Hypoglycemic and Antioxidative Effects of Fermented Chaga Mushroom (Inonotus obliquus) on Streptozotocin-induced Diabetic Rats

Streptozotocin 유발 당뇨쥐에서 발효 차가버섯의 항당뇨 및 항산화 효과

Cha Jae-Young;Jun Bang-Sil;Lee Chi-Hyeoung;Yooi Ki-Soo;Moon Jae-Chul;Cho Young-Su

  • Published : 2005.10.01


The effects of fermented chaga mushroom (Inonotus obliquus) on the concentrations of serum glucose, insulin, lipids and lipid peroxidation in streptozotocin (STZ)-induced diabetic rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (the CM group) and fermented chaga mushroom powder (the FCM group), and no supplemented (the control group) for 3 weeks. The polysaccharide concentrations were CM by $42.9\%$ and FCM by $ 39.1\%$, and the total polyphenol concentrations were CM by $ 0.80\%$ and FCM by $0.91\%$. Feed intakes and water consumption, serum glucose, insulin, triglyceride, and blood urea nitrogen concentrations were significantly lower in the FCM group than in both the CM and control groups. The activities of AST and ALT were also significantly lower in the FCM group than in the control group. No significant differences were detected with regard to the serum cholesterol and creatinine concentrations among the experimental groups. Lipid peroxidations in hepatic homogenate, microsomal and mitochondrial subcellular and pancreas were significantly lowered by the administration of FCM in the STZ-diabetic rats. Hepatic glutathione concentrations, which is closely associated with antioxidant system, was significantly higher in the FCM group than in the control group, indicating a marked effect of FCM administration on the endogenous antioxidant system. However, CM treatment showed a moderate antioxidative activity in the STZ-diabetic rats. Our results indicate that fermented chaga mushroom exert hypoglycemic and antioxidative effects in type 1 diabetes mellitus.


Inonotus obliquus;Chaga mushroom;diabetes mellitus;streptozotocin;hypoglycemia.


