The Biocidal Activity of Nano-sized Silver Particles Comparing with Silver Ion

은 이온과의 비교를 통한 나노 은 입자의 항균 특성 연구

  • Kim, Jee-Yeon (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Sung-Eun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Jae-Eun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Jong-Chan (School of Chemical and Biological Engineering, Seoul National University) ;
  • Yoon, Je-Yong (School of Chemical and Biological Engineering, Seoul National University)
  • 김지연 (서울대학교 화학생물공학부) ;
  • 김성은 (서울대학교 화학생물공학부) ;
  • 김재은 (서울대학교 화학생물공학부) ;
  • 이종찬 (서울대학교 화학생물공학부) ;
  • 윤제용 (서울대학교 화학생물공학부)
  • Published : 2005.07.31


In recent days, there is much interest in the biocidal activity of silver since silver is known to be safe and effective as disinfectant and biocidal material against coliforms and viruses. In particular, nano silted silver particles which can be used as effective biocidal material received more attention. Accordingly, it is important to investigate antimicrobial activity and mechanism of nano sized silver particles prepared in a cost-effective manner. In this study, nano sized silver particles were prepared via photoreduction of a silver salt ($AgNO_3$) in the bulk phase of $PEO_{20}-PPO_{70}-PEO_{20}$ (Pluronic 123) block copolymer The antimicrobial efficacy of silver nano particles against E. coli was investigated and compared with that of silver ion as the concentration of silver nano particles, pH ($5.6{\sim}8.2$), temperature ($4^{\circ}C{\sim}35^{\circ}C$) varied in aqueous system. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) was used to examine the nature of damaged microorganism with nano sized silver particles and silver ion. This study showed that antimicrobial efficacy of silver nano particles was approximately one twentieth than that of silver ion. It was more biocidal at higher pH in contrast with silver ion. In addition, nano silver particles was demonstrated to disrupt the outer membrane of E. coli, subsequently causing their aggregation. On the other hand, silver ion diffused into the cell damaging the cytoplasmic membrane without disrupting the outer membrane of E. coli.


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