Cyanobacterial Diversity Analysis Using cpcBA-Intergenic Spacer Region

cpcBA-Intergenic Spacer Region을 이용한 Cyanobacteria의 다양성 분석

  • Choi Gang-Guk (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park Yong-Ha (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn Chi-Yong (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae Myoung-Sook (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh Hee-Mock (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 최강국 (한국생명공학연구원 생물자원센터) ;
  • 박용하 (한국생명공학연구원 생물자원센터) ;
  • 안치용 (한국생명공학연구원 환경생명공학연구실) ;
  • 배명숙 (한국생명공학연구원 환경생명공학연구실) ;
  • 오희목 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2005.12.01

Abstract

The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified rpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005, The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBA-IGS can be used for the species identification and community analysis of cyanobacteria.

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