  1. Alarcon-Aguilara, F. L R. Roman-Ramos, S. Perez-Gutierrez, A Aguilar-Contreras, C. C. Contreras-Weber and J. J. Flores-Saenz. 1998. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J. Ethnopharmacol. 61, 101-11
  2. AI-Ghaithi, F., M. R. EI-Ridi, E. Adeghate and M. H. Amiri. 2004. Biochemical effects of Citrullus colocynthis in normal and diabetic rats. Mol. Cell. Biochem. 261, 143-149
  3. Awadh-Ali, N. A, R. A A Mothana, A Lesnau, H. Pilgrim and U. Lindequist. 2003. Antiviral activity of Inonotus obliquus hispidus. Fitoterapia. 74, 483-485
  4. Benoit, M. A., G. D. Aprano and M. Lacroix. 2000. Effect of ${\gamma}$-irradiation on phenylalanine ammonia-lyase activity, total phenolic content, and respiration of mushrooms (Agaricus bisporus). J. Agric. Food. Chem. 48, 6312-6316
  5. Berger, A, D. Rein, E. Kratky, J. Monnard, H. Hajjaj, J. Meirim, C. Piguet-Welsch, J. Hau, K. Mace and P. Niederberger. 2004. Cholesterol-lowering properties of Ganoderma lucidum in vitro, ex vivo, an in hamsters and minipigs. Lipid. Health. Disease. 3-12
  6. Beutler, E., O. Duron and B. M. Kelly. 1963. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61, 882-88
  7. Cha, J. Y., B. S. Jeon, J. W. Park, J. C. Moon and Y. S. Cho. 2004. Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells. J. Korean. Soc. Appl. Biol. Chem. 47, 202-207
  8. Cha, J. Y., B. S. Jeon, J. W. Park, G. G. Shin, B. K. Kim, D. W. Bae, J. H. Yoo, B. S. Jeon and Y. S. Cho. 2004. Antioxidative effects of mushroom extract and fermented milk containing its extract on in vivo and in vitro lipid peroxidation. J. Life. Sci. 14, 514-520
  9. Cha, J. Y., B. S. Jeon, J. W. Park, G. G. Shin, B. K. Kim, H. K. Kim and Y. S. Cho. 2004. Hypoglycemic effect of mushroom fermented milk in streptozotocin induced diabetic rats. J. Life. Sci. 14, 676-682
  10. Chang, S. T. 1999. Wrold production of cultivated edible and medicinal mushrooms in 1977 with emphasis on Lintinus edodes (Berk) Sig. in China. Int. J. Med. Mushrooms. 1, 291-300
  11. Coseteng, M. Y. and C. Y. Lee. 1987. Changes in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning. J. Food. Sci. 52, 985-989
  12. Duncan, D. B. 1957. Multiple range test for correlated and heteroscedastic means. Biometrics. 13, 164-176
  13. Fukushima, M., T. Ohashi, Y. Fujiwara, K. Sonoyama and M. Nakano. 2001. Cholesterol-lowering effects of maitake (Grofola frondosa) fiber, shiitake (Letinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp. Biol. Med. 226, 758-765
  14. Gray, A. M. and P. R. Flatt. 1998. Insulin-releashing and insulin-like activity of Agaricus campestris (mushroom). J. Endocrinol. 157, 259-266
  15. Ham, S. S., S. W. Oh, Y. K. Kim, K. S. Shin, K. Y. Chang and G. H. Chung. 2003. Antioxidant and genotoxic inhibition activity of ethanol extract from the Inonotus obliquus. J. Korean. Soc. Food. Sci. Nutr. 32, 1071-1075
  16. Jia, D. M., A. Tabaru, T. Akiyama, S. Ade and M. Otsuki. 2000. Fatty liver, chronic viral hepatities and autoimmune hepatities. Troglitazone prevents fatty changes of the liver in obese diabetic rats. J. Gastroentrol. Hepatol. 15, 1183-1191
  17. Kim, M. Y., M. H. Park and G. H. Kim. 1997. Effects of mushroom protein-bound polysaccharides on the blood glucose levels and energy metabolism in streptozotocin-induced diabetic rats. J. Kor. Nutr. 30, 743-750
  18. Kim, B. K., G. G. Shin, B. S. Jeong and J. Y. Chao 2001. Cholesterol-lowering effect of mushrooms powder in hyperlipidemic rats. J. Korean. Soc. Food. Sci. Nutr. 30, 510-515
  19. Konno, S., D. G. Tortorelis, S. A. Fullerton, A. A. Samadi, J. Hettiarachchi and H. Tazaki. 2001. A possible hypoglycaemic effect of Maitake mushroom on type 2 diabetic patients. Diabetic. Med. 18, 1010-1011
  20. Kubok, K., H. Aoki and N. Naba, 1994. Anti-diabetic activities present in the fruiting body of Gnfola frondosa (mitake). Biol. Pharm. Bull. 17, 1106-1110
  21. Lee, E. J. and H. D. Jang. 2004. Antioxidant activity and protective effect of five edible mushrooms on oxidative DNA damage. Food. Sci. Bioteehnol. 13, 443-449
  22. Lee, H. W., T. S. Park, J. W. Choi, S. Y. Yi and W. S. Shin. 2003. Antidiabetic effects of chitosan oligosaccharides in neonatal streptozotocin-induced noninsulin-dependent diabetes mellitus in rats. Biol. Pharm. Bull. 26, 1100-1103
  23. Lee, J. J., H. Y. Yi, J. W. Yang, J. S. Shin, J. H. Kwon and C. W. Kim. 2003. Characterization of streptozotocin-induced diabetic rats and pharmacodynamic activity of insulin formation. Biosci. Bioteehnol. Bioehem. 67, 2396-2401
