Mechanism of Anti-inflammatory Effect of Peucedanum japonicum Thunb

방풍의 항염 효과 기전

  • Noh, Sung-Il (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Kim, Sang-Don (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Park, Sung-Cheul (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Seo, Byung-Yun (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Won-Kwang University) ;
  • Yeom, Seung-Ryong (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Young-Dal (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Shin, Byung-Cheul (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Song, Yung-Sun (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University)
  • 노성일 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김상돈 (원광대학교 한의과대학 한방재활의학교실) ;
  • 박성철 (원광대학교 한의과대학 한방재활의학교실) ;
  • 서병윤 (원광대학교 한의학전문 대학원 제3의학과) ;
  • 염승룡 (원광대학교 한의과대학 한방재활의학교실) ;
  • 권영달 (원광대학교 한의과대학 한방재활의학교실) ;
  • 신병철 (원광대학교 한의과대학 한방재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학교실)
  • Received : 2005.03.24
  • Accepted : 2005.04.20
  • Published : 2005.04.30
  • 264 0


Abstract

목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린을 첨가하여 배양하고 방품추출액을 투여하였다. 그 다음 MTT, CLISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-${\alpha}$, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-${\kappa}B$ 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비민세포에서 세포내 칼슘 수준과 NF-${\kappa}B$, TNF-${\alpha}$와 IL-6의 발현을 억제시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-${\alpha}$와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-${\kappa}B$의 활성을 억제하고 염증성 세포 활성 물질인 TNF-${\alpha}$와 IL-6의 분비도 억제하여 항염효과를 나타냄을 암시하고 있다.

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