-71-53
  17. Hiradate, S., S. Yoshida, Y. H. Sugie and Y. Fujii (2002) Muberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2. Phytochemistry 61:693-698 https://doi.org/10.1016/S0031-9422(02)00365-5
  18. Samac, D. A., A. M. Willert, M. J. McBride and L. L. Kinkel (2003) Effects of antibiotic-producing Streptomyces on nodulation and leaf spot in alfalfa. Applied Soil Ecology 22:55-66 https://doi.org/10.1016/S0929-1393(02)00109-9
  19. Hausbeck, M. K. (2004) Phytophthora capsici on vegetable crops: Research progress and management challenges. Plant Disease 88: 1292-1303 https://doi.org/10.1094/PDIS.2004.88.12.1292