  24. Lee, E. W., P. He, H. Kawagishi and K. Sugiyama. 2000. Suppression of D-galactosamine-induced liver injury by mushroom in rats. Biosei. Bioteehnol. Bioehem. 64, 2001-2002
  25. Lee, S. H., Y. M. Han, B. H. Min and J. S. Park. 2003. Cytoprotective effects of polyenoylphosphatidylcholine (PPC) on beta-cells during diabetic induction by streptozotocin. J. Histoehem. Cytoehem. 51, 1005-1015
  26. Lu, X. J., X. M. Chen, D. X. Fu, W. Cong and F. Ouyang. 2002. Effect of Amorphophallus konjae oligosaccharides on STZ-induced diabetic model of isolated islets. Life. Sci. 72, 711-719
  27. Mau, J. L., H. C. Un and S. F. Song. 2002. Antioxidant properties of several specially mushrooms. Food. Res. Inter. 35, 519-526
  28. Mizuno, T., C. Zhuang, K. Abe, H. Okamoto, T. Kiho, S. Ukai, S. Leclerc and J. Meijer. 1999. Antitumor and hypoglycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fe.) Pll. (Aphyllophoromycetideae). Int. J. Med. Mushrooms. 1, 301-316
  29. Mizuno, M., K. Morimoto and H. Tsuchida. 1993. Polysaccharides from Agurieus blazei stimulate lymphocyte T-cell subsets in mice. Biosei. Bioteehnol. Bioehem. 62, 434-437
  30. Miyake, Y., K. Yamamoto, N. Tsujihara and T. Osawa. 1998. Protective effects of lemon flavonoids on oxidative stree in diabetic rats. Lipids. 33, 689-695
  31. Nicotera, P. and S. Orrenius. 1986. Role of thiols in protection against biological reactive intermediates. Adv. Exp. Med. Biol. 197, 41-49
  32. Ohaeri, O. C. 2001. Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Bioscience. Reports. 21, 19-24
  33. Prince, P. S. M. and V. P. Menon. 2001. Antioxidant action of Tinospora eordifolia root extract in alloxan diabetic rats. Phytother. Res. 15, 213-218
  34. Sanders, R. A, F. M. Rauscher and J. B. Watkins. 2001. 3rd. Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J. Bioehem. Mol. Toxieol. 15, 143-149
  35. Talpur, N. A, B. W. Echard, A Y. Fan, O. Jaffari, D. Bagchi and H. G. Preuss. 2002. Antihypertensive and metabolic effects of whole Maitake mushroom powder and its fractions in two rat strains. Mol. Cell Bioehem. 237, 129-136
  36. Traverso, N., S. Menini, P. Odetti, M. A Pranzato, D. Cattalosso and U. M. Marinari. 1999. Lipidoperoxidation in hepatic subcellilar compartments of diabetic rats. Free. Radic. Bio. Med. 26, 538-547
  37. Uchiyama, M. and M. Mihara. 1978. Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86, 271-278
  38. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbial. Biotechnol. 60, 258-274
  39. Yang, B. K., D. H. Kim, S. C. Jeong, S. Das, Y. S. Choi, J. S. Shin, S. C. Lee and C. H. Song. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotechnol. Biochem. 66, 937-942
  40. Yuan, Z., P. He, J. Cui and H. Takeuchi. 1998. Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricula-judae Quel. on genetically diabetic KK-Ay mice. Biosci. Biotechnol. Biochem. 62, 1898-1903
  41. Zhang, G. L., Y. H. Wang, W. Ni, H. L. Teng and Z. B. Un. 2002. Hepatoprotective role of Ganodemza lucidum polysaccharide against BCG-induced immune liver injury in mice. World. J. Gastroenterol. 8, 728-733
  42. Zhang, H. N., J. H. He, L. Yuan and Z. B. Lin. 2003. In vitro and in vivo protective effects of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life. Sciences. 73, 2307-2319
  43. Zhang, H. N. and Z. B. Un. 2004. Hypoglycemic effect of Ganoderma lucidum polysaccharides. Acta. Pharmacal. Sin. 25, 191-195
  44. Cha, J. Y., Y. Mameda, K. Oogami, K. Yamamoto and T. Yanagita. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats. Biosci. Biotech. Biochem. 62, 508-513
  45. Jeon, B. S., J. W. Park, G. G. Shin, B. K. Kim, H. K. Kim, Y. S. Cho, J. Y. Chao 2004. Effect of fermented mushroom milk on hyperlipidemia and hepatic injury in streptozotocin-induced diabetic and Zucker diabetic fatty rats. Food. Sci. Biotechnol. 13, 576-580
  46. Maritim, A C., R. A Sanders and J. BWatkins. 2003. 3rd. Diabetes, oxidative stress, and antioxidants: a review. J. Bioehem. Mol. Toxieol. 17, 24-38
  47. Yamamoto, H., Y. Uchigata and H. Okamoto. 1981. DNA strand breaks in pancreatic islets by in vivo administration of alloxan or streptozotoxin. Biochem. Biopys. Res. Commun. 103, 1014-1020
  48. Baron, H. and P. S. Roheim. 1976. Hyperlipidemia in streptozotocin-treated rats. Diabetes. 25, 509-515
  49. Jeon, B.S., J. W. Park, B. K. Kim, H. K. Kim, T. S. Jung, J. R. Hahm, D. R. Kim, Y. S. Cho and J. Y. Chao 2005. Fermented mushroom milk supplemented dietary fiber prevents the onset of obesity and hypertriglyceridemia in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Diabetes. Obesity. Met. 7, 709-715

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