Expression and diagnostic application of p12 protein of African swine fever virus by recombinant baculovirus

재조합 baculovirus에 의한 아프리카 돼지콜레라바이러스 p12 단백질의 발현과 진단적 적용

  • Choi, Kang-Seuk (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service) ;
  • Choi, Cheong-up (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service) ;
  • Kim, Yong-Joo (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service)
  • Accepted : 2005.02.21
  • Published : 2005.03.25

Abstract

African swine fever (ASF) is an infectious disease of domestic and wild pigs for which there is no vaccine in the world. A proper surveillance of viral activity and a timely response to ASF outbreaks depend upon the rapid diagnosis of ASF viral infection. Internationally prescribed enzyme-linked immunosorbent assay (ELISA) is a fast, sensitive test routinely used in the diagnosis of the ASF. However, inactivated whole ASF virus antigen used in this test is a tedious to prepare and has a risk of outside exposure of infectious virus by laboratory accident during the preparation. An ASF virus noninfectious recombinant antigen is a safe and easily produced alternative antigen for use in diagnostic assay. We have cloned the ORF O61R gene of the ASF virus to generate a recombinant baculovirus producing the p12 protein in insect cells under control of the polyhedrin promoter as non-fusion protein. When used in an indirect ELISA, the p12 antigen showed reactivity with all known ASF positive pig sera but not with negative pig sera. Our results indicated that the p12 protein would be one of alternative antigens for diagnosis of the ASF.